PPP2CB Antibody

Western blot
All lanes: Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform Antibody at 2μg/ml + EC109 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00985A0Rb at dilution of 1:50

Immunofluorescent analysis of Hela cells using CSB-PA00985A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

IHC image of CSB-PA00985A0Rb diluted at 1:260 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA00985A0Rb diluted at 1:260 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA00985A0Rb at dilution of 1:100