ABL1, a non-receptor tyrosine kinase encoded by the ABL1 gene, is widely expressed in various cells, particularly in hematopoietic cells. It regulates cellular processes such as signaling, cycle control, differentiation, and apoptosis by phosphorylating substrate proteins. ABL1's abnormal activation is linked to cancer development, especially Philadelphia chromosome-positive chronic myeloid leukemia, which results from the fusion of the ABL1 and BCR genes, creating a constitutively active kinase. This fusion protein drives uncontrolled cell proliferation, and ABL1 is the target of therapies like Imatinib, used to treat CML and other ABL1-related cancers by inhibiting its kinase activity.
Recombinant Human Tyrosine-protein kinase ABL1 (ABL1), partial (CSB-RP043554h)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
ABL1 Antibody (CSB-PA04355A0Rb)
Validated Data
IHC image of CSB-PA04355A0Rb diluted at 1:1200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating ABL1 in Hela whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA04355A0Rb in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA04355A0Rb (6µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)
Human Tyrosine-protein kinase ABL1(ABL1) ELISA kit (CSB-EL001105HU)
Validated Data
Code: CSB-EL001105HU
Size: 96T,5×96T,10×96T
Sensitivity: 6.25 pg/mL
Detection Range: 25 pg/mL-1600 pg/mL
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
The following ABL1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
ABL1 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA04355A0Rb | ABL1 Antibody | Human | ELISA, IHC, IP |
CSB-PA04355D0Rb | ABL1 Antibody, Biotin conjugated | Human | ELISA |
CSB-PA980598 | Phospho-ABL1 (Tyr412) Antibody | Human, Mouse, Rat | ELISA, IHC, IF |
CSB-PA984556 | Phospho-ABL1 (Tyr204) Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA573869 | Phospho-ABL1 (Tyr245) Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA001140 | ABL1 Antibody | Human,Mouse,Rat,Monkey | WB, IHC, ELISA |
CSB-PA001141 | ABL1 Antibody | Human,Mouse,Rat | WB, IHC, IF, ELISA |
CSB-PA008490 | Phospho-ABL1 (Y204) Antibody | Human,Mouse,Rat,Monkey | WB, IHC, ELISA |
CSB-PA008492 | Phospho-ABL1 (T735) Antibody | Human,Mouse,Rat,Monkey | WB, IHC, ELISA |
CSB-PA008493 | Phospho-ABL1 (Y245) Antibody | Human,Mouse,Rat | WB, IF, ELISA |
CSB-PA008496 | ABL1 Antibody | Human,Mouse,Rat | IHC, ELISA |
CSB-PA008499 | ABL1 Antibody | Human,Mouse,Rat,Monkey | WB, ELISA |
CSB-PA547318 | ABL1 Antibody | Human,Mouse | ELISA,IHC |
CSB-PA959474 | ABL1 Antibody | Human,Mouse,Rat | IHC, ELISA |
ABL1 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP001105HU CSB-EP001105HU CSB-BP001105HU CSB-MP001105HU CSB-EP001105HU-B |
Recombinant Human Tyrosine-protein kinase ABL1 (ABL1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-RP043554h | Recombinant Human Tyrosine-protein kinase ABL1 (ABL1), partial | E.coli |
ABL1 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP001105MO CSB-EP001105MO CSB-BP001105MO CSB-MP001105MO CSB-EP001105MO-B |
Recombinant Mouse Tyrosine-protein kinase ABL1 (Abl1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ABL1 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-EL001105HU | Human Tyrosine-protein kinase ABL1(ABL1) ELISA kit | serum, plasma, tissue homogenates | 6.25 pg/mL |