FMN1 Antibody

Datasheet

Code	CSB-PA715012LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: K562 whole cell lysate, A549 whole cell lysate All lanes: FMN1 antibody at 3.9µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 158, 72, 54, 133 kDa Observed band size: 158 kDa IHC image of CSB-PA715012LA01HU diluted at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CSB-PA715012LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) FMN1 Polyclonal antibody

Uniprot No.

Q68DA7

Target Names

FMN1

Alternative Names

FMN1 antibody; FMN antibody; LDFormin-1 antibody; Limb deformity protein homolog antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Formin-1 protein (354-487AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The FMN1 Antibody (Product code: CSB-PA715012LA01HU) is Non-conjugated. For FMN1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA715012LB01HU	FMN1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA715012LC01HU	FMN1 Antibody, FITC conjugated
Biotin	CSB-PA715012LD01HU	FMN1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Plays a role in the formation of adherens junction and the polymerization of linear actin cables.

Gene References into Functions

Formin-1 (FMN1) was shown to reside in the nucleus and studies indicate that some formins can shuttle in and out of the nucleus. PMID: 27913680
actin arcs populating the medial, lamella-like region of the immunological synapse (IS) arise from linear actin filaments generated by one or more formins present at the IS distal edge. PMID: 27799367
Filopodia initiation: focus on the Arp2/3 complex and formins. PMID: 21975549
Chromosomal imbalances in the GREM1 FMN1 region in individuals with limb defects are reported here. PMID: 20610440
The obtained data indicate the possibility of participation of polymorphism in gene FMN1 in formation of predisposition to prostate cancer. PMID: 20540360
Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
Observational study of gene-disease association. (HuGE Navigator) PMID: 20540360
Observational study of gene-disease association. (HuGE Navigator) PMID: 20200978
both mammalian Spir proteins, Spir-1 and Spir-2, interact with mammalian Fmn subgroup proteins formin-1 and formin-2 PMID: 19605360
Observational study of gene-disease association. (HuGE Navigator) PMID: 19096130

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Subcellular Location

Nucleus. Cytoplasm. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Protein Families

Formin homology family, Cappuccino subfamily

Database Links

HGNC: 3768

OMIM: 136535

KEGG: hsa:342184

STRING: 9606.ENSP00000333950

UniGene: Hs.657649

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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