PTEN (Ab-370) Antibody

Code CSB-PA000679
Size US$297
  • Western blot analysis of extracts from C2C12 cells using PTEN(Ab-370) Antibody.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PTEN(Ab-370) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) PTEN Polyclonal antibody
Uniprot No. P60484
Target Names PTEN
Alternative Names 10q23del antibody; BZS antibody; DEC antibody; GLM2 antibody; MGC11227 antibody; MHAM antibody; MMAC1 antibody; MMAC1 phosphatase and tensin homolog deleted on chromosome 10 antibody; Mutated in multiple advanced cancers 1 antibody; Phosphatase and tensin homolog antibody; Phosphatase and tensin like protein antibody; Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN antibody; Pten antibody; PTEN_HUMAN antibody; PTEN1 antibody; TEP1 antibody
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Immunogen Peptide sequence around aa.368~372 (D-V-S-D-N) derived from Human PTEN.
Immunogen Species Homo sapiens (Human)
Clonality Polyclonal
Purification Method Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration It differs from different batches. Please contact us to confirm it.
Form Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Tested Applications ELISA,WB,IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:1000
IHC 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4
Gene References into Functions
  1. Nuclear phosphatase and tensin homologue on chromosome ten protein (PTEN) interacts with the splicing machinery, spliceosome, to regulate its assembly and pre-mRNA splicing. PMID: 29921876
  2. the expression of PTEN and miR-144 was inversely correlated in metastatic breast cancer cell lines. PMID: 30132256
  3. Disruption of PTEN proteinisoform PTENbeta (PTENbeta) alters rDNA transcription and promotes ribosomal biogenesis. PMID: 28332494
  4. Functionally, Ataxin-3 overexpression promoted cell proliferation, and Ataxin-3 knockdown inhibited cell proliferation in testicular cancer cell. In addition, up-regulation of Ataxin-3 inhibited the expression of PTEN and activated the AKT/mTOR pathway. PMID: 29902454
  5. a certain degree of mitochondrial oxidative activity, with some difference for PTEN-wild type SF767 cells respect to PTEN-deleted A172 and U87MG characterized by a loss-of-function point mutation of PTEN. PMID: 29209894
  6. We demonstrated that expression of PTEN and miR-718 were significantly correlated in patients with gastric cancer. Low expression of PTEN and high level of miR-718 were notably associated with a lower 5-year overall survival rate. Both PTEN and miR-718 were identified as prognostic factors of gastric cancer. PMID: 30131483
  7. The data indicate that diagnostic or therapeutic chest radiation may predispose patients with decreased stromal PTEN expression to secondary breast cancer, and that prophylactic EGFR inhibition may reduce this risk. PMID: 30018330
  8. findings indicated that shikonin inhibits proliferation and promotes apoptosis in human endometrioid endometrial cancer (EEC) cells by modulating the miR-106b/PTEN/AKT/mTOR signaling pathway, suggesting shikonin could act a potential therapeutic agent in the EEC treatment. PMID: 29449346
  9. SIRT6 inhibited proliferation, migration, and invasion of colon cancer cells by up-regulating PTEN expression and down-regulating AKT1 expression. PMID: 29957460
  10. Data show that phosphatase and tensin homolog (PTEN) interacts with death domain associated protein (DAXX) and, in turn PTEN directly regulates oncogene expression by modulating DAXX-histone H3.3 (H3.3) association on the chromatin. PMID: 28497778
  11. Study suggested that there may be a regulatory loop between miR21 and PTEN, and that miR21 inhibition affected the proliferative, invasive and apoptotic abilities of oral squamous cell carcinoma (OSCC) cells. miR-21 expression was observed in 80.0% OSCC tissues and in 30.0% of normal tissues. By contrast, PTEN expression exhibited an opposite trend in OSCC tissues 37.1%, and normal tissues 80.0%. PMID: 30132571
  12. MTSS1 is stabilized by the protein phosphatase activity of the tumor suppressor PTEN. Our data show that PTEN loss in PDAC cells results in both increased metastatic potential and decreased MTSS1 expression. Furthermore, we show that ectopic MTSS1 expression rescues this effect. PMID: 29175021
  13. Low PTEN mRNA expression was associated with down-regulation of a group of genes involved in immune responses and B-cell development. PMID: 29734016
