INO80 Antibody

Code CSB-PA892466LA01HU
Size US$166
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  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate
    All lanes: INO80 antibody at 2.9μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 177 kDa
    Observed band size: 177 kDa

  • Immunofluorescence staining of Hela cells with CSB-PA892466LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) INO80 Polyclonal antibody
Uniprot No.
Target Names
INO80
Alternative Names
DNA helicase INO80 antibody; hINO80 antibody; homolog of yeast INO80 antibody; INO80 antibody; INO80 complex subunit A antibody; INO80 homolog antibody; INO80_HUMAN antibody; INO80A antibody; INOC1 antibody; KIAA1259 antibody; Putative DNA helicase INO80 complex homolog 1 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Chromatin-remodeling ATPase INO80 protein (1365-1495AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The INO80 Antibody (Product code: CSB-PA892466LA01HU) is Non-conjugated. For INO80 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA892466LB01HU INO80 Antibody, HRP conjugated ELISA
FITC CSB-PA892466LC01HU INO80 Antibody, FITC conjugated
Biotin CSB-PA892466LD01HU INO80 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
ATPase component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and DNA repair. Binds DNA. As part of the INO80 complex, remodels chromatin by shifting nucleosomes. Regulates transcription upon recruitment by YY1 to YY1-activated genes, where it acts as an essential coactivator. Involved in UV-damage excision DNA repair. The contribution to DNA double-strand break repair appears to be largely indirect through transcriptional regulation. Involved in DNA replication. Required for microtubule assembly during mitosis thereby regulating chromosome segregation cycle.
Gene References into Functions
  1. human INO80 chromatin remodeling complex is more compositionally heterogenous at its genomic targets than anticipated. PMID: 29432129
  2. structure of the human INO80 chromatin remodeller with a bound nucleosome, which reveals that INO80 interacts with nucleosomes in a previously undescribed manner PMID: 29643506
  3. INO80-silencing MSC cultured in osteogenic condition expressed lower mRNA levels of osteoblast-specific genes, including Runx2, Osx, Col1alpha1 and OCN. INO80 can interact with Wdr5 in MSC and positively regulates the canonical Wnt signaling transduction. PMID: 27804957
  4. Instead nucleosome sliding requires cooperativity between two INO80 complexes that monitor DNA length simultaneously on either side of the nucleosome during sliding. The C-terminal domain of the human Ino80 subunit (Ino80CTD) binds cooperatively to DNA and dimerisation of these domains provides crosstalk between complexes. PMID: 28585918
  5. Ino80 was upregulated in cervical cancer and promoted cell proliferation and tumorigenesis by binding to the Nanog transcription start site and enhancing its expression. PMID: 27750218
  6. By demonstrating a key role for SUMOylation in regulating the INO80 chromatin remodeling complex, this work illustrates the power of bioinformatics analysis of large data sets in predicting novel biological phenomena. PMID: 28254775
  7. Our work defines SETD2 as a tumor suppressor gene in Hepatosplenic T-cell lymphoma (HSTL)and implicates genes including INO80 and PIK3CD in the disease PMID: 28122867
  8. our study defines a critical role of INO80 in promoting oncogenic transcription and NSCLC tumorigenesis, and reveals a potential treatment strategy for inhibiting the cancer transcription network by targeting the INO80 chromatin remodeling complex. PMID: 27641337
  9. Interactions of the chromatin remodeling protein INO80 with DNA, and the role of YY1 in this process have been described. PMID: 27428271
  10. The inositol hexaphosphate binding site is located within the C-terminal region of the Ino80 subunit. PMID: 27257055
  11. the existence of both INO80 and YY1 is required for recruiting the INO80/YY1 complex to BCCIP promoter region. Our findings strongly indicate that BCCIP is a potential target gene of the INO80/YY1 complex. PMID: 27535137
  12. The expression of Ino80, the SWI/SNF ATPase, is elevated in melanoma cells and patient melanomas compared with normal melanocytes and benign nevi. PMID: 27340176
  13. Overexpression of miRNA-148a and knockdown of INO80 acted synergistically to decrease the expression of stem cell marker genes as well as to attenuate stem cell-specific properties including the ability to form tumors. PMID: 27779717
  14. INO80 complex negatively regulates p21 expression in a p53-mediated mechanism and is implicated in cell cycle phase G2/M arrest and abnormal chromosome stability. PMID: 26340092
  15. INO80 is stabilized and targeted to replication forks by BAP1 during normal DNA synthesis but downregulated in BAP1 defective cancer cells PMID: 25283999
  16. Mutations in INO80D is associated with translocation renal cell carcinoma. PMID: 24899691
  17. The data indicate that under conditions of replication stress INO80 protects stalled forks from collapsing and allows their subsequent restart. PMID: 25016522
  18. human Inositol auxotrophy 80 (Ino80) SNF2 ATPase is subject to regulation at multiple levels in the INO80 chromatin-remodeling complex. PMID: 24297934
  19. These results suggest that the human INO80 complex, like the yeast complex, was involved in the DNA damage tolerance pathway and that phosphorylation of human INO80 was involved in the DNA damage tolerance pathway. PMID: 22166198
  20. These results suggest that hIno80 can play a direct role in the spindle assembly independent of its chromatin remodeling activity. PMID: 22133677
  21. Ino80 mediates double-strand break repair through its role in DNA end strand resection PMID: 21947284
  22. Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling. PMID: 21303910
  23. These results suggest that hINO80 assists double-strand break repair by positively regulating the expression of the Rad54B and XRCC3 genes. PMID: 20687897
  24. hINO80 associates with spindle microtubule during mitosis, and its deficiency leads to defective microtubule assembly and abnormal chromosome segregation. PMID: 20237820
  25. report the cloning, expression, and functional activity of the domains from hINO80 gene both in terms of the DNA dependent ATPase as well as DNA binding activity PMID: 16298340
  26. Methods for purification and assay of the INO80 chromatin remodeling complexes are described. PMID: 17101442
  27. Binding of YY1 to its DNA sites in target genes requires INO80, suggesting that YY1 uses the INO80 complex not only to activate transcription but also to gain access to target promoters PMID: 17721549
  28. Ino80 has a crucial role in stabilizing a stalled replisome to ensure proper restart of DNA replication PMID: 18376411
  29. INO80 is an important new player in DNA replication to help safeguard the integrity of the genome PMID: 18388905
  30. In the nucleus Uch37 is also associated with the human Ino80 chromatin-remodeling complex (hINO80). PMID: 18922472
  31. Identifies INOC1 gene product as a SNF2-like helicase associated with human INO80 chromatin remodeling complex (hINO80). hINO80 includes orthologs of 8 subunits of the yeast INO80 complex, as well as additional, metazoan-specific proteins. PMID: 16230350

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Subcellular Location
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Chromosome.
Protein Families
SNF2/RAD54 helicase family
Tissue Specificity
According to PubMed:10574462, widely expressed. According to PubMed:16298340, specifically expressed in brain, liver and pancreas.
Database Links

HGNC: 26956

OMIM: 610169

KEGG: hsa:54617

STRING: 9606.ENSP00000355205

UniGene: Hs.292949

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