MAP3K7 (Ab-187) Antibody

Code CSB-PA173975
Size US$297
Image
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MAP3K7 (Ab-187) antibody.
  • Western blot analysis of extracts from Jurkat cells, treated with heat shock, using MAP3K7 (Ab-187) antibody.
  • Western blot analysis of extracts from JK cells (Lane 2), using MAP3K7 (Ab-187) antiobdy. The lane on the left is treated with synthesized peptide.
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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) MAP3K7 Polyclonal antibody
Uniprot No. O43318
Target Names MAP3K7
Alternative Names M3K7_HUMAN antibody; MAP3K 7 antibody; Map3k7 antibody; MEKK7 antibody; Mitogen activated protein kinase kinase kinase 7 antibody; Mitogen-activated protein kinase kinase kinase 7 antibody; TAK1 antibody; TGF beta activated kinase 1 antibody; TGF-beta-activated kinase 1 antibody; TGF1a antibody; Transforming growth factor beta activated kinase 1 antibody; Transforming growth factor-beta-activated kinase 1 antibody
Raised in Rabbit
Species Reactivity Human,Mouse
Immunogen Synthesized non-phosphopeptide derived from Human MAP3K7 around the phosphorylation site of threonine 187 (H-M-T(p)-N-N).
Immunogen Species Homo sapiens (Human)
Clonality Polyclonal
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Tested Applications ELISA,WB,IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:3000
IHC 1:50-1:100
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs, c-jun N-terminal kinases (JNKs) and I-kappa-B kinase complex (IKK). Both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1), while nuclear factor-kappa B is activated by IKK. MAP3K7 activates also IKBKB and MAPK8/JNK1 in response to TRAF6 signaling and mediates BMP2-induced apoptosis. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK1, but not that of NF-kappa-B. Promotes TRIM5 capsid-specific restriction activity.
Gene References into Functions
  1. De novo splicing variant in MAP3K7 was identified in a patient with cardiospondylocarpofacial syndrome with features of hereditary connective tissue disorder. PMID: 29467388
  2. TAK1 plays a central role in promoting triple-negative breast cancer cell adaptation to the lung microenvironment by facilitating positive feedback signaling mediated by P38. PMID: 29777109
  3. In brief, TAK1 can function as direct AMPK upstream kinase in specific contexts and in response to a subset of TAK1 activating stimuli. Further research is needed to define the intricate signals that are conditional for TAK1 to phosphorylate and activate AMPKalpha at T172. [review] PMID: 30111748
  4. The expression of IL-6 gene and protein was significantly induced by IL-17F. IL-17F activated TAK1 and NF-kappaB in airway smooth muscle cells. PMID: 28474507
  5. overexpression of miR-20a reduced colony formation and tumor growth. Furthermore, the data revealed that the function of miR-20a was probably exerted via targeting the TAK1 expression. Overexpression of miR-20a sensitizes the osteosarcoma cells to chemotherapeutic drugs. PMID: 29327611
  6. TGFbeta and IL1beta signaling interact at the SMAD2/3 level in human primary MSC. Down-stream TGFbeta target genes were repressed by IL1beta independent of C-terminal SMAD2 phosphorylation. We demonstrate that SMAD2/3 linker modifications are required for this interplay and identified TAK1 as a crucial mediator of IL1beta-induced TGFbeta signal modulation. PMID: 28943409
  7. increased TAK1 expression may be involved in the progression of gastric cancer. PMID: 28714004
  8. miR-146a, serving as a tumor suppressor, may significantly promote GC cell apoptosis by inhibition of the NF-kappaB signaling pathway via targeting TAK1. PMID: 28560435
  9. In conclusion, for the first time, we report that TRADD, TRAF2, RIP1 and TAK1 play a role in the regulating TNF-alpha signalling in human myometrium. These findings are of significance given the central role of TNF-alpha in the processes of human labour and delivery. PMID: 28337828