  14. Results showed that MiR-374b was highly expressed, while PTEN was downregulated in the GIST tissues. The levels of miR-374b, PI3K, AKT and PTEN were related to tumor diameter and pathological stage. Additionally, miR-374b increased the mRNA and protein levels of PI3K, Akt, MMP2, MMP9, P53 and cyclinD1, suggesting that miR-374b activates PI3K/Akt signaling pathway in GIST-T1 cells. PMID: 29902839
  15. PTEN loss is associated with castration-resistant prostate cancer. PMID: 29302046
  16. Low PTEN expression is associated with thyroid cancer progression. PMID: 30015900
  17. we provide a review on current understandings of the regulation of PTEN by ncRNAs, which could contribute to the development of novel approaches to the diseases with abnormal expression of PTEN. PMID: 30217221
  18. The IRIS-driven metastatic mechanism results from IRIS-dependent suppression of phosphatase and tensin homolog (PTEN) transcription, which in turn perturbs the PI3K/AKT/GSK-3beta pathway leading to prolyl hydroxylase-independent HIF-1alpha stabilization and activation in a normoxic environment. PMID: 30254159
  19. In this study, we used the Ion Personal Genome Machine (PGM) and Ion Torrent Ampliseq Cancer panel to sequence hotspot regions from PIK3CA, AKT and PTEN genes to identify genetic mutations in 39 samples of TNBC subtype from Moroccan patients and to correlate the results with clinical-pathologic data PMID: 30227836
  20. Data indicate a significant prognostic role for assessing transcriptional regulator ERG (ERG) and phosphatase and tensin homolog protein (PTEN) in men with prostate cancer. PMID: 30101374
  21. Low PTEN expression is associated with multiple myeloma. PMID: 30015974
  22. The loss of Sirt3 triggered fatal mitochondrial fission by suppressing the Akt/PTEN pathway. PMID: 30021354
  23. Results showed that SIX1 was overexpressed in osteosarcoma tissues, blood samples and cell lines, whereas PTEN expression was reduced. PMID: 29807230
  24. miR23b3p and PTEN interfered with the viability and apoptosis of smooth muscle cells. PMID: 29845190
  25. PDCD4 and PTEN were the functional targets of miR-21. PMID: 30074182
  26. miR-205 functions as an oncogenic miRNA by directly binding to SMAD4 and PTEN, providing a novel target for the molecular treatment of ovarian cancer. PMID: 28145479
  27. Studies have indicated that in breast cancer, PTEN undergo mutations. There is a functional and mechanistic link between the BMI-1 oncoprotein and tumor suppressor PTEN in the development and progression of breast cancer. [review] PMID: 30096458
  28. When considered together (43 cases), 1/25 cases (4%) with a PIK3CA mutation and/or low PTEN expression levels had a pathologic complete response (pCR)compared to 7/18 cases (39%) with wild-type PI3KCA and high PTEN expression levels (p = 0.006). PMID: 29110152
  29. Taken together, the authors presented here a novel cross-talk between miR-181a and PTEN which was raised by hepatitis B virus X protein, and this shined a new line in hepatitis B virus-related hepato-carcinogenesis. PMID: 28053323
  30. Bioinformatics analysis demonstrated that the 3'UTR of PTEN mRNA was targeted by hsa-miR-142-5p which regulates its expression triggering cancer stem cell-like properties of cutaneous squamous cell carcinoma. PMID: 28857248
  31. PTEN lipid phosphatase inactivation abolished the MOB1-LATS1/2 interaction, decreased YAP phosphorylation and finally promoted YAP nuclear translocation, which enhanced the synergistic effect of YAP-TEAD, thus inducing cell proliferation and migration. PMID: 30134988
  32. TERT could induce thyroid carcinoma cell proliferation mainly through the PTEN/AKT signaling pathway. PMID: 29901196
  33. These results suggest that miR214 mediates vascular inflammation and apoptosis via PTEN expression. PMID: 29916551
  34. A novel information on the susceptibility of PTEN to the inflammatory oxidant HOCl and its effects on the structure and activity of the protein is provided. PMID: 29298524
  35. Study proposes a new mechanism by which loss of PTEN and consequent activation of the PI3K-AKT-mTORC1-S6K1 signalling pathway impairs DNA repair by downregulation of MRE11. PMID: 28967905
  36. In prostate tumor tissue microarrays, loss of PTEN phosphohydrolase (PTEN) correlates with increased tyrosine kinase 6 PTK6 tyrosine 342 (PY342) phosphorylation and poor outcome. PMID: 29142193
  37. in silico analysis revealed PTEN to be the downstream target of miR-21, which was further confirmed by expression analysis. PMID: 29807978
  38. The decreased PTEN was associated with poorer survival outcomes of patients with kidney cancer and PTEN acts as a tumor suppressor in tumorigeneses and progression in kidney cancer. PMID: 29408173
