SLC25A6 Antibody

Code CSB-PA021520LA01HU
Size US$299
Image
  • Western Blot
    Positive WB detected in: LO2 whole cell lysate, MCF-7 whole cell lysate, HEK293 whole cell lysate
    All lanes: SLC25A6 antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 33 kDa
    Observed band size: 33 kDa

  • IHC image of CSB-PA021520LA01HU diluted at 1:1200 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA021520LA01HU diluted at 1:1200 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) SLC25A6 Polyclonal antibody
Uniprot No. P12236
Target Names SLC25A6
Alternative Names SLC25A6; AAC3; ANT3; CDABP0051; ADP/ATP translocase 3; ADP,ATP carrier protein 3; ADP,ATP carrier protein, isoform T2; ANT 2; Adenine nucleotide translocator 3; ANT 3; Solute carrier family 25 member 6
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human ADP/ATP translocase 3 protein (144-175AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated

The SLC25A6 Antibody (Product code: CSB-PA021520LA01HU) is Non-conjugated. For SLC25A6 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA021520LB01HU SLC25A6 Antibody, HRP conjugated ELISA
FITