Phospho-FOXO1/FOXO3/FOXO4 (Thr24/32) Antibody

Datasheet
Code CSB-PA196080
Product Type Polyclonal Antibody
Size US$360
Uniprot No. Q12778
Image
  • Western blot analysis of extracts from 293 cells treated with Serum using FOXO1/3/4-pan (Phospho-Thr24/32) Antibody.The lane on the right is treated with the antigen-specific peptide.
Immunogen Peptide sequence around phosphorylation site of threonine 24/32(S-C-T(p)-W-P) derived from Human FOXO1/3/4-pan .
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Specificity The antibody detects endogenous levels of FOXO1/3/4-pan only when phosphorylated at threonine 24/32.
Tested Applications ELISA,WB;WB:1:500-1:1000
Relevance

Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts syngernistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A to activate the expression of genes such as IGFBP1, G6PC and PPCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1. Promotes neural cell death. Mediates insulin action on adipose. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells.

Robert J. Southgate, J. Biol. Chem., Jul 2007; 282: 21176 - 21186.
Susumu Kodama, Mol. Cell. Biol., Sep 2004; 24: 7931 - 7940.
Dragan Marinkovic, J. Clin. Invest., Aug 2007; 117: 2133 - 2144.
Marie-Liesse Asselin-Labat, Blood, Jul 2004; 104: 215 - 223.

Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Clonality Polyclonal
Alias FKHR; FOXO1; FKHR2; AFX; MLLT7
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Target Names FOXO1/FOXO3/FOXO4
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC and PCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1. Promotes neural cell death. Mediates insulin action on adipose tissue. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells. Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner. Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling (By similarity).
Involvement in disease Rhabdomyosarcoma 2 (RMS2)
Subcellular Location Cytoplasm, Nucleus
Tissue Specificity Ubiquitous.
Database Links

HGNC: 3819

OMIM: 136533

KEGG: hsa:2308

STRING: 9606.ENSP00000368880

UniGene: Hs.370666

Pathway Cellular senescence
AGE-RAGE signaling pathway in diabetic complications
AMPK signaling
FoxO signaling pathway
Glucagon signaling pathway
Insulin signaling pathway

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