  39. MiR-221 together with proteins MDR1 and ABCG2 was upregulated in Cisplatin-resistant A549 lung cancer cells. Anti-miR-221 inhibits proliferation and induces senescence in lung cancer cells. PTEN/Akt pathway axis was identified as a target of drug resistance induced by miR-221. PMID: 29876362
  40. These results demonstrate that SPAG6 silencing induces PTEN expression to regulate apoptosis though the PI3K/AKT pathway, indicating that SPAG6 may be a potential therapeutic target for myelodysplastic syndromes. PMID: 29749435
  41. The inhibition of PTEN also reduced the cancer effects of CD4+ T cells on non-small cell lung cancer (NSCLC) cell lines following miR-142-5p downregulation. Therefore, our study demonstrated that miR-142-5p regulated CD4+ T cells in human NSCLC through PD-L1 expression via the PTEN pathway. PMID: 29767245
  42. a statistically significant association between PTEN loss and the triple negative breast cancers was found in African American women PMID: 29653745
  43. miR-130b was upregulated in the lupus nephritis group, compared with that in the control group. PTEN was identified as a virtual target of miR-130b, and there was a negative regulatory association between miR-130b and PTEN. miR-130b and PTEN interfered with the viability and apoptosis of mesangial cells. PMID: 29620214
  44. The results of the present study indicate that the expression of miRNA23a may regulate acute myocardial infarction (AMI) through targeting PTEN in patients and in vitro, and PTEN/miRNA23a may therefore be potential targets for the clinical treatment of AMI. PMID: 29488607
  45. TRPC1 regulated HIF1alpha levels in PTEN-deficient MDA-MB-468 and HCC1569 breast cancer cell lines. This regulation arises from effects on the constitutive translation of HIF1alpha under normoxic conditions via an Akt-dependent pathway. PMID: 28559303
  46. miR367 was revealed to bind directly to phosphatase and tensin homolog (PTEN) mRNA and regulate the expression of the PTEN protein PMID: 29512776
  47. The present study confirmed that pAURKA is important in the development of gastric adenocarcinoma and revealed a novel functional link between PTEN, AURKA and pAURKA activation PMID: 29512701
  48. study found that CKS2 knockdown induced PTEN up-regulation and may associate with P53 pathway activation PMID: 29487004
  49. Study showed for the first time that the suppression of rheumatiod arthritis fibroblast-like synoviocyte was mediated by phosphatase and tensin homolog involving survivin silencing. PMID: 28337018
  50. The overexpression of PTEN concomitant with Livin gene silencing was confirmed as a feasible and effective in vitro and in vivo gene modulation method, which may represent a potential therapeutic strategy for the treatment of Gastric Cancer. PMID: 29436592
  51. miR-21-5p mediates apoptosis by targeting PTEN and TIMP3. PMID: 29393355
  52. miR-181 may be a novel and important regulator of cisplatin-resistant non-small cell lung cancer by serving a role in the regulation of apoptosis, as an established rate-limiting miRNA target. PMID: 29484437
  53. PTEN expression is inversely correlated with miR-28 expression levels in gastric cancer tissues. PMID: 29257342
  54. PTEN mutation is associated with Riley Ruvalcaba syndrome and diffuse testicular microlithiasis and precocious puberty. PMID: 29194042
  55. the expression of miR-500a was related to the proliferation and metastasis of hepatocarcinoma, which may be partly because of the activation of AKT/mTOR pathway through targetting PTEN PMID: 29175997
  56. miR-21 may promote salivary adenoid cystic carcinoma progression via PDCD4 and PTEN down-regulation and Bcl-2 up-regulation. PMID: 29328455
  57. These results demonstrated that shikonin inhibits CML proliferation and migration and induces apoptosis by the PTEN/PI3K/AKT pathway, revealing the effects of shikonin therapy on CML. PMID: 29843123
  58. We conclude that miR-486-5p stimulates cell proliferation, migration, and invasion through inhibition of PTEN expression and activation of the oncogenic PI3K/Akt pathway in cervical cancer. Our findings implicate serum miR-486-5p as a novel molecular biomarker that may provide effective approaches to both diagnosis and treatment in cervical cancer PMID: 29316891
  59. The results of this work suggest that PTEN allelic loss is an important mechanism in the late stage of the development of oral potentially malignant lesions into oral cancer PMID: 28858374
  60. This study identifies novel mechanistic biomarkers of PI3Kb inhibition in PTEN-null tumors supporting the concept that targeting PI3Kb may exploit a metabolic dependency that contributes to therapeutic benefit in inducing cell stress PMID: 28972046
