PTEN (Ab-370) Antibody

Datasheet
Code CSB-PA000679
Product Type Polyclonal Antibody
Size US$297
Uniprot No. P60484
Image
  • Western blot analysis of extracts from C2C12 cells using PTEN(Ab-370) Antibody.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PTEN(Ab-370) Antibody(left) or the same antibody preincubated with blocking peptide(right).
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunogen Peptide sequence around aa.368~372 (D-V-S-D-N) derived from Human PTEN.
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Specificity The antibody detects endogenous level of total PTEN protein.
Tested Applications ELISA,WB,IHC;WB:1:500-1:1000,IHC:1:50-1:200
Relevance Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue.

Al-Khouri AM, et al. (2005). J Biol Chem. 280 (42):35195-35202.
Miller SJ, et al. (2002). FEBS Lett. 528 (1-3): 145-153.
Torres J, et al. (2001). J Biol Chem.276 (2): 993-998.
Vazquez F, et al. (2000). Mol Cell Biol.20 (14): 5010-5018.
Form Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality Polyclonal
Alias MMAC1; Mutated in multiple advanced cancers 1; Protein-tyrosine phosphatase PTEN; TEP1;
Immunogen Species Homo sapiens (Human)
Target Names PTEN
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4
Involvement in disease Cowden syndrome 1 (CWS1); Lhermitte-Duclos disease (LDD); Bannayan-Riley-Ruvalcaba syndrome (BRRS); Squamous cell carcinoma of the head and neck (HNSCC); Endometrial cancer (ENDMC); Glioma 2 (GLM2); VACTERL association with hydrocephalus (VACTERL-H); Prostate cancer (PC); Macrocephaly/autism syndrome (MCEPHAS)
Subcellular Location Cytoplasm, Nucleus, Nucleus, PML body, Note=Monoubiquitinated form is nuclear, Nonubiquitinated form is cytoplasmic, Colocalized with PML and USP7 in PML nuclear bodies (PubMed:18716620), XIAP/BIRC4 promotes its nuclear localization (PubMed:19473982), SUBCELLULAR LOCATION: Isoform alpha: Secreted
Tissue Specificity Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
Database Links

HGNC: 9588

OMIM: 137800

KEGG: hsa:5728

STRING: 9606.ENSP00000361021

UniGene: Hs.500466

Pathway mTOR signaling pathway
p53 signaling pathway
PI3K-Akt signaling pathway
Focal adhesion
Nitric oxide signaling
Autophagy
Cellular senescence
FoxO signaling pathway
Phosphatidylinositol signaling system
Sphingolipid signaling pathway

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