INO80 Antibody

Datasheet
Code CSB-PA892466LA01HU
See More Details
Size US$299
Uniprot No. Q9ULG1
Image
  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate
    All lanes: INO80 antibody at 2.9μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 177 kDa
    Observed band size: 177 kDa

  • Immunofluorescence staining of Hela cells with CSB-PA892466LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunogen Recombinant Human Chromatin-remodeling ATPase INO80 protein (1365-1495AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200
Relevance ATPase component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and DNA repair (PubMed:16230350, PubMed:16298340, PubMed:17721549, PubMed:20855601, PubMed:20237820). Binds DNA (PubMed:16298340, PubMed:21303910). As part of the INO80 complex, remodels chromatin by shifting nucleosomes (PubMed:16230350, PubMed:21303910). Regulates transcription upon recruitment by YY1 to YY1-activated genes, where it acts as an essential coactivator (PubMed:17721549). Involved in UV-damage excision DNA repair (PubMed:20855601). The contribution to DNA double-strand break repair appears to be largely indirect through transcriptional regulation (PubMed:20687897). Involved in DNA replication (PubMed:20237820). Required for microtubule assembly during mitosis thereby regulating chromosome segregation cycle (PubMed:20237820).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Chromatin-remodeling ATPase INO80 (hINO80) (DNA helicase-related INO80 complex homolog 1) (DNA helicase-related protein INO80) (INO80 complex subunit A), INO80, INO80A, INOC1, KIAA1259
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Research Area Epigenetics and Nuclear Signaling
Target Names INO80
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function DNA helicase and probable main scaffold component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; according to PubMed
Subcellular Location Nucleus
Protein Families SNF2/RAD54 helicase family
Tissue Specificity According to PubMed:10574462, widely expressed. According to PubMed:16298340, specifically expressed in brain, liver and pancreas.
Database Links

HGNC: 26956

OMIM: 610169

KEGG: hsa:54617

STRING: 9606.ENSP00000355205

UniGene: Hs.292949

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