  61. miR222 may act as an oncomir in NPC by targeting PTEN in nasopharyngeal carcinoma. PMID: 29115464
  62. Results showed that PTEN expression was positively associated with the expression of RBM38 in breast cancer tissues and breast cancer cells. Moreover, RBM38 stabilized PTEN transcript to enhance its expression via binding to multiple AU/U- rich elements in 3'-untranslated region of PTEN transcript. PMID: 29052531
  63. High PTEN expression is associated with non-small cell lung cancer metastasis. PMID: 28534966
  64. Knockdown of MTDH expression also upregulated PTEN and suppressed pAKT protein expression in Caki2 cells. In conclusion, the present study is the first, to the best of our knowledge, to provide evidence suggesting that miRNA30a5p suppresses tumor human renal cancer cell proliferation via the MTDH/PTEN/AKT pathway PMID: 29207012
  65. These results suggest that the miR-130b inhibitor suppressed glioma cell proliferation and invasion via the PTEN/AKT pathway. Therefore, miR130b is suggested to be an effective therapeutic target for glioma. PMID: 29115407
  66. findings showed that PTEN inhibits HBV replication as well as HBV HCV co-replication. SREBP-1 is involved in HBV HCV replication inhibition by PTEN PMID: 29803738
  67. mechanism study shows that STX3 binds to PTEN and increases PTEN ubiquitination and degradation, thus leading to activation of the PI3K-Akt-mTOR signaling. Therefore, STX3 promotes the growth of breast cancer cells by regulating the PTEN-PI3K-Akt-mTOR signaling. PMID: 29408595
  68. PTEN-long tumor suppressor can repress cancer glial cells proliferation. PMID: 28631420
  69. USP10 directly interacted with and stabilized PTEN via deubiquitination. PMID: 28852924
  70. We identified a novel PTEN missense mutation, c.1A>G (p.M1V), in a 16-year-old girl. The patient with this mutation presented with Autism Spectrum Disorder, intellectual disability, language delay, extreme macrocephaly. PMID: 28774669
  71. Data suggest that low levels of PTEN and KLLN are associated with both sporadic papillary thyroid carcinoma and multi-nodular goiter (as compared to control subjects); however, PTEN and KLLN should not be considered as circulating biomarkers for differential diagnosis between malignancy and benignity in indeterminate thyroid nodules. (PTEN = phosphatase and tensin homolog; KLLN = DNA replication inhibitor killin) PMID: 28755140
  72. our results indicated that miR-1297 functioned as an oncogene in regulating the proliferation, cell cycle and apoptosis of breast cancer (BC) via targeting PTEN/PI3K/AKT signaling, and may represent a novel potential therapeutic target and prognostic marker for BC PMID: 28791363
  73. Data revealed that PTEN was subject to posttranscriptional regulation by miR106b in malignant melanoma (MM) cells. PMID: 29192321
  74. Our results suggested that BetA was able to enhance radiosensitization at least partially by downregulating Sp1 and upregulating PTEN through inducing Sp1 sumoylation. BetA is suggested to be a promising drug for increasing radiosensitization in oral squamous cell carcinoma radiotherapy. PMID: 28791404
  75. We applied the Dual-luciferase Reporter assay to expose the direct interaction between miR-93 and PTEN, which suggested that miR-93 contributes to inducing EMT and drug resistance of breast cancer cells by targeting PTEN PMID: 28765915
  76. Upregulation of plasma miR-93-5p expression significantly increases the risk of esophageal cancer and is associated with poor prognosis. miR-93-5p transferred by exosomes promotes the proliferation of recipient esophageal cancer cells and affects the expression of PTEN and its downstream proteins p21 and cyclin D1. PMID: 29673440
  77. Decreased PTEN expression in the colonic mucosa might contribute to the pathogenesis of microscopic colitis. PMID: 29204743
  78. PTEN as a mechanistically diverse regulator of endocytic trafficking, combining versatile phosphatase- dependent functions with a phosphatase-independent role that ensures homeostasis of signaling receptors in the cell. PMID: 29117568
  79. Study identified PTEN as a direct target of miR-19a in ovarian cancer (OC) cells. PTEN expression is inversely correlated with the expression of miR-19a in OC tissues; its overexpression attenuated the promotion effect of miR-19a on the growth of OC cells. PMID: 29783075
  80. decreased PTEN expression was associated with higher number of recurrence episodes in non-invasive low-grade papillary urothelial carcinoma of the bladder PMID: 29368083
