MOB1A Antibody

Datasheet

Code	CSB-PA872519HA01HU
Size	US$166
	Order now
Image	Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA872519HA01HU at dilution of 1:100 Western Blot Positive WB detected in: K562 whole cell lysate, Jurkat whole cell lysate All lanes: MOB1A antibody at 3.3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 26, 17 kDa Observed band size: 26 kDa IHC image of CSB-PA872519HA01HU diluted at 1:1000 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA872519HA01HU at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunoprecipitating MOB1A in Jurkat whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA872519HA01HU in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA872519HA01HU (5µg) + Jurkat whole cell lysate (500µg) Lane 3: Jurkat whole cell lysate (20µg)
The Latest Promotion
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MOB1A Polyclonal antibody

Uniprot No.

Q9H8S9

Target Names

MOB1A

Alternative Names

B1; Mps One Binder kinase activator like 1B (yeast) antibody; C2orf6 antibody; Chromosome 2 open reading frame 6 antibody; FLJ10788 antibody; FLJ11595 antibody; MATS1 antibody; MOB kinase activator 1A antibody; MOB1 antibody; Mob1 alpha antibody; Mob1 homolog 1B antibody; MOB1 Mps One Binder homolog A antibody; MOB1 Mps One Binder kinase activator like 1B antibody; Mob1A antibody; MOB4B antibody; Mob4B protein antibody; MOBK1B antibody; Mobkl1b antibody; MOL1B_HUMAN antibody; Mps one binder kinase activator like 1B antibody; Mps one binder kinase activator-like 1B antibody; Protein Mob4B antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human MOB kinase activator 1A protein (2-216AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MOB1A Antibody (Product code: CSB-PA872519HA01HU) is Non-conjugated. For MOB1A Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA872519HB01HU	MOB1A Antibody, HRP conjugated	ELISA
FITC	CSB-PA872519HC01HU	MOB1A Antibody, FITC conjugated
Biotin	CSB-PA872519HD01HU	MOB1A Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:2000-1:5000
IHC	1:100-1:1000
IF	1:200-1:500
IP	IP1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38 and STK38L. Acts cooperatively with STK3/MST2 to activate STK38.

Gene References into Functions

validated the functional importance of an identified interaction network by characterizing a distinct novel interaction between PTPN5 and Mob1a. PMID: 28675297
Through a comprehensive set of biochemical, biophysical, mutational and structural studies, we quantitatively assess how phosphorylation of MOB1A regulates its interaction with both MST kinases and LATS/NDR family kinases in vitro. PMID: 28373297
In this study, we investigated the mechanisms behind the recruitment of MST1 and MST2 kinases to MOB1, which facilitate signal transmission in the Hippo pathway by bringing the MST1 and MST2 kinases in close vicinity to their substrates, the LATS family kinases. PMID: 28373298
MOB1 binds differently to the NDR2 and LATS1 kinases.MOB1 interaction with Hippo and MST protein is not essential for development and tissue growth control. PMID: 28947795
No relationship has been found between the MOBKL1B-NS5A interaction and hepatitis C virus replication. PMID: 25031347
The RING ligase praja2 ubiquitylates and degrades Mob, a core component of NDR/LATS kinase and a positive regulator of the tumour-suppressor Hippo cascade. PMID: 23652010
Mob1A and Mob1B are needed for cell abscission and centriole re-joining after telophase and cytokinesis. PMID: 22454515
Results suggest that Mob1 and the other mammalian orthologues of the mitotic exit network regulate mitotic progression by facilitating the timely mobilization of the chromosomal passenger complex to the spindle midzone. PMID: 19955215
hMOB1A and hMOB1B are 2 LATS-binding proteins that may function as tumor suppressors in human cancer cells. PMID: 19739119
Mats1 can rescue the lethality associated with loss of Mats function in Drosophila; As Mats1 is mutated in human tumors, Mats-mediated growth inhibition and tumor suppression is likely conserved in humans PMID: 15766530
MATS1 mRNA expression is suppressed in tumor tissue and its low expression is associated with tumor growth, invasion and metastasis of colorectal cancer PMID: 17611689

Hide All

Protein Families

MOB1/phocein family

Tissue Specificity

Adrenal gland, bone marrow, brain, placenta, prostate, salivary gland, skeletal muscle, testis, thymus, thyroid gland, heart, spinal cord, fetal brain and fetal liver.

Database Links

HGNC: 16015

OMIM: 609281

KEGG: hsa:55233

STRING: 9606.ENSP00000379364

UniGene: Hs.602092

Promotions

Transmembrane Protein Questionnaire with Gifts

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

The Overview of Hippo Signaling Pathway Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Hippo signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address