PRSS16 Antibody

Datasheet

Code	CSB-PA882073LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Rat liver tissue, Mouse liver tissue All lanes: PRSS16 antibody at 3.2μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56 kDa Observed band size: 56 kDa IHC image of CSB-PA882073LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA882073LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
Citations
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Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PRSS16 Polyclonal antibody

Uniprot No.

Q9NQE7

Target Names

PRSS16

Alternative Names

PRSS16 antibody; TSSP antibody; Thymus-specific serine protease antibody; EC 3.4.-.- antibody; Serine protease 16 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human Thymus-specific serine protease protein (352-489AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PRSS16 Antibody (Product code: CSB-PA882073LA01HU) is Non-conjugated. For PRSS16 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA882073LB01HU	PRSS16 Antibody, HRP conjugated	ELISA
FITC	CSB-PA882073LC01HU	PRSS16 Antibody, FITC conjugated
Biotin	CSB-PA882073LD01HU	PRSS16 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

The clinicopathological significance of thymic epithelial markers expression in thymoma and thymic carcinoma H Li,BMC cancer,2023

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : Immunohistochemical staining

Sample type: cell

Review: For immunohistochemical analysis, the antibodies employed included PRSS16, cathepsin V, claudin-4, CD40. Expression of cortical epithelial markers in thymoma and TSCC.A network of PRSS16-positive epithelial cells was seen in B2.

By Anonymous

Target Background

Function

Protease that may play a role in T-cell development.

Gene References into Functions

the level of TSSP expression by thymic dendritic cells may modify the risk factors for multiple sclerosis conferred by some MHC class II haplotypes PMID: 29061767
The genes HIST1H2BJ, PRSS16, and PGBD1 were not associated with Japanese patients with schizophrenia. PMID: 22488895
ZNF804a regulates expression of the schizophrenia-associated genes PRSS16, COMT, PDE4B, and DRD2 PMID: 22384243
The gene encoding thymus-specific serine protease (PRSS16) maps to the extended HLA complex, which harbours several genes predisposing for autoimmune diseases. PMID: 12140752
data suggest that it is unlikely that polymorphisms within the PRSS16 gene are involved in the predisposition to diabetes type 1 PMID: 17584581
our results identify PRSS16 and BTN3A2, two genes thought to play important roles in regulating the immune response, as potentially novel susceptibility genes for Type I diabetes. PMID: 19295542

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Subcellular Location

Cytoplasmic vesicle. Note=Vesicular, either lysosomal or endosomal.

Protein Families

Peptidase S28 family

Tissue Specificity

Expressed predominantly in cortical thymic epithelial cells.

Database Links

HGNC: 9480

OMIM: 607169

KEGG: hsa:10279

STRING: 9606.ENSP00000230582

UniGene: Hs.274407

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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