  10. Rab1 is regulated by the host in a similar fashion, and that the innate immunity kinase TAK1 and Legionella effectors compete to regulate Rab1 by switch II modifications during infection. PMID: 27482120
  11. nMet accelerated HCC tumorigenesis and metastasis via the activation of TAK1/NF-kappaB pathway. PMID: 28989054
  12. TAK1 protein expression increased in cartilage tissue from spinal tuberculosis patients. PMID: 28829887
  13. TAK1 regulates Nrf2 through modulation of Keap-p62/SQSTM1 interaction. This regulation is important for homeostatic antioxidant protection in the intestinal epithelium. PMID: 27245349
  14. Overexpression of TAK1 was strongly associated with positive lymph node metastasis in pancreatic ductal adenocarcinoma. PMID: 28194669
  15. dysregulation of the TAK1 complex produces a close phenocopy of Frontometaphyseal Dysplasia caused by FLNA mutations; furthermore, the pathogenesis of some of the filaminopathies caused by FLNA mutations might be mediated by misregulation of signaling coordinated through the TAK1 signaling complex PMID: 27426733
  16. although TAK1 is located at the crossroad of inflammation, immunity, and cancer, this study reports MAP3K7 mutations in a developmental disorder affecting mainly cartilage, bone, and heart PMID: 27426734
  17. This study suggests that aberrant activity of TAK1 impairs autophagy and subsequently leads to alterations in the vitality of retinal pigment epithelial cells. PMID: 26928052
  18. TAK1 may be an important factor involved in the pathogenesis of thyroid cancer, and targeted down-regulation of TAK1 may improve the prognosis of patients with thyroid cancer. PMID: 26823762
  19. Loss of MAP3K7 are associated with esophageal squamous cell carcinoma. PMID: 26406417
  20. This paper highlights that targeting the BMP and TGFbeta type I and type II receptors causes a downregulation of XIAP, TAK1, and Id1 leading to cell death of lung cancer cells. PMID: 27048361
  21. Polyubiquitination of Transforming Growth Factor beta-activated Kinase 1 (TAK1) at Lysine 562 Residue Regulates TLR4-mediated JNK and p38 MAPK Activation PMID: 26189595
  22. The data emphasize the central role of TAK1 in controlling signaling cascades and functional responses in primary neutrophils, making it a promising target for therapeutic intervention in view of the role of neutrophils in chronic inflammatory conditions. PMID: 26491199
  23. MiR-377 is an important negative regulator of E2F and MAP3K7/NF-kB signaling pathway in melanoma cells. PMID: 25889255
  24. the TAK1 signaling pathway may represent a suitable target to design new, antifibrotic therapies PMID: 26185333
  25. Findings indicate that SHIP2 is a regulator of lymphatic function in humans and that inherited mutations in the INPPL1 gene may act in concert with HGF, and likely MAP3K7, mutations to exacerbate lymphatic phenotypes. PMID: 25383712
  26. Data indicate that inhibition of TGF-beta-activated protein kinase 1 (TAK1) reduces chemokine (C-C motif) receptor 7 (CCR7) expression. PMID: 25557171
  27. identify coordinate loss of MAP3K7 and CHD1 as a unique driver of aggressive prostate cancer development PMID: 25770290
  28. Data indicate 4-substituted 1H-pyrrolo[2,3-b]pyridines as potent inhibitors against TGFbeta-activated kinase 1 (TAK1) and mitogen-activated protein kinase kinase kinase kinase 2 (MAP4K2). PMID: 25075558
  29. Ubc13 was dispensable for transforming growth factor beta (TGFbeta)-induced SMAD activation but was required for activation of non-SMAD signaling via TGFbeta-activating kinase 1 (TAK1) and p38 PMID: 25189770
  30. Data show that the ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) complex, including MEKK7 (TAK1) and TNF receptor-associated factor 6 (TRAF6), plays a role in Toll-like receptor 4 -mediated signals to activate NF-kappa B. PMID: 25371197
  31. Data suggest a role for the mitogen-activated protein kinase kinase kinase 7 TAK1-jun-NH2-Terminal Kinase JNK pathway as a critical regulator of NLRP3 protein inflammasome activation. PMID: 25288801
  32. Nef markedly activated TAK1 in M-CSF-derived M2-MPhi but not in GM-CSF-derived M1-MPhi. PMID: 24874739
