NDUFAF2 Antibody

Datasheet

Code	CSB-PA836643LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, PC-3 whole cell lysate All lanes: NDUFAF2 antibody at 2.7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 20 kDa Observed band size: 20 kDa IHC image of CSB-PA836643LA01HU diluted at 1:500 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA836643LA01HU diluted at 1:500 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NDUFAF2 Polyclonal antibody

Uniprot No.

Q8N183

Target Names

NDUFAF2

Alternative Names

B17.2 like antibody; B17.2-like antibody; B17.2L antibody; FLJ22398 antibody; MIMIT_HUMAN antibody; Mimitin antibody; Mimitin mitochondrial antibody; mitochondrial antibody; MMTN antibody; Myc induced mitochondrial protein antibody; Myc-induced mitochondrial protein antibody; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 2 antibody; NADH dehydrogenase (ubiquinone) complex I; assembly factor 2 antibody; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2 antibody; NDUFA12 like antibody; NDUFA12 like protein antibody; NDUFA12-like protein antibody; NDUFA12L antibody; NDUFAF2 antibody; OTTHUMP00000161882 antibody; OTTHUMP00000221703 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2 protein (117-169AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The NDUFAF2 Antibody (Product code: CSB-PA836643LA01HU) is Non-conjugated. For NDUFAF2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA836643LB01HU	NDUFAF2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA836643LC01HU	NDUFAF2 Antibody, FITC conjugated
Biotin	CSB-PA836643LD01HU	NDUFAF2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

Acts as a molecular chaperone for mitochondrial complex I assembly. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Gene References into Functions

One patient presented with Leigh syndrome and had a homozygous deletion in the NDUFAF2 gene, while the second patient had a homozygous mutation in the POLG gene, [c.1399G>A; p.Ala467Thr]. PMID: 27344355
Mimitin and 14-3-3 protein zeta/delta are potential markers of paclitaxel resistance and prognostic factors in ovarian cancer. PMID: 26033570
Within the clinical spectrum of Leigh disease, patients with mutations in NDUFAF2 present with a distinct clinical pattern with predominantly brainstem involvement on MRI PMID: 20571988
the novel gene mimitin is a direct transcriptional target of c-Myc, and is involved in Myc-dependent cell proliferation in esophageal squamous cell carcinoma cells PMID: 15774466
B17.2L occurred in a 830 kDa subcomplex specifically in patients with mutations in subunits NDUFV1 and NDUFS4 PMID: 17383918
the homozygous substitution in NDUFAF2 is the disease-causing mutation, which results in a complex I deficiency in the fibroblasts PMID: 19384974

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Involvement in disease

Mitochondrial complex I deficiency (MT-C1D); Leigh syndrome (LS)

Subcellular Location

Mitochondrion.

Protein Families

Complex I NDUFA12 subunit family

Tissue Specificity

Highly expressed in ESCC cells. Also expressed in heart, skeletal muscle, liver, and in fibroblasts.

Database Links

HGNC: 28086

OMIM: 252010

KEGG: hsa:91942

STRING: 9606.ENSP00000296597

UniGene: Hs.591757

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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