RNF5 Antibody

Datasheet

Code	CSB-PA034142
Size	US$166
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Image	The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA034142(RNF5 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA034142(RNF5 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200) Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-5: K562 cells, 231 cells, Jurkat cells, hela cells, 293T cells, Primary antibody: CSB-PA034142(RNF5 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

Q99942

Target Names

RNF5

Alternative Names

RNF5; G16; NG2; RMA1; E3 ubiquitin-protein ligase RNF5; Protein G16; RING finger protein 5; RING-type E3 ubiquitin transferase RNF5; Ram1 homolog; HsRma1

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthetic peptide of Human RNF5

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form

Liquid

Tested Applications

ELISA,WB,IHC

Recommended Dilution

Application	Recommended Dilution
ELISA	1:2000-1:5000
WB	1:500-1:2000
IHC	1:25-1:100

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of STING1 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurs in mitochondria after viral transfection and regulates antiviral responses.

Gene References into Functions

we found that RNF5 is differentially expressed in human bronchial epithelia from CF vs. control patients. Our results identify RNF5 as a target for therapeutic modalities to antagonize mutant CFTR proteins. PMID: 26183966
RNF5 is involved in the regulation of the L-glutamine carrier proteins SLC1A5 and SLC38A2. PMID: 25759021
Our data suggest that RNF5 regulates the turnover of specific G protein-coupled receptors by ubiquitinating JAMP and preventing proteasome recruitment. PMID: 23798571
In addition to confirming one of the top findings in the meta-analysis, TRIM26, RNF5 and HLA-DRB3 have been identified as potential candidate genes for schizophrenia. PMID: 22433715
Rma1, an E3 ubiquitin ligase localized in the ER membrane, is involved in Pendrin degradation PMID: 22750442
JB12 cooperates with cytosolic Hsc70 and the ubiquitin ligase RMA1 to target CFTR and CFTRDeltaF508 for degradation. PMID: 21148293
found in cerebral microvessels endothelium PMID: 11603805
RNF5 regulates cell motility by targeting paxillin ubiquitination and altered localization PMID: 12861019
the G(16)-mediated activation of IKK/NFkappaB by N(6)-cyclohexyladenosine requires a complex signaling network composed of multiple intermediates PMID: 15485865
efficient bacterial escape into the cytosol of epithelial cells requires HsRMA1-mediated SopA ubiquitination and contributes to Salmonella-induced enteropathogenicity PMID: 16176924
RNF5 is a novel regulator of breast cancer progression through its effect on actin cytoskeletal alterations. PMID: 17804730
RNF5 associates with JAMP in the ER membrane. This association results in Ubc13-dependent RNF5-mediated noncanonical ubiquitination of JAMP. PMID: 19269966
E3 ubiquitin ligase RNF5 interacted with MITA in a viral-infection-dependent manner PMID: 19285439

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Subcellular Location

Membrane; Multi-pass membrane protein. Mitochondrion membrane. Endoplasmic reticulum membrane. Note=Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles.

Tissue Specificity

Widely expressed.

Database Links

HGNC: 10068

OMIM: 602677

KEGG: hsa:6048

STRING: 9606.ENSP00000364235

UniGene: Hs.731774

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Proteolysis Targeting Chimera (PROTAC): A Do You Really Know Neurons and Glia Cells in Nerve System? Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Protein processing in endoplasmic reticulum

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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