  33. TAK1 may be an important oncogene or an effective target for renal cell carcinoma intervention. PMID: 25261726
  34. TAK1 plays a role in tumor initiation, progression, and metastasis as a tumor prompter or tumor suppressor. An understanding of the role of TAK1 in liver physiology and diseases is required for the development of therapeutic agencies targeting TAK1 PMID: 24443058
  35. Data suggest TAK1 and IKKbeta (inhibitor of kappaB kinase beta) phosphorylate different serines of IKKbeta; TAK1-catalyzed phosphorylation of IKKbeta at Ser177 is priming event that enables IKKbeta to activate itself by phosphorylating Ser181. PMID: 24911653
  36. NLK functions as a pivotal negative regulator of NF-kappaB via disrupting the interaction of TAK1 with IKKbeta. PMID: 24721172
  37. Data indicate that ribosomal S6 kinase 1 (S6K1) is negatively involved in the toll-like receptorS TLR2 and TLR4 signaling pathway by the inhibition of TAK1 (MAP3K7) activity. PMID: 24277938
  38. A dysregulated balance in the activation of TGFbeta-TAK1 and TGFbeta-SMAD pathways is pivotal for TGFbeta1-induced epithelial-mesenchymal transition. PMID: 24113182
  39. Overexpression of TAK1 predicts a poor prognosis in patients with clear cell renal cell carcinoma, so that TAK1 may serve as a novel prognostic marker PMID: 23534745
  40. our study identifies MAP3K7 deletion as a prominent feature in ERG-negative prostate cancer PMID: 23370768
  41. Results establish TAK1 as an AMPKalpha1 kinase that regulates vascular endothelial growth factor-induced and cytokine-induced angiogenesis by modulating SOD2 expression and the superoxide anion:hydrogen peroxide balance. PMID: 24072697
  42. TAK1 (MAP3K7) does not mediate the TGFb-induced phosphorylation of p38 mitogen-activated protein kinases. PMID: 23760366
  43. 14-3-3epsilon associates with TAK1 in a phosphorylation-dependent manner to determine the cell fate of Bleomycin-treated HCC cells PMID: 23472066
  44. Two SNPs, rs282070 located in intron 1 of the MAP3K7 gene, and rs2111699 located in intron 1 of the GSTZ1 gene, were significantly associated (after adjustment for multiple testing) with longevity in stage 2 PMID: 22576335
  45. Results indicate that TAK1 and p38 kinases appear to be central in the 'priming effect' of LTB(4) on neutrophils to enhance response to Toll-like receptor ligands. PMID: 22843747
  46. findings suggest that DUSP14 negatively regulates TNF- or IL-1-induced NF-kappaB activation by dephosphorylating TAK1 at Thr-187 PMID: 23229544
  47. TAK1 expression correlates with lymph node metastasis and is a negative, independent prognostic factor in resected T3N1-3M0 ESCCs. PMID: 23272845
  48. TAK1 plays central role in both innate and adaptive immunity as well as in DNA damage, osmotic stress, and hypoxia. (Review) PMID: 22941947
  49. We found that endothelial TAK1 and TAB2, but not TAB1, were critically involved in vascular formation PMID: 22972987
  50. review focuses on current insights into the mechanism and function of the Smad-independent signaling pathway via TGF-beta-activated kinase 1 and its role in mediating the profibrotic effects of TGF-beta1 in chronic kidney disease. [Review Article] PMID: 22835455
  51. The phosphorylated form of TAK1 is abundantly expressed in a panel of lymphoma cell lines, including mantle cell, anaplastic large cell, and Hodgkin lymphoma. siRNA inhibited the activation of NF-kappaB and p38 and induced apoptosis in lymphoma cell lines. PMID: 22649101
  52. evidence that TAK1 is a target for YopJ-mediated inhibition. PMID: 22802624
  53. Kaposi's sarcoma associated herpesvirus encoded viral FLICE inhibitory protein K13 activates NF-kappaB pathway independent of TRAF6, TAK1 and LUBAC PMID: 22590573
  54. the role of TAK1 in cell death, cell cycle regulation and apoptosis after X irradiation is independent of NF-kappaB, p38 MAPK, and ERK phosphorylation, and dependent, in part, on p21 induction PMID: 22490020
  55. Inhibition of transforming growth factor-activated kinase 1 (TAK1) blocks and reverses epithelial to mesenchymal transition of mesothelial cells PMID: 22384029
