Phospho-ITCH (Tyr420) Antibody

Datasheet
Code CSB-PA180347
Product Type Polyclonal Antibody
Size US$360
Uniprot No. Q96J02
Image
  • Immunohistochemical analysis of paraffin-embedded human brain tissue using ITCH (Phospho-Tyr420) antibody (left)or the same antibody preincubated with blocking peptide (right).
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunogen Peptide sequence around phosphorylation site of tyrosine 420(F-I-Y(p)-G-N) derived from Human ITCH .
Raised in Rabbit
Species Reactivity Human,Mouse
Specificity The antibody detects endogenous levels of ITCH only when phosphorylated at tyrosine 420.
Tested Applications ELISA,IHC;IHC:1:50-1:100
Relevance

Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain.

Chen X., Genomics 73:238-241(2001).
Deloukas P., Nature 414:865-871(2001).
Wood J.D., Mol. Cell. Neurosci. 11:149-160(1998).

Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Clonality Polyclonal
Alias AIF4; AIP4; NAPP1;
Immunogen Species Homo sapiens (Human)
Target Names ITCH
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
Involvement in disease Autoimmune disease, multisystem, with facial dysmorphism (ADMFD)
Subcellular Location Cell membrane, Peripheral membrane protein, Cytoplasmic side, Cytoplasm, Nucleus, Early endosome membrane, Peripheral membrane protein, Cytoplasmic side, Endosome membrane, Peripheral membrane protein, Cytoplasmic side
Tissue Specificity Widely expressed.
Database Links

HGNC: 13890

OMIM: 606409

KEGG: hsa:83737

STRING: 9606.ENSP00000363998

UniGene: Hs.632272

Pathway TNF signaling pathway
Ubiquitin mediated proteolysis
Endocytosis

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