FDPS Antibody

Code CSB-PA008563LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
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  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate
    All lanes: FDPS antibody at 3µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 49, 41 kDa
    Observed band size: 41 kDa

  • Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA008563LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA008563LA01HU at dilution of 1:100

  • Immunoprecipitating FDPS in HepG2 whole cell lysate
    Lane 1: Rabbit control IgG (1µg) instead of CSB-PA008563LA01HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-PA008563LA01HU (8µg) + HepG2 whole cell lysate (500µg)
    Lane 3: HepG2 whole cell lysate (10µg)

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) FDPS Polyclonal antibody
Uniprot No. P14324
Target Names FDPS
Alternative Names (2E antibody; (2E,6E) farnesyl diphosphate synthase antibody; 6E)-farnesyl diphosphate synthase antibody; Dimethylallyltranstransferase antibody; Farnesyl diphosphate synthase antibody; Farnesyl diphosphate synthetase antibody; Farnesyl pyrophosphate synthase antibody; Farnesyl pyrophosphate synthetase antibody; Fdps antibody; FPP synthase antibody; FPP synthetase antibody; FPPS antibody; FPPS_HUMAN antibody; FPS antibody; Geranyltranstransferase antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Farnesyl pyrophosphate synthase protein (1-419AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IP 1:200-1:2000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Structure-based virtual screening and biological evaluation of novel non-bisphosphonate farnesyl pyrophosphate synthase inhibitors. Qingzhu Liu, et al,European Journal of Medicinal Chemistry,2019

Sample type: Cancer cell lines
Sample species: Human
Antibody dilution factor: 1:2000
Application: WB
Review: Western blot analysis of FPPS expression in various types of cancer cell lines, including colorectal cancers (HCT116, LoVo), breast cancers (MDA-MB-231, MDA-MB-468, MCF-7), prostate cancer (PC-3), liver cancer (HepG2), and ovarian cancer (SKOV-3).

Target Data

Function Key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. FPP also serves as substrate for protein farnesylation and geranylgeranylation. Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate.
Gene References into Functions
  1. Farnesyl pyrophosphate (FPP) allosterically regulated the activity of farnesyl pyrophosphate synthase. PMID: 28098152
  2. FPPS mediates TGF-beta1-induced lung cancer cell invasion and epithelial-to-mesenchymal transition via the RhoA/Rock1 pathway. PMID: 29337059
  3. Crystallographic and thermodynamic characterization of phenylaminopyridine bisphosphonates binding to human farnesyl pyrophosphate synthase PMID: 29036218
  4. These results are consistent with the previously proposed hypothesis that the allosteric pocket of human FPPS, located near the active site, plays a feed-back regulatory role for this enzyme. PMID: 25630225
  5. our study indicated that DR patients have higher VEGF levels than diabetic patients without retinopathy, and -2578A/C (rs699947) and +405C/G (rs2010963) may be important factors in determining serum VEGF levels. PMID: 24534219
  6. Results suggest that polymorphisms of the FDPS gene may influence the bone response to drugs targeting the mevalonate pathway, like statins. PMID: 24311107
  7. A co-crystal structure of human farnesyl pyrophosphate synthase in complex with a bisphosphonate and two molecules of inorganic phosphate. PMID: 24598914
  8. The results identify new classes of FPPS inhibitors, diterpenoids and sesquiterpenoids, that bind to the IPP site and may be of interest as anticancer and antiinfective drug leads. PMID: 24927548
  9. These observations suggest that an increase in the expression of endogenous FPPS could confer at least partial resistance to the pharmacological effect of N-BP drugs such as ZOL in vivo PMID: 24369118
  10. LRP5 and FDPS loci age-specifically affect skeletal traits in healthy fertile women. PMID: 23238007
  11. Data indicate compounds represent a new structural class of farnesyl pyrophosphate synthase (hFPPS) inhibitors and suggest a development of therapeutics. PMID: 23998921
  12. The iPA-driven modulation of FDPS can cause an enhancement of post-translational prenylation essential for the biological activity of key proteins in NK signaling and effector functions, such as Ras. PMID: 23847096
  13. FPPS was more highly expressed in prostate cancer vs. normal prostate tissue. The association of FPPS with established histopathological risk parameters and biochemical recurrence implicates a contribution of the mevalonate pathway to PC progression. PMID: 22407328
  14. FPPS might play an important role in Ang II-induced cardiac hypertrophy and fibrosis in vivo, at least in part through RhoA, p-38 MAPK and TGF-beta1. PMID: 23277274
  15. The crystal structure of human FPPS in complex with a novel bisphosphonate YS0470 and in the absence of a second substrate showed partial ordering of the tail in the closed conformation. PMID: 23234314
  16. Common polymorphisms of the FDPS gene influence the response to bisphosphonates in osteoporotic women. PMID: 21151198
  17. findings reveal a FDPS-dependent mechanism in the internalization and down-regulation of beta2AR, identify FDPS as a potential target for improving the therapeutic efficacy of beta-agonists PMID: 22278941
  18. first study on the gene FDPS rs2297480 SNP in postmenopausal Thai women.The effect did not contribute to the baseline of bone mineral density nor bone turnover markers. PMID: 22338925
  19. The A/C rs2297480 polymorphism of FDPS was highly differently distributed among osteonecrosis-of-the-jaw patients and controls, with a correlation between AA carrier status and occurrence of ONJ after 18-24 months of treatment with bisphosphonates. PMID: 21196316
  20. characterized the sterol-response-element-binding protein 2 and nuclear factor Y-binding site in the farnesyl diphosphate synthase promoter PMID: 20450493
  21. This study provides the first evidence of the presence of FPPs activity in human CRC. Moreover, FPPs enzyme was found to play a significant role in colon cancer proliferation. PMID: 15713990
  22. mitochondrial targeting of FPS may be widespread among eukaryotes PMID: 17198737
  23. findings suggest that a single nucleotide polymorphism in the FDPS gene (rs2297480) may be a genetic marker for lower bone mineral density in postmenopausal Caucasian women PMID: 17368768
  24. FDPS is involved in the resistance to zoledronic acid of osteosarcoma cells. PMID: 18494934
  25. characterized functionally the minimal basal promoter of the human FDPS gene by means of deletion mutants and we have identified two cis-acting elements which modulate the FDPS gene expression and are recognized by Pax5 and OCT-1 transcription factors PMID: 19056481
  26. FPPS knockdown cells activated Vgamma9Vdelta2 T cells, as measured by increased levels of CD69 and CD107a, killing of FPPS knockdown cells, and induction of IFN-gamma secretion PMID: 19494338

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Involvement in disease Porokeratosis 9, multiple types (POROK9)
Subcellular Location Cytoplasm
Protein Families FPP/GGPP synthase family
Database Links

HGNC: 3631

OMIM: 134629

KEGG: hsa:2224

STRING: 9606.ENSP00000349078

UniGene: Hs.335918

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