  56. In the grafts containing Tak1-suppressed prostate stem cells, p38 and c-jun-NH(2)-kinase activity was attenuated and proliferation was increased PMID: 22467172
  57. TAK1 activity was required for HIV-1 gp41 cytoplasmic domain-mediated NF-kappaB activation, and HIV-1-derived gp41 cytoplasmic domain physically interacted with TAK1 through the same region required for NF-kappaB activation. PMID: 22341466
  58. polyubiquitination of TAK1 is correlated with activation of TAK1 and is essential for activation of NF-kappaB signaling downstream of several receptors PMID: 22069318
  59. Data suggest that TAK1 functions as a prosurvival mediator in cancer cells displaying hyperactive KRAS-dependent Wnt signaling. PMID: 22341439
  60. TAK1 and its adapter protein, TAB2, reciprocally regulate both TAK1- and ASK1-mediated signaling pathways to direct the activations of NF-kappaB and AP-1. PMID: 22167179
  61. Study on effect of fushenkeli on expression of TAK1 in human renal tubular epithelial cells and its possible mechanism PMID: 22256769
  62. TAK1 plays a major role in growth factor-induced phenotypic modulation of airway smooth muscle remodeling. PMID: 21873447
  63. these studies show that the TAK1-TAB2-TAB3 signaling axis is critical for carcinoma-induced bone lesions, mediating expression of proinvasive and osteolytic factors. PMID: 21700681
  64. Data show that DNA damage stimulates the formation of a cytosolic complex of ATM, NEMO, RIP1, and TAK1, and that TAK1 mediates the NF-kappaB and p38 mitogen-activated protein kinase (MAPK)/MAPK-activated protein 2 responses to DNA damage. PMID: 21606198
  65. The TAK1 regulated inhibition of TNF-alpha-mediated apoptosis via the autocrine action of stem cell factor. PMID: 21233843
  66. Results that genetic silencing or inhibition of TAK1 kinase activity in vivo is a potential therapeutic approach to reversal of the intrinsic chemoresistance of pancreatic cancer. PMID: 21743023
  67. TAK1-c-Rel and IRF4 pathways play distinct roles in the maintenance of IL-9-producing Th17 phenotype of HTLV-1-transformed cells PMID: 21498517
  68. p38 maintains E-cadherin expression by suppressing TAK1-NF-kappaB signaling, thus impeding the induction of epithelial-to-mesenchymal transition in human primary mesothelial cells. PMID: 21098640
  69. LPS-induced activation of IRAK4 and TAK1, K63-linked polyubiquitination of IRAK1 and TRAF6, and disrupted IRAK1-TRAF6 and IRAK1-IKKgamma assembly associated with increased A20 expression PMID: 21220427
  70. TAK1 lysine 158, but not lysine 34, is required for TGF-beta-induced TAK1 polyubiquitination and TGF-beta-induced TRAF6-mediated Smad-independent IKK, JNK and p38 activation. PMID: 20837137
  71. The systemic TAK1 gene silencing decreases the frequency of Th1 and Th17 cells, both mediating autoimmunity in experimental arthritis, demonstrating the immunomodulatory potential of TAK1. PMID: 20682854
  72. ATM- and NEMO-dependent ubiquitination of ELKS leads to the ubiquitin-dependent assembly of TAK1/TAB2/3 and NEMO/IKK complexes, resulting in IKK and NF-kappaB activation following genotoxic stimuli. PMID: 20932476
  73. Co-expression of TAK1 and TAB1 resulted in a functional and active TAK1-TAB1 complex capable of directly activating full-length heterotrimeric mammalian AMP-activated protein kinase (AMPK) in vitro. PMID: 20538596
  74. A20 improves cardiac functions and inhibits cardiac hypertrophy, inflammation, apoptosis, and fibrosis by blocking transforming growth factor-beta-activated kinase 1-dependent signaling. PMID: 20585109
  75. findings provide the first evidence that TAK1-NLK pathway is a novel regulator of FOXO1 PMID: 20061393
  76. Lys(63)-linked polyubiquitination of TAK1 at Lys(158) is essential for its own kinase activation and its ability to mediate its downstream signal transduction pathways in response to TNFalpha and IL-1beta stimulation. PMID: 20038579
  77. polyubiquitination of TAK1 regulates the activation of NF-kappaB, which in turn is used by H. pylori CagA for the H. pylori-induced inflammatory response PMID: 19820695
