Code | CSB-RA587302A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
The ITCH recombinant monoclonal antibody is used to detect human ITCH protein in ELISA and WB applications and is produced using recombinant DNA technology. To generate this antibody, the gene encoding the ITCH monoclonal antibody is synthesized by sequencing the cDNA of the ITCH antibody-producing hybridomas. The hybridomas are produced by fusing myeloma cells with B cells isolated from an animal immunized with a synthesized peptide derived from human ITCH. The synthesized gene is then incorporated into a vector and transfected into cells for cultivation. The resulting ITCH recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography.
The ITCH protein plays an important role in regulating protein turnover and degradation by adding ubiquitin molecules to target proteins. ITCH specifically targets a wide range of proteins for degradation, including transcription factors, signaling molecules, and membrane receptors, by recognizing specific motifs or domains in these proteins and catalyzing the attachment of ubiquitin chains. ITCH can also act as a negative regulator of the immune response by promoting the degradation of proteins involved in the activation of T cells and cytokine signaling.
Applications : Immunohistochemistry (IHC)
Sample type: cell
Review: Representative photomicrographs for immunohistochemical staining of ITCH. Invasive ductal carcinoma showing moderate [A] and strong [B] cytoplasmic and membranous staining of CXCR4. Brown color indicates positive staining. Negative, weak, moderate and strong staining = 0, 1, 2 and 3, respectively. Magnification: ×400, scale bar: 20 μm.
By Anonymous