RYK Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA278193A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: A-431 whole cell lysate(30µg), COLO205 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), HeLa whole cell lysate(30µg), HEK293 whole cell lysate(30µg) All lanes: RYK antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 3min Immunofluorescence staining of A-431 cell with CSB-RA278193A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing BxPC-3 cells stained with CSB-RA278193A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

P34925

Target Names

RYK

Alternative Names

D3S3195 antibody; ERK 3 antibody; Hydroxyaryl protein kinase antibody; JTK 5 antibody; JTK 5A antibody; JTK5 antibody; JTK5A antibody; JTK5A protein tyrosine kinase antibody; receptor like tyrosine kinase antibody; receptor like tyrosine kinase precursor antibody; RYK 1 antibody; Ryk antibody; RYK_HUMAN antibody; RYK1 antibody; Tyrosine protein kinase RYK antibody; Tyrosine protein kinase RYK precursor antibody; Tyrosine-protein kinase RYK antibody; Vik antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human RYK

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2H3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

RYK is a receptor tyrosine kinase that plays essential roles in Wnt signaling pathways, influencing cell polarity, migration, and differentiation during development. Unlike conventional receptor tyrosine kinases, RYK functions as a co-receptor for Wnt ligands, making it a compelling target for researchers investigating developmental biology, neural patterning, and cancer progression where Wnt signaling dysregulation is implicated.

This recombinant monoclonal antibody, clone 2H3, offers the reproducibility and consistency that demanding experimental workflows require. Produced using recombinant technology with a defined sequence, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring your results remain comparable across extended studies and collaborative projects.

Validated across multiple applications, this antibody provides flexibility for diverse experimental approaches. Western blot analysis demonstrates clean detection of RYK at the expected 68 kDa molecular weight across several human cell lines, including A-431, COLO205, HepG2, HeLa, and HEK293 lysates, confirming reliable performance in routine protein expression studies. For cellular localization experiments, immunofluorescence staining has been validated in A-431 cells, while flow cytometry analysis in BxPC-3 cells enables quantitative assessment of RYK expression at the single-cell level.

The antibody is supplied in a stabilized liquid format with glycerol for convenient long-term storage at -20°C or -80°C. Recommended working dilutions range from 1:500-1:2000 for western blotting and 1:50-1:200 for immunofluorescence and flow cytometry applications.

This antibody serves researchers exploring Wnt signaling mechanisms, developmental processes, and oncology studies where understanding RYK-mediated pathways may reveal new therapeutic insights.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.

Gene References into Functions

our findings demonstrate that Ryk promotes stem cell-like and tumorigenic features to glioma cells its essential for the maintenance of glioblastoma stem cells PMID: 28086236
Wnt5A/Ryk signaling might provide novel therapeutic strategies to prevent capillary leakage in systemic inflammation and septic shock. PMID: 27159116
results corroborated previous findings of Ryk-mediated Wnt5a effect, and suggested a role for Ror2 in the Wnt5a machinery in glioblastoma PMID: 26596412
Early treatment with HGF/IgG complexes after myocardial ischaemia with reperfusion may rescue tissue through vasoprotection conferred by c-Met and RYK signalling PMID: 26025956
Ryk-intracellular domain pathway may impair FOXO protective activity in mutant polyglutamine neurons, suggesting neurons unable to efficiently maintain function and resist disease from the earliest phases of the pathogenic process in Huntington disease PMID: 24960609
is a candidate wnt5a receptor. PMID: 23748645
The Ryk is important for the Wnt-5a-dependent induction of MMP-2 and invasive activity in glioma-derived cells and that Ryk might have a novel patho-physiological function in adult cancer invasion. PMID: 24621529
RYK and VANGL2 proteins form a complex, whereas RYK also activated RhoA, a downstream effector of PCP signaling. PMID: 22773843
RYK interacts both physically and functionally with the E3 ubiquitin MIB1. MIB1 is sufficient to activate Wnt/CTNNB1 signaling and this activity depends on endogenous RYK. PMID: 21875946
RYK, a catalytically inactive receptor tyrosine kinase, associates with EphB2 and EphB3 but does not interact with AF-6. PMID: 11956217
a missense mutation, 1355G>A, and one rare single nucleotide polymorphisms haplotype may play a role in the development of cleft lip and/or palate in the Vietnamese, and cleft lip and/ or palate and cleft palate only in the Japanese PMID: 16681403
validated occurrence of an unusual TG 3' splice site in intron 7 PMID: 17672918

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Subcellular Location

Membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm.

Protein Families

Protein kinase superfamily, Tyr protein kinase family

Tissue Specificity

Observed in all the tissues examined.

Database Links

HGNC: 10481

OMIM: 600524

KEGG: hsa:6259

STRING: 9606.ENSP00000296084

UniGene: Hs.654562

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Inhibitors of axonal regeneration

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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