  78. Interleukin-1 (IL-1) receptor-associated kinase-dependent IL-1-induced signaling complexes phosphorylate TAK1 and TAB2 at the plasma membrane and activate TAK1 in the cytosol. PMID: 12242293
  79. TAK1-dependent activation of AP-1 and c-Jun N-terminal kinase by receptor activator of NF-kappaB. PMID: 12296995
  80. The TAK1-TAB1 fusion protein is a novel constitutively active mitogen-activated protein kinase kinase kinase that stimulates AP-1 and NF-kappaB signaling pathways PMID: 12372426
  81. TNFalpha activation of the NF-kappaB pathway is associated with the inducible binding of TAK1 to TRAF2 and both IKKalpha and IKKbeta PMID: 12547194
  82. TAK1- and MKK3-mediated activation of p38 are facilitated by Smad7 PMID: 12589052
  83. Pellino2 interacts with TAK1 and activates the MAP kinase pathway. PMID: 12804775
  84. TAK1 has a role in TNF-alpha induced IKK phosphorylation of NF-kappaB p65 PMID: 12842894
  85. Results indicate that dominant negative constructs of TAK1 retain the ability to intercept the TGF-beta signaling effectively. PMID: 12859960
  86. TAB1 participates in a SAPK2a/p38alpha-mediated feedback control of TAK1 PMID: 14592977
  87. Tak1 has a role in Wnt signal transduction and phosphorylation and inhibition of TCF PMID: 14960582
  88. TAK1 protein kinase mediate IKK activation by BCL10 and MALT1. RNAi-mediated silencing of TAK1 suppressed TCR-dependent IKK activation and interleukin-2 production in T cells PMID: 15125833
  89. TAK1 binds with Sef, which has a role in JNK activation and apoptosis PMID: 15277532
  90. characterized the molecular mechanisms of cellular stress-induced TAK1 activation, focusing mainly on the phosphorylation of TAK1 at Thr-187 and Ser-192 in the activation loop; TAB1 and TAB2 were differentially involved in the phosphorylation of TAK1 PMID: 15590691
  91. Experiments using dominant-negative Tpl2 suggest this kinase functions distal to TRAFs but proximal to the TAK1/TAB1 signaling complex, within the IKK/NFkappaB activation pathway. PMID: 15670770
  92. interleukin-1beta and its downstream mediator TAK1 inhibit mothers against decapentaplegic homolog 3 MAD-mediated TGFbeta target gene activation PMID: 15917296
  93. Arachidonic acid-mediated TAK1 activation is responsible for MAP kinase kinase 6 activation and the ensuing downstream signaling in metastatic human breast carcinoma cells PMID: 16000313
  94. Involvement of the TAB2/TRAF6/TAK1 signalling complex in the Edar signal transduction pathway has important implications for understanding of NF-kappaB activation and anhidrotic ectodermal dysplasia in human. PMID: 16251197
  95. TAK1 is recruited to the TNF-R1 complex via RIP and likely cooperates with MEKK3 to activate NF-kappaB in TNF-alpha signaling PMID: 16260783
  96. LMP1 utilizes two distinct pathways to activate NF-kappaB: a major one through CTAR2/TRAF6/TAK1/IKKbeta (canonical pathway) and a minor one through CTAR1/TRAF3/NIK/IKKalpha (noncanonical pathway) PMID: 16280329
  97. TAK1 plays a critical role in T cell activation by controlling production of IL-2. PMID: 16293250
  98. an LMP1-associated complex containing TRAF6, TAB2, and TAK1 plays an essential role in the activation of JNK PMID: 16446357
  99. A candidate gene in ectodermal dysplasia. PMID: 16527194
  100. TAK1 is a functional member of the Snf1/AMPK kinase family PMID: 16835226

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Involvement in disease Frontometaphyseal dysplasia 2 (FMD2); Cardiospondylocarpofacial syndrome (CSCF)
Subcellular Location Cytoplasm, Cell membrane, Peripheral membrane protein, Cytoplasmic side
Protein Families Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily
Tissue Specificity Isoform 1A is the most abundant in ovary, skeletal muscle, spleen and blood mononuclear cells. Isoform 1B is highly expressed in brain, kidney and small intestine. Isoform 1C is the major form in prostate. Isoform 1D is the less abundant form.
Database Links

HGNC: 6859

OMIM: 157800

KEGG: hsa:6885

STRING: 9606.ENSP00000358335

UniGene: Hs.594838

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