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Code | CSB-PA020641LA01RA |
Size | US$299 How to order? |
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Full Product Name | Rabbit anti-Rattus norvegicus (Rat) S100a8 Polyclonal antibody | ||||
Uniprot No. | P50115 | ||||
Target Names | S100a8 | ||||
Alternative Names | S100a8 antibody; Mrp8 antibody; Protein S100-A8 antibody; Calgranulin-A antibody; Migration inhibitory factor-related protein 8 antibody; MRP-8 antibody; p8 antibody; S100 calcium-binding protein A8 antibody | ||||
Raised in | Rabbit | ||||
Species Reactivity | Rat | ||||
Immunogen | Recombinant Rat Protein S100-A8 protein (2-89AA) | ||||
Immunogen Species | Rattus norvegicus (Rat) | ||||
Conjugate | Non-conjugated The S100a8 Antibody (Product code: CSB-PA020641LA01RA) is Non-conjugated. For S100a8 Antibody with conjugates, please check the following table. |
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Clonality | Polyclonal | ||||
Isotype | IgG | ||||
Purification Method | >95%, Protein G purified | ||||
Concentration | It differs from different batches. Please contact us to confirm it. | ||||
Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
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Form | Liquid | ||||
Tested Applications | ELISA, WB | ||||
Recommended Dilution |
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Protocols | ELISA Protocol Western Blotting(WB) Protocol |
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Troubleshooting and FAQs | Antibody FAQs | ||||
Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | ||||
Lead Time | Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
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Function |
S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. The iNOS-S100A8/A9 transnitrosylase complex is proposed to direct selective inflammatory stimulus-dependent S-nitrosylation of multiple targets such as GAPDH, ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif; S100A8 seems to contribute to S-nitrosylation site selectivity.
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Gene References into Functions |
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Subcellular Location | Secreted. Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein. |
Protein Families | S-100 family |
Database Links |
KEGG: rno:116547 STRING: 10116.ENSRNOP00000015473 UniGene: Rn.31839 |