RYK Antibody

Code CSB-PA020617LA01HU
Size US$166
Order now
Image
  • Western Blot
    Positive WB detected in: MCF-7 whole cell lysate
    All lanes: RYK antibody at 3.3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 68 kDa
    Observed band size: 68 kDa

  • IHC image of CSB-PA020617LA01HU diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA020617LA01HU diluted at 1:200 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

The Latest Promotion Free Antibody trial simple
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) RYK Polyclonal antibody
Uniprot No.
Target Names
RYK
Alternative Names
RYK; JTK5A; Tyrosine-protein kinase RYK
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Tyrosine-protein kinase RYK protein (494-586AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The RYK Antibody (Product code: CSB-PA020617LA01HU) is Non-conjugated. For RYK Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA020617LB01HU RYK Antibody, HRP conjugated ELISA
FITC CSB-PA020617LC01HU RYK Antibody, FITC conjugated
Biotin CSB-PA020617LD01HU RYK Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:200-1:500
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.
Gene References into Functions
  1. our findings demonstrate that Ryk promotes stem cell-like and tumorigenic features to glioma cells its essential for the maintenance of glioblastoma stem cells PMID: 28086236
  2. Wnt5A/Ryk signaling might provide novel therapeutic strategies to prevent capillary leakage in systemic inflammation and septic shock. PMID: 27159116
  3. results corroborated previous findings of Ryk-mediated Wnt5a effect, and suggested a role for Ror2 in the Wnt5a machinery in glioblastoma PMID: 26596412
  4. Early treatment with HGF/IgG complexes after myocardial ischaemia with reperfusion may rescue tissue through vasoprotection conferred by c-Met and RYK signalling PMID: 26025956
  5. Ryk-intracellular domain pathway may impair FOXO protective activity in mutant polyglutamine neurons, suggesting neurons unable to efficiently maintain function and resist disease from the earliest phases of the pathogenic process in Huntington disease PMID: 24960609
  6. is a candidate wnt5a receptor. PMID: 23748645
  7. The Ryk is important for the Wnt-5a-dependent induction of MMP-2 and invasive activity in glioma-derived cells and that Ryk might have a novel patho-physiological function in adult cancer invasion. PMID: 24621529
  8. RYK and VANGL2 proteins form a complex, whereas RYK also activated RhoA, a downstream effector of PCP signaling. PMID: 22773843
  9. RYK interacts both physically and functionally with the E3 ubiquitin MIB1. MIB1 is sufficient to activate Wnt/CTNNB1 signaling and this activity depends on endogenous RYK. PMID: 21875946
  10. RYK, a catalytically inactive receptor tyrosine kinase, associates with EphB2 and EphB3 but does not interact with AF-6. PMID: 11956217
  11. a missense mutation, 1355G>A, and one rare single nucleotide polymorphisms haplotype may play a role in the development of cleft lip and/or palate in the Vietnamese, and cleft lip and/ or palate and cleft palate only in the Japanese PMID: 16681403
  12. validated occurrence of an unusual TG 3' splice site in intron 7 PMID: 17672918

Show More

Hide All

Subcellular Location
Membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm.
Protein Families
Protein kinase superfamily, Tyr protein kinase family
Tissue Specificity
Observed in all the tissues examined.
Database Links

HGNC: 10481

OMIM: 600524

KEGG: hsa:6259

STRING: 9606.ENSP00000296084

UniGene: Hs.654562

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X
Place an order now

I. Product details

*
*
*
*

II. Contact details

*
*

III. Ship To

*
*
*
*
*
*
*

IV. Bill To

*
*
*
*
*
*
*
*