GOT1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA284562A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: HEK293 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), LO2 whole cell lysate(30µg), Hela whole cell lysate(30µg), A549 whole cell lysate(30µg), Raji whole cell lysate(30µg), U-87MG whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg) All lanes: GOT1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time：2min Immunofluorescence staining of HepG2 cell with CSB-RA284562A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA284562A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

P17174

Target Names

GOT1

Alternative Names

Aspartate aminotransferase, cytoplasmic (cAspAT) (EC 2.6.1.1) (EC 2.6.1.3) (Cysteine aminotransferase, cytoplasmic) (Cysteine transaminase, cytoplasmic) (cCAT) (Glutamate oxaloacetate transaminase 1) (Transaminase A), GOT1

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human GOT1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4E2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

GOT1, also known as cytoplasmic aspartate aminotransferase, plays a central role in amino acid metabolism and the malate-aspartate shuttle, facilitating the transfer of reducing equivalents across the mitochondrial membrane. This enzyme has garnered significant research attention due to its involvement in cancer metabolism, where tumor cells often rewire transamination pathways to support rapid proliferation. Understanding GOT1 expression and localization provides valuable insights into metabolic reprogramming in both normal physiology and disease states.

This recombinant monoclonal antibody, clone 4E2, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity, ensuring that optimization efforts translate reliably across experiments and over time.

Validation data demonstrates robust performance across multiple applications. Western blot analysis detects a clean band at the predicted 46 kDa molecular weight across an extensive panel of human cell lines including HEK293, HepG2, LO2, HeLa, A549, Raji, and U-87MG, as well as mouse and rat brain tissue lysates, confirming cross-species reactivity with human, mouse, and rat samples. Immunofluorescence staining in HepG2 cells reveals cytoplasmic localization consistent with the enzyme's known subcellular distribution, while flow cytometry analysis in HeLa cells shows clear separation from isotype control, enabling quantitative assessment of GOT1 expression at the single-cell level.

Whether investigating metabolic dependencies in cancer research, exploring neurodegenerative conditions where amino acid metabolism is disrupted, or characterizing cellular stress responses, this antibody provides the flexibility to move seamlessly between detection platforms while maintaining confidence in your results.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.

Gene References into Functions

high transaminase elevations do not predict severe complications or reflect remnant ischemic area. PMID: 29553104
Missense variant (p.Gln208Glu, rs374966349) in glutamate oxaloacetate transaminase 1 (GOT1) was found, as a putative causal variant predisposing to Familial Macro-aspartate aminotransferase . PMID: 28716744
HIF-2a regulates non-canonical glutamine metabolism via activation of PI3K/mTORC2 pathway and GOT1 expression in human pancreatic ductal adenocarcinoma. PMID: 28544376
ALT is a more suitable index than AST for developing a regression model. PMID: 28628604
High AST1 expression is associated with high mortality in hemodialysis patients. PMID: 26333544
GOT1 plays an important role in energy metabolism and ROS balance in chronic acidosis stress in pancreatic tumor cells. PMID: 28455244
Early abnormal liver enzyme levels of aspartate aminotransferase and alanine aminotransferase may increase the prevalence of human cytomegalovirus antigenemia after hematopoietic stem cell transplantation. PMID: 28415934
Elevated preoperative aspartate aminotransferase-to-platelet ratio index may be independently associated with poor overall survival and disease-free survival in hepatocellular carcinoma patients following curative resection PMID: 28351330
Elevated levels of ALT are Associated with Cardiovascular disease. PMID: 27872510
Elevated aspartate aminotransferase level was associated with hepatocellular carcinoma. PMID: 26881519
The serum hepcidin, ferritin, alanine transaminase, aspartate transaminase, gamma-glutamyltransferase and bilirubin were examined in two independent Chinese cohorts consisted of 3455 individuals. PMID: 26290281
Data suggest that, in context of abnormal hepatic lipid accumulation in nonalcoholic fatty liver disease, circulating GOT1 rises due to up-regulation of hepatic expression of GOT1 associated with greater gluconeogenesis and insulin resistance. PMID: 26791191
Human GOT1 was expressed in E coli, purified by affinity chromatography and preliminary crystallographic studies were done. PMID: 26003525
We propose the GOT1 expression status as a simple and reliable prognostic biomarker in pancreatic ductal adenocarcinoma PMID: 25595905
Significantly higher GOT levels were found in the 1-20 year age group of type 1 PMID: 24849491
The aim of this study was to establish the reference intervals (RIs) of total bilirubin (TBIL), alanine aminotransferase (ALT), aspartate transaminase (AST), and creatinine (CREA) for apparently healthy elderly (Han ethnicity) in Shuyang, China. PMID: 25272068
APRI and AST/ALT are independent predictors for significant fibrosis in chronic hepatitis C patients. PMID: 22352690
This study highlights the necessity of intensive follow-up for those with elevated AST and ALT levels and comorbid alcohol use disorder for preventing excessive natural deaths. PMID: 22242166
we discovered a rare in-frame deletion in GOT1 gene, which inactivates cAST enzyme in the Old Order Amish. PMID: 21900944
Data indicate that the percentages of CD4(+)CXCR5(+) TFH in IA patients were positively correlated with AST. PMID: 21750724
liver enzymes, including AST, were significantly associated with IR according to direct clamp assessment, which were independent of the traditional risk factors in diabetic patients PMID: 20972737
Elevated serum LDH isoenzymes and AST indicate a disturbance (of uncertain clinical significance) within multiple extraosseous tissues when there is CLCN7 deficiency. PMID: 20499337
mildly elevated in severe acute respiratory syndrome patients during second week after onset of fever. PMID: 15306699
saliva AST and ALP may have roles in development of periodontal diseases PMID: 16681433
PTLP activity is positively associated with serum ALT1, GOT1 and GOT2 in type 2 diabetes mellitus and metabolic syndrome. PMID: 17877759
Abnormal serum aminotransferase values are uncommon in severely obese children in France. PMID: 17907318
Data revealed higher aspartate aminotransferase for women with a family history of diabetes, when adjusted for age and BMI. PMID: 18242760
Serum bilirubin, alkaline phosphatase, and aspartate aminotransferase are an efficient set of biochemistries to identify UDCA-treated patients with primary biliary cirrhosis at risk of death or liver transplantation (LT). PMID: 18752324
Neither ALT nor GGT concentrations were correlated with ALP concentration, but AST concentration was moderately correlated with ALP concentration. PMID: 19012630
Results suggest that alanine and aspartate aminotransferase levels and AST/ALT ratio do not seem to be reliable predictors for non-alcoholic steatohepatitis. PMID: 19370784
the management of patients with pyelonephritis should take into account that moderate and self-limited abnormalities in aminotransferase levels are frequent during the acute phase of the disease PMID: 19393479

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Subcellular Location

Cytoplasm.

Protein Families

Class-I pyridoxal-phosphate-dependent aminotransferase family

Database Links

HGNC: 4432

OMIM: 138180

KEGG: hsa:2805

STRING: 9606.ENSP00000359539

UniGene: Hs.500756

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No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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