PLA2G4C Antibody

Datasheet

Code	CSB-PA892485ESR1HU
Size	US$166
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Image	Western Blot Positive WB detected in: A549 whole cell lysate(30µg), MCF7 whole cell lysate(30µg), U251 whole cell lysate(320µg), HepG2 whole cell lysate(30µg), SHSY5Y whole cell lysate(30µg), Mouse Heart tissue lysate30µg),Mouse Kidney tissue lysate30µg),Mouse skeletal muscle tissue lysate30µg),Rat Heart tissue lysate30µg) All lanes: PLA2G4C antibody at 1:500 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 61,60,63 kDa Observed band size: 70 kDa Exposure time: 120s IHC image of CSB-PA892485ESR1HU diluted at 1:50 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody Immunofluorescence staining of A549 cell with CSB-PA892485ESR1HU at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of U251 cell with CSB-PA892485ESR1HU at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of U251 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PLA2G4C Polyclonal antibody

Uniprot No.

Q9UP65

Target Names

PLA2G4C

Alternative Names

cPLA2 gamma antibody; Cytosolic phospholipase A2 gamma antibody; EC 3.1.1.4 antibody; PA24C_HUMAN antibody; Phospholipase A2 group IVC antibody; PLA2G4C antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

synthesized peptide derived from human PLA2G4C

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:100
IF	1:20-1:100

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Calcium-independent phospholipase, lysophospholipase and O-acyltransferase involved in phospholipid remodeling with implications in endoplasmic reticulum membrane homeostasis and lipid droplet biogenesis. Preferentially hydrolyzes the ester bond of the fatty acyl group attached at the sn-2 position of phospholipids with choline and ethanolamine head groups, producing lysophospholipids that are used in deacylation-reacylation cycles. Transfers the sn-1 fatty acyl from one lysophospholipid molecule to the sn-2 position of another lysophospholipid to form diacyl, alkylacyl and alkenylacyl glycerophospholipids. Cleaves ester bonds but not alkyl or alkenyl ether bonds at sn-1 position of lysophospholipids. Catalyzes sn-2 fatty acyl transfer from phospholipids to the sn-2 position of 1-O-alkyl or 1-O-alkenyl lysophospholipids with lower efficiency. In response to dietary fatty acids, may play a role in the formation of nascent lipid droplets from the endoplasmic reticulum likely by regulating the phospholipid composition of these organelles.; (Microbial infection) May play a role in replication and assembly of human hepatitis C virus (HCV). In response to HCV infection, promotes remodeling of host endoplasmic reticulum membranes to form organelle-like structures called membranous web, where HCV replication occur. Can further mediate translocation of replication complexes to lipid droplets to enable virion assembly.; (Microbial infection) May facilitate human T-lymphotropic virus type 1 (HTLV-1) infection by promoting leukotriene B4 (LTB4) biosynthesis. LTB4 acts as a chemoattractant for HTLV-1-infected CD4-positive T cells and favors cell to cell viral transmission.

Gene References into Functions

Five single-nucleotide polymorphisms (SNPs) (rs9226, rs1045376, rs251684, rs2307279, and rs156631) in PLA2G4C and four SNPs (rs6533451, rs2285714, rs2285713, and rs11728699) in PLA2G12A were selected and genotyped. Our results showed a significant association between ASD and the rs251684 variant of PLA2G4C. The rs251684 polymorphism of PLA2G4C may be associated with Autism Spectrum Disorder risk. PMID: 27611910
The A allele in PLA2G4C single nucleotide polymorphism (rs1549637) is associated with a worse prognosis in patients with colorectal cancer, especially in stage II. PMID: 26364726
site-specific cytosine methylation of the CpT of PLA2G4C may play a role in the pathogenesis of schizophrenia. PMID: 22878031
These findings suggest that IVC-PLA(2) is involved in regulations of macrophage differentiation and macrophage polarization. PMID: 22108055
these results suggest that cPLA(2)gamma plays an important role in cancer cell chemotaxis. PMID: 21600875
Findings suggest that variation in PLA2G4C may influence preterm birth risk by increasing levels of prostaglandins, which are known to regulate labor. PMID: 21184677
cPLA(2) has a role in the induction of cell autonomous cellular immunity to Chlamydia PMID: 20452986
locus showed a weak association with schizophrenia in a Chinese population PMID: 20016400
Novel PLA2G4C polymorphism as a molecular diagnostic A minisatellite polymorphism in the untranslated region of exon 1, with allelic variants that have one, two or three 27-bp repeats PMID: 11958371
enzymatic properties PMID: 12039969
TNF-alpha & IFN-gamma induce gene expression of a novel cytosolic PLA(2) IVC in human airway epithelial cells. The possibility that it is involved in cytokine-mediated inflammation in the respiratory tract is inferred. PMID: 12396716
Down-regulation of Phospholipase A2 is associated with colon tumorigenesis PMID: 15788676
identification of its remarkable substrate selectivity that results in the highly selective generation of 2-arachidonoyl lysophosphatidylcholine PMID: 15908428
results indicate that complete C-terminal processing is important for the functional expression of cPLA2, although not for its membrane localization PMID: 15944408
Caspase-3-dependent activation of calcium-independent phospholipase A2 enhances cell migration in non-apoptotic ovarian cancer cells PMID: 16882668
iPLA2gamma has roles in the lipid plasticity of myocardium, in generating signaling metabolites, and in modulating energy storage and utilization in myocardium in different metabolic contexts PMID: 17213206
Single nucleotide polymorphism is asociated with schizzophrenia in a Chinese population. PMID: 17417066
endometrial cell PGE2 biosynthesis was associated with PLA2G4C expression and activity PMID: 17459165
The subcellular localization and enzymatic properties of cPLA2gamma with C-terminal FLAG-tag, was examined. PMID: 19501189

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Subcellular Location

Cell membrane; Lipid-anchor. Endoplasmic reticulum membrane; Lipid-anchor. Mitochondrion membrane; Lipid-anchor. Lipid droplet.

Tissue Specificity

Highly expressed in heart and skeletal muscle.

Database Links

HGNC: 9037

OMIM: 603602

KEGG: hsa:8605

STRING: 9606.ENSP00000400036

UniGene: Hs.631562

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

MAPK signaling pathway VEGF signaling pathway Vascular smooth muscle contraction Necroptosis Ras signaling pathway Fc epsilon RI signaling pathway Oxidative Phosphorylation Phospholipase D signaling pathway Glutamatergic synapse

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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