Name: PHGDH Antibody
Brand: CUSABIO
SKU: CSB-PA03255A0Rb

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PHGDH Polyclonal antibody

Uniprot No.

O43175

Target Names

PHGDH

Alternative Names

3 PGDH antibody; 3-PGDH antibody; 3-phosphoglycerate dehydrogenase antibody; 3PGDH antibody; D-3-phosphoglycerate dehydrogenase antibody; EC 1.1.1.95 antibody; Epididymis secretory protein Li 113 antibody; HEL S 113 antibody; NLS antibody; NLS1 antibody; PDG antibody; PGAD antibody; PGD antibody; PGDH antibody; PGDH3 antibody; Phgdh antibody; PHGDHD antibody; Phosphoglycerate dehydrogenase antibody; SERA antibody; SERA_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human D-3-phosphoglycerate dehydrogenase protein (2-251AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function	Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate.
Gene References into Functions	PHGDH expression is regulated by PlncRNA-1 in breast cancer. PMID: 29626321 Study provides evidence that a unique metabolic program is activated in a lung adenocarcinoma subset, described by PHGDH, which confers cell growth. PMID: 28614715 Data indicate that the expression of PHGDH is increased in pancreatic cancer and is an independent molecular prognostic factor for pancreatic cancer patients. In addition, PHGDH controls cell proliferation, migration and invasion abilities. PMID: 29128633 Data show there was a significant negative correlation between PHGDH copy-number alteration and EPAS1 (HIF2A) expression. PMID: 28951458 Therefore, we show for the first time that the nuclear localization of Cat L and its substrate Cux1can be positively regulated by Snail NLS and importin beta1, suggesting that Snail, Cat L and Cux1 all utilize importin beta1 for nuclear import. PMID: 28698143 High PHGDH expression is associated with idiopathic pulmonary fibrosis. PMID: 27836973 This report present 6 individuals from 3 unrelated families with infantile serine biosynthesis defect due to PGDH deficiency. PMID: 28135894 Overexpression of Phgdh may be generally associated with CK5 cells, and oncogenic function may be determined by isoform expression. PMID: 26026368 High expression of PHGDH is associated with Colon Cancer. PMID: 26439504 p53-mediated repression of PHGDH enhances the apoptotic response upon serine starvation in melanoma cells. PMID: 25404730 Phosphoglycerate Dehydrogenase deficiency is associated with Neu-Laxova syndrome. PMID: 25152457 We report on the identification of homozygous mutations in PHGDH and serine deficiency in individuals with Neu-Laxova syndrome. This disorder thus seems to be an extremely severe expression of PHGDH deficiency. PMID: 24836451 PHGDH overexpression is found in cervical cancer, in particular, in bigger tumors and with advanced stages. Its expression is positively correlated with squamous cell carcinoma antigen level PMID: 24247658 The potential mechanisms by which PHGDH promotes cancer are discussed. PMID: 21981974 in some cancer cells a relatively large amount of glycolytic carbon is diverted into serine and glycine metabolism through phosphoglycerate dehydrogenase. PMID: 21804546 results reveal that certain breast cancers are dependent upon increased serine pathway flux caused by PHGDH overexpression and demonstrate the utility of in vivo negative-selection RNAi screens for finding potential anticancer targets PMID: 21760589 Studies in bacteria showed that addition of substrate at the active site is ordered, with HPAP binding before NADH. Also, NADH can compete with the substrate for binding to the allosteric site and thereby eliminate the substrate inhibition. PMID: 19388702 The crystal structure of Mycobacterium tuberculosis D-3-phosphoglycerate dehydrogenase has been solved with bound effector, 1-serine, and substrate, hydroxypyruvic acid phosphate. The human enzyme was also examined. PMID: 18627175 The frequency of antibodies to Phgdh is much higher in patients with autoimmune hepatitis than in patients with other types of hepatitis or normal controls. PMID: 19497206 PHGDH is expressed in cytoplasm of stromal and glandular cells in endometrium; expression is relatively high in proliferative phase and lower in secretory phase. Data suggest expression of PHGDH in endometrium is regulated by HOXA10. PMID: 19778996 we conclude that this mutation impairs the folding and/or assembly of PHGDH but has minimal effects on the activity or stability of that portion of the V490M mutant that reaches a mature conformation PMID: 11751922 These data suggest that missense mutations associated with 3-PGDH deficiency either primarily affect substrate binding or result in very low residual enzymatic activity. PMID: 19235232 a coding PHGDH SNP (rs543703) was weakly associated with the development of schizophrenia in Korean population PMID: 19404161 Show More Hide All
Involvement in disease	Phosphoglycerate dehydrogenase deficiency (PHGDHD); Neu-Laxova syndrome 1 (NLS1)
Protein Families	D-isomer specific 2-hydroxyacid dehydrogenase family
Database Links	HGNC: 8923 OMIM: 256520 KEGG: hsa:26227 STRING: 9606.ENSP00000358417 UniGene: Hs.487296

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Check Code：

Code	CSB-PA03255A0Rb
Size	US$299Purchase it in Cusabio online store (only available for customers from the US)
Image	Western blot All lanes: PHGDH antibody at 2µg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA03255A0Rb at dilution of 1:100 Immunofluorescent analysis of MCF-7 cells using CSB-PA03255A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) Immunofluorescent analysis of PC-3 cells using CSB-PA03255A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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