Phospho-TP73 (Tyr99) Antibody

Datasheet
Code CSB-PA251083
Product Type Polyclonal Antibody
Size US$360
Uniprot No. O15350
Image
  • Western blot analysis of extracts from 293 cells untreated or treated with UV using p73(Phospho-Tyr99) Antibody.
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunogen Peptide sequence around phosphorylation site of tyrosine 99 (S-P-Y(p)-A-Q) derived from Human P73.
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Specificity The antibody detects endogenous level of p73 only when phosphorylated at tyrosine99.
Tested Applications ELISA,WB;WB:1:500-1:1000
Relevance

This gene encodes tumor protein p73, which is a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include p53, p63, and p73 and have high sequence similarity to one another, which allows p63 and p73 to transactivate p53-responsive genes causing cell cycle arrest and apoptosis. The family members can interact with each other in many ways involving direct or indirect protein interactions, resulting in regulation of the same target gene promoters or regulation of each other's promoters. The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors. The p73 gene expresses at least 35 mRNA variants due to the use of alternate promoters, alternate translation initiation sites, and multiple splice variations. Theoretically this can account for 29 different p73 isoforms; however, the biological validity and the full-length nature of most variants have not been determined.

Yuan ZM, et al.(1999) Nature.399(6738): 814-817.

Form Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Clonality Polyclonal
Alias TP73; TRP73; p53-related protein; p73-alpha;
Immunogen Species Homo sapiens (Human)
Target Names TP73
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein.
Subcellular Location Nucleus, Cytoplasm
Protein Families P53 family
Tissue Specificity Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue.
Database Links

HGNC: 12003

OMIM: 601990

KEGG: hsa:7161

STRING: 9606.ENSP00000367545

UniGene: Hs.192132

Pathway Hippo signaling pathway
p53 signaling pathway
Neurotrophin signaling pathway

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