Phospho-GRIN2B (Tyr1474) Antibody

Code CSB-PA969425
Size US$360
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  • Immunofluorescence staining of methanol-fixed Hela cells using NMDAR2B(phospho-Tyr1474) antibody.
  • Western blot analysis of extracts from A431 cells untreated or treated with EGF using NMDAR2B (phospho-Tyr1474) Antibody.
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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) GRIN2B Polyclonal antibody
Uniprot No.
Target Names
GRIN2B
Alternative Names
AW490526 antibody; EIEE27 antibody; Glutamate [NMDA] receptor subunit epsilon 2 antibody; Glutamate [NMDA] receptor subunit epsilon-2 antibody; Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody; Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody; Glutamate receptor ionotropic NMDA2B antibody; Glutamate receptor subunit epsilon 2 antibody; Glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody; GRIN 2B antibody; GRIN2B antibody; hNR 3 antibody; hNR3 antibody; MGC142178 antibody; MGC142180 antibody; MRD6 antibody; N methyl D asparate receptor channel subunit epsilon 2 antibody; N methyl D aspartate receptor subtype 2B antibody; N methyl D aspartate receptor subunit 2B antibody; N methyl D aspartate receptor subunit 3 antibody; N-methyl D-aspartate receptor subtype 2B antibody; N-methyl-D-aspartate receptor subunit 3 antibody; NMDA NR2B antibody; NMDA R2B antibody; NMDAR2B antibody; NMDE2 antibody; NMDE2_HUMAN antibody; NME2 antibody; NR2B antibody; NR3 antibody
Raised in
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Peptide sequence around phosphorylation site of Tyr1474 (H-V-Y(p)-E-K) derived from Human NMDAR2B.
Immunogen Species
Homo sapiens (Human)
Clonality
Polyclonal
Purification Method
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Concentration
It differs from different batches. Please contact us to confirm it.
Form
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Tested Applications
ELISA,WB,IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:1000
IF 1:100-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death. Contributes to neural pattern formation in the developing brain. Plays a role in long-term depression (LTD) of hippocampus membrane currents and in synaptic plasticity.
Gene References into Functions
  1. This study demonstrated significant differences in the blood levels of GRIN2B between people with epilepsy and healthy subjects. PMID: 30140987
  2. There was no significant association between selected GRIN2B polymorphisms and personality traits. PMID: 29921740
  3. No association between GRIN2B rs1805502 polymorphism and OCD, symptom dimensions or treatment response in South Indian patients. PMID: 29321103
  4. Results showed that NRG1-ErbB4 signaling suppressed phosphorylation of GluN2B at position 1472 by Src kinase, and decreased levels of phosphorylation level of GluN2B and Src were detected in human symptomatic epilepsy tissues. PMID: 28273943
  5. Results suggest that alterations in the glutamate and dopamine system (GRIN2B and DRD4) in attention-deficit/hyperactivity disorder may contribute to abnormalities in local functional connectivity and its dynamic repertoire in the superior parietal area, and these abnormalities would be related to dysfunction in sustained and divided attention. PMID: 28258362
  6. GRIN2B SNP rs219927 is associated with brain volume in the left posterior cingulate cortex in adolescent alcohol dependence. PMID: 27498914
  7. The results suggest that the GRIN2B gene may modify the linguistic processes involved in the retrieval of information from the mental lexicon on the basis of semantic traits and, moreover, contribute to the variability of clinical symptoms of impairment of abstract thinking in patients with schizophrenia. PMID: 27240047
  8. It is this DAPK1-NR2B interaction that arbitrates the pathological processes like apoptosis, necrosis, and autophagy of neuronal cells observed in stroke injury, hence we aimed to inhibit this vital interaction to prevent neuronal damage. PMID: 28858643
  9. Five rare missense mutations in the GRIN2B gene are not associated with schizophrenia or autism spectrum disorder in a Japanese population. PMID: 27616045
  10. NR4A1 knockdown partly decreased surface NR2B by promoting NR2B internalization. PMID: 27876882
  11. This review showed that GRIN2B associate with Obsessive-compulsive disorder. PMID: 28608743
  12. GRIN2B polymorphism was associated with a twofold increase in odds of a Suicide Attempts in Alcohol-dependent individuals. PMID: 28558131
  13. GRIN2B encephalopathy is also frequently associated with movement disorder, cortical visual impairment and malformation of cortical development revealing novel phenotypic consequences of channelopathies. PMID: 28377535
  14. mutations of the human NMDAR were investigated and studied their consequences with kinetic modelling and electrophysiology. PMID: 29511171
  15. The polymorphisms in HTR2A and GRIN2B were significantly associated with increased risk of chronic obstructive pulmonary disease. PMID: 28900078
  16. In vitro DNA-binding and reporter gene assays of different allele combinations in transfected cells showed that the A allele was a gain-of-function variant associated with increasing GRIN2B mRNA levels PMID: 28439047
  17. Polymorphism C366G of gene GRIN2B and verbal episodic memory: No association with schizophrenia PMID: 29368832
  18. These preliminary results demonstrated that the GRIN2B gene may confer to some extent the susceptibility to OCD and its symptoms. PMID: 27394963
  19. Our finding confirms that early-onset EE may be caused not only by gain-of-function variants but also by splice site mutations-in particular those affecting the splice acceptor site of the 10th intron of the GRIN2B gene. PMID: 27605359
