Product Details

Uniprot No.

Target Names

MAGED1

Alternative Names

DLXIN 1 antibody; MAGD1_HUMAN antibody; MAGE D1 antigen antibody; MAGE tumor antigen CCF antibody; MAGE-D1 antigen antibody; Maged1 antibody; Melanoma antigen family D 1 antibody; Melanoma associated antigen D1 antibody; Melanoma-associated antigen D1 antibody; Neurotrophin receptor interacting MAGE homolog antibody; Neurotrophin receptor-interacting MAGE homolog antibody; NRAGE antibody; PP2250 antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Human MAGED1

Immunogen Species

Homo sapiens (Human)

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.

Tested Applications

ELISA,WB,IHC

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Inhibits cell cycle progression, and facilitates NGFR-mediated apoptosis. May act as a regulator of the function of DLX family members. May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Plays a role in the circadian rhythm regulation. May act as RORA co-regulator, modulating the expression of core clock genes such as ARNTL/BMAL1 and NFIL3, induced, or NR1D1, repressed.

Gene References into Functions

Our results indicate that NRAGE subcellular localization is related to radiation resistance of esophageal carcinoma cell and EMT may be involved in NRAGE subcellular location. PMID: 29516958
NRAGE upregulation was correlated with advanced TNM stage, local invasion, and poor survival. Importantly, NRAGE could serve as an independent prognostic factor in patients with gastric cancer. PMID: 29778424
The nuclear localized NRAGE interacts with RNF8 and BARD1 to mediate the resistance of esophageal carcinomas cells against DNA-damaging agents. PMID: 27035619
MAGED1 binds and positively regulates the transcriptional activity of family members SIM1, SIM2, NPAS4 and ARNT2, but does not interact with AhR, HIF1alpha and ARNT. This interaction is mediated by PAS repeat regions which also form the interface for bHLH PAS dimerisation, and accordingly MAGED1 is not found in complex with bHLH PAS dimers. PMID: 27472814
our surprise, NRAGE induces nuclear localization of beta-catenin and increases its DNA binding ability. Further studies reveal that NRAGE leads to the modification of beta-catenin/Arm with O-linked beta-N-acetylglucosamine (O-GlcNAc), and failure of the association between beta-catenin/Arm and pygopus(pygo) protein, which is required for transcriptional activation of Wnt target genes. Therefore, our findings suggest a ... PMID: 28427939
Loss of GSPT2 and/or MAGED1 function may contribute to the intellectual disability. PMID: 28414775
The ectopic subcellular localization of NRAGE mediated nuclear translocation of beta-catenin. PMID: 26738870
High NRAGE expression is associated with esophageal carcinomas. PMID: 24710624
MAGE-D1 plays important roles in the central nervous system in both developmental and adult stages. PMID: 23314527
Univariate and multivariate analyses indicated that MAGED1 expression was an independent prognostic factor in colorectal carcinoma PMID: 22935435
NRAGE may participate in the formation of radioresistance of TE13R120 cells by changing its subcellular localization, but its relationship with cell apoptosis has not been confirmed. PMID: 20868560
we establish the roles for Dlxin-1, one as an anti-tumorigenic and anti-invasive protein in high-grade gliomas and the other as an inducer of differentiation of glioma stem cells. PMID: 21109781
Data report that NRAGE, via the same XIAP-Tak1-Tab1 complex, is required for the phosphorylation of IKK -alpha/beta and subsequent transcriptional activation of the p65 subunit of NF-kappaB. PMID: 20100315
A RING finger protein Praja1 regulates Dlx5-dependent transcription through its ubiquitin ligase activity for the Dlx/Msx-interacting MAGE/Necdin family protein, Dlxin-1. PMID: 11959851
hNRAGE arrests cell growth through a p53 dependent pathway. hNRAGE also increases the p53 protein level as well as its phosphorylation (Ser392). PMID: 15094062
The expression pattern of Maged1 roughly summarizes that of Maged2 and Maged3 PMID: 15162511
MAGE-D1 protein expression was reduced in 6 of 16 breast carcinoma cell lines as compared with untransformed immortal mammary epithelial cell lines; suppression of MAGE-D1 expression may be involved in the tumorigenesis of some sporadic breast cancers. PMID: 15930293
demonstrate the importance of human NRAGE in homotypic cell-to-cell adhesion and illuminate the mechanism of human NRAGE in the process of inhibition of cell adhesion PMID: 16125672
Data show that hNRAGE gene can inhibit the growth of 293 cells. PMID: 16388736
studies indicate for the first time that NRAGE could suppress metastasis of melanoma and pancreatic cancer probably through downregulation of MMP-2 PMID: 17140727
MAGE-D1 might be a novel inhibitor of angiogenesis in vitro and in vivo. PMID: 17149546
These findings provide new insight into the ability of MAGE-D1 to suppress the motility and adhesion response of tumor cells by interfering with actin cytoskeleton reorganization and hypoxia inducible factor 1-dependent gene expression. PMID: 17453828
Che-1 interacts with NRAGE and NRAGE overexpression downregulates endogenous Che-1 by targeting it for proteasome-dependent degradation. PMID: 17488777
Enhanced expression of MAGE-D1 by gene transfer could reverse the malignant phenotypes of breast cancer cells. PMID: 19639218