Phospho-ABL1 (Tyr204) Antibody

Datasheet
Code CSB-PA984556
Product Type Polyclonal Antibody
Size US$360
Uniprot No. P00519
Image
  • Western blot analysis of extracts from COS7 cells treated with Adriamycin using Abl (Phospho-Tyr204) antibody.The lane on the right is treated with the antigen-specific peptide.
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunogen Peptide sequence around phosphorylation site of tyrosine 204 (K-L-Y(p)-V-S) derived from Human c-Abl.
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Specificity The antibody detects endogenous level of Abl only when phosphorylated at tyrosine 204.
Tested Applications ELISA,WB;WB:1:500-1:1000
Relevance

Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 (involved in branch formation); ANXA1 (involved in membrane anchoring); DBN1, DBNL, CTTN, RAPH1 and ENAH (involved in signaling); or MAPT and PXN (microtubule-binding proteins).

Meyn MA, et al. (2006) J Biol Chem 281, 30907-16
Steen H, et al. (2003) Mol Cell Proteomics 2, 138-45
Salomon AR, et al. (2003) Proc Natl Acad Sci U S A 100, 443-8

Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Clonality Polyclonal
Alias p150; c-ABL; EC 2.7.10.2;
Immunogen Species Homo sapiens (Human)
Target Names ABL1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Still Have Questions? Leave a Message or Start an on-line Chat
Function Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 (involved in branch formation); ANXA1 (involved in membrane anchoring); DBN1, DBNL, CTTN, RAPH1 and ENAH (involved in signaling); or MAPT and PXN (microtubule-binding proteins). Phosphorylation of WASF3 is critical for the stimulation of lamellipodia formation and cell migration. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as BCAR1, CRK, CRKL, DOK1, EFS or NEDD9. Phosphorylates multiple receptor tyrosine kinases and more particularly promotes endocytosis of EGFR, facilitates the formation of neuromuscular synapses through MUSK, inhibits PDGFRB-mediated chemotaxis and modulates the endocytosis of activated B-cell receptor complexes. Other substrates which are involved in endocytosis regulation are the caveolin (CAV1) and RIN1. Moreover, ABL1 regulates the CBL family of ubiquitin ligases that drive receptor down-regulation and actin remodeling. Phosphorylation of CBL leads to increased EGFR stability. Involved in late-stage autophagy by regulating positively the trafficking and function of lysosomal components. ABL1 targets to mitochondria in response to oxidative stress and thereby mediates mitochondrial dysfunction and cell death. In response to oxidative stress, phosphorylates serine/threonine kinase PRKD2 at 'Tyr-717'
Involvement in disease Leukemia, chronic myeloid (CML); Congenital heart defects and skeletal malformations syndrome (CHDSKM)
Subcellular Location Cytoplasm, cytoskeleton, Nucleus, Mitochondrion, Note=Shuttles between the nucleus and cytoplasm depending on environmental signals, Sequestered into the cytoplasm through interaction with 14-3-3 proteins, Localizes to mitochondria in response to oxidative stress (By similarity), SUBCELLULAR LOCATION: Isoform IB: Nucleus membrane, Lipid-anchor
Protein Families Protein kinase superfamily, Tyr protein kinase family, ABL subfamily
Tissue Specificity Widely expressed.
Database Links

HGNC: 76

OMIM: 189980

KEGG: hsa:25

STRING: 9606.ENSP00000361423

UniGene: Hs.431048

Pathway ErbB signaling pathway
Cell cycle
Ras signaling pathway
Neurotrophin signaling pathway

Related Products

ABL1 Antibodies

ABL1 Antibodies for Human

ABL1 Antibodies for

ABL1 Antibodies for Homo sapiens (Human)

ABL1 Proteins

ABL1 Proteins for Homo sapiens (Human)

ABL1 ELISA Kit

ABL1 ELISA Kit for Homo sapiens (Human)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.