  20. Results indicate that the GRIN2B single nucleotide polymorphisms rs890 might be associated with schizophrenia in the Chinese Han population. PMID: 27453061
  21. These in vivo changes reflect changes in glutamate transporter protein in Huntington's disease (HD), mice and human HD post-mortem tissue. Furthermore, NAC was able to rescue changes in key glutamate receptor proteins related to excitotoxicity in HD, including NMDAR2B. PMID: 27179791
  22. Genetic association of CYP2B6*6 and GRIN2B (rs1019385 and rs1806191) single-nucleotide polymorphisms and ketamine-induced EP occurrence. PMID: 28252572
  23. In this review, mutations in GRIN2B were described as distributed throughout the entire gene in patients with neuropsychiatric and developmental disorders. PMID: 27818011
  24. Genetic variants were found in GluN2B from patients with neurological or psychiatric disorders resulting in reduced surface expression of GluN2B. PMID: 28283559
  25. SNP rs1806201 associated with earlier age at onset of withdrawal symptoms in Indian alcohol dependent subjects PMID: 26771436
  26. Multiple genetic variants in GRIN2B are jointly associated with gene expression, prefrontal function and behaviour during working memory (WM). These results support the role of GRIN2B genetic variants in WM prefrontal activity in human adults. PMID: 26690829
  27. Findings do not support a role for GRIN2B variants in altering response to clozapine in our sample of European patients with schizophrenia deemed resistant or intolerant to treatment PMID: 26876050
  28. both Arf6 activation through GluN2B-BRAG1 during early development and the transition from BRAG1- to BRAG2-dependent Arf6 signaling induced by the GluN2 subunit switch are critical for the development of mature glutamatergic synapses. PMID: 26884337
  29. GRIN2B (rs7301328) was significantly associated with disruptive behavior in adolescents. PMID: 26819771
  30. GRIN2B polymorphisms do not influence Parkinson disease age of onset in the US Caucasian population. PMID: 26627941
  31. GluN2B appears to exhibit allosteric regulation/inhibition by two distinct classes of antagonists; data from in silico docking suggest that GluN2B-selective antagonists broadly divide into two distinct classes according to binding pose. PMID: 26912815
  32. GRIN2B encodes the NR2 subunit of NMDA receptors, which are a class of ionotropic glutamate receptors involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission. PMID: 26321256
  33. ligand binding to cell surface GD2 induces rapid and transient activation of Src-family kinases, followed by Src-dependent phosphorylation of NMDA-receptor NR2B subunits selectively. PMID: 26252487
  34. Study provides evidence of an association between GRIN2B polymorphisms and Schizophrenia susceptibility and symptoms in the Han Chinese population. PMID: 26020650
  35. Polymorphisms in DRD1, DRD2 and GRIN2B confer increased risk of impulse control problems among PD patients. PMID: 25896831
  36. BDNF, APOE, and HSP70-1 genes, but not GRIN2B, might be associated with a risk of POAG occurrence in the Polish population PMID: 25893192
  37. This study provides further evidence in support of GRIN2B contributing toward Developmental Dyslexia and deficits in Developmental Dyslexia PMID: 25426763
  38. common variants and related haplotypes of GRIN2B are associated with autism risk. PMID: 25656819
  39. did not find significant pooled Odds Ratios for any of the six genes, under different models and stratifying for ethnicity. PMID: 25660313
  40. 421C/A polymorphism not associated with risk of occurrence of primary open-angle glaucoma PMID: 25693086
  41. The GluN2B-containing NMDA receptors (GluN2B/GluN1) were increased in schizophrenia in hippocampal CA3 tissue, but not in hippocampal CA1 tissue. PMID: 25585032
  42. The major depression subjects exhibited significantly higher expression levels of the NMDA receptor subunit genes GRIN2B. PMID: 24925192
  43. The results demonstrate the contribution of Grin2b via DISC1 on the risk of Schizophrenia PMID: 23855403
  44. first evidence of GluN2 protein abnormalities in the hippocampus in schizophrenia, highlighting the hippocampal lateralisation in this disorder. Only left hippocampus deficient in GluN2B. PMID: 25292222
  45. observations provide an initial delineation of the behavioral phenotype of GRIN2B mutation carriers. PMID: 23718928
  46. BDNF/TrkB signaling plays an important role in the NRG1-stimulated NR2B regulation. PMID: 25052836
  47. Association of GRIN2B gene polymorphisms with paranoid schizophrenia and response to common neuroleptics in Russians and Tatars from Bashkortostan Republic. PMID: 25486778
  48. Genetic polymorphisms and isogenic deletions of loop-bound sequences conferred liability for cognitive performance and decreased GRIN2B expression. PMID: 25467983
  49. Results advocate that genetic variability in GRIN2B gene, involved in synaptic functioning, might provide valuable insights into disease pathogenesis, continuing to attract significant attention in biomedical research on its genetic and functional role PMID: 24292895
  50. GRIN2B is likely to be related to neuro-developmental anomalies. PMID: 24503147

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Involvement in disease
Mental retardation, autosomal dominant 6, with or without seizures (MRD6); Epileptic encephalopathy, early infantile, 27 (EIEE27)
Subcellular Location
Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Late endosome. Lysosome. Cytoplasm, cytoskeleton.
Protein Families
Glutamate-gated ion channel (TC 1.A.10.1) family, NR2B/GRIN2B subfamily
Tissue Specificity
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
Database Links

HGNC: 4586

OMIM: 138252

KEGG: hsa:2904

STRING: 9606.ENSP00000279593

UniGene: Hs.504844

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