MAP3K7 (Ab-187) Antibody

Datasheet
Code CSB-PA173975
Product Type Polyclonal Antibody
Size US$297
Uniprot No. O43318
Image
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MAP3K7 (Ab-187) antibody.
  • Western blot analysis of extracts from Jurkat cells, treated with heat shock, using MAP3K7 (Ab-187) antibody.
  • Western blot analysis of extracts from JK cells (Lane 2), using MAP3K7 (Ab-187) antiobdy. The lane on the left is treated with synthesized peptide.
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunogen Synthesized non-phosphopeptide derived from Human MAP3K7 around the phosphorylation site of threonine 187 (H-M-T(p)-N-N).
Raised in Rabbit
Species Reactivity Human,Mouse
Specificity The antibody detects endogenous levels of total MAP3K7 protein.
Tested Applications ELISA,WB,IHC;WB:1:500-1:3000,IHC:1:50-1:100
Relevance Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs, c-jun N-terminal kinases (JNKs) and I-kappa-B kinase complex (IKK). Both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1), while nuclear factor-kappa B is activated by IKK. MAP3K7 activates also IKBKB and MAPK8/JNK1 in response to TRAF6 signaling and mediates BMP2-induced apoptosis. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK1, but not that of NF-kappa-B. Promotes TRIM5 capsid-specific restriction activity.

Sakurai H., Biochem. Biophys. Res. Commun. 243:545-549(1998).
Mungall A.J., Nature 425:805-811(2003).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality Polyclonal
Alias EC 2.7.11.25; kinase TAK1; M3K7; MAP3K7; Mitogen-activated protein kinase kinase kinase 7
Immunogen Species Homo sapiens (Human)
Target Names MAP3K7
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs, c-jun N-terminal kinases (JNKs) and I-kappa-B kinase complex (IKK). Both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1), while nuclear factor-kappa B is activated by IKK. MAP3K7 activates also IKBKB and MAPK8/JNK1 in response to TRAF6 signaling and mediates BMP2-induced apoptosis. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK1, but not that of NF-kappa-B. Promotes TRIM5 capsid-specific restriction activity.
Involvement in disease Frontometaphyseal dysplasia 2 (FMD2); Cardiospondylocarpofacial syndrome (CSCF)
Subcellular Location Cytoplasm, Cell membrane, Peripheral membrane protein, Cytoplasmic side
Protein Families Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily
Tissue Specificity Isoform 1A is the most abundant in ovary, skeletal muscle, spleen and blood mononuclear cells. Isoform 1B is highly expressed in brain, kidney and small intestine. Isoform 1C is the major form in prostate. Isoform 1D is the less abundant form.
Database Links

HGNC: 6859

OMIM: 157800

KEGG: hsa:6885

STRING: 9606.ENSP00000358335

UniGene: Hs.594838

Pathway MAPK signaling pathway
Wnt signaling pathway
NF-kappa B signaling pathway
TNF signaling pathway
Adherens junction
Autophagy
IL-17 signaling pathway
NOD-like receptor signaling pathway
Osteoclast differentiation
T cell receptor signaling pathway
Toll-like receptor signaling pathway
AMPK signaling

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