  81. We conducted a 4-year multicenter prospective study of incident patients with features of Cowden/Cowden-like (CS/CS-like) and Bannayan-Riley-Ruvalcaba syndromes (BRRS) without PTEN mutations. Pathogenic or likely pathogenic cancer susceptibility gene alterations were found in 7 of the 87 (8%) CS/CS-like and BRRS patients and included MUTYH, RET, TSC2, BRCA1, BRCA2, ERCC2 and HRAS. PMID: 29684080
  82. Which was correlated with the inhibition of PETN/PI3K/Akt level. PMID: 27698265
  83. MiRNA-20a may have great potential as therapeutic target for coronary artery disease, since its participation can induce alteration of functional genes as well as PTEN. PMID: 28888922
  84. PTEN inhibits replicative senescence-induced MMP-1 expression by regulating NOX4-mediated reactive oxygen species in human dermal fibroblasts. PMID: 28557373
  85. Low Expressions of PTEN is associated with metastasis of esophageal squamous cell carcinoma. PMID: 28418888
  86. miR-198 may promote proliferation and contribute to systemic lupus erythematosus progression by targeting PTEN. PMID: 28944868
  87. results showed that upregulated lncARSR promotes doxorubicin resistance in HCC via modulating PTEN-PI3K/Akt pathway, and implied that lncARSR may serve as a promising prognostic biomarker and therapeutic target for HCC chemo-resistance. PMID: 28464252
  88. overexpression of ciRS-7 blocked the miR-7-induced tumor suppression in MGC-803 and HGC-27 cells and led to a more aggressive oncogenic phenotype, via antagonizing miR-7-mediated PTEN/PI3K/AKT pathway. PMID: 28608528
  89. The reducing system of PTEN was comprised of NADPH, thioredoxin reductase (TrxR1), and thioredoxin (Trx). PMID: 28774816
  90. Exosomes derived from cisplatin-resistant OSCC cells transferred miR-21 to oral squamous cell carcinoma (OSCC) parental cells and induced cisplatin resistance by targeting phosphatase and tensin homolog (PTEN) and programmed cell death 4 (PDCD4). PMID: 28910982
  91. The detection of PTEN hypermethylation could be an early tumorigenesis marker for breast cancer patients. PMID: 27620353
  92. PTEN promotes DNA repair through Rad51-dependnent homologous recombination. PMID: 28759753
  93. The expression levels of Bcl11a and Mdm2, Pten in B-ALL patients with CR were decreased significantly when compared with the healthy control (P < 0.05). PMID: 28544358
  94. The authors conclude that the PTEN-Akt1-mTOR pathway does not appear to play a central role in mitochondrial efficiency in ocular hypertension. PMID: 28499984
  95. Cells with mutated PTEN showed over-activation of the PI3K/mTOR pathway. These cells were more sensitive to PARP inhibition compared to PTEN wild-type cells. In addition, PI3K inhibitor treatment reduced RAD51 foci formation in PTEN mutated cells, and sensitized these cells to PARP inhibitor. PMID: 28886696
  96. Our results indicated that miR-144 can regulate the proliferation and apoptosis of trophoblastic cells through targeting the PTEN signaling pathway. PMID: 28772212
  97. The results show that PTEN controls multicellular assembly through a membrane-associated regulatory protein complex composed of beta-Arrestin1, ARHGAP21 and Cdc42. PMID: 28749339
  98. Our observations of these PTEN intronic variants should contribute to the determination of pathogenicity of PTEN intronic variants and aid in genetic counseling PMID: 28677221
  99. PIK3CA and/or ERBB family mutations were detected in 23 (31.1%) tumour samples tested, whereas PTEN expression was low in 31.1% of cases tested PMID: 28750640
  100. Hypoxia-induced upregulation of miR-214 was found to promote pulmonary hypertension development by targeting PTEN in pulmonary artery smooth muscle cells. PMID: 28684904

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Involvement in disease Cowden syndrome 1 (CWS1); Lhermitte-Duclos disease (LDD); Bannayan-Riley-Ruvalcaba syndrome (BRRS); Squamous cell carcinoma of the head and neck (HNSCC); Endometrial cancer (ENDMC); Glioma 2 (GLM2); VACTERL association with hydrocephalus (VACTERL-H); Prostate cancer (PC); Macrocephaly/autism syndrome (MCEPHAS)
Subcellular Location Cytoplasm, Nucleus, Nucleus, PML body, Note=Monoubiquitinated form is nuclear, Nonubiquitinated form is cytoplasmic, Colocalized with PML and USP7 in PML nuclear bodies (PubMed:18716620), XIAP/BIRC4 promotes its nuclear localization (PubMed:19473982), SUBCELLULAR LOCATION: Isoform alpha: Secreted
Tissue Specificity Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
Database Links

HGNC: 9588

OMIM: 137800

KEGG: hsa:5728

STRING: 9606.ENSP00000361021

UniGene: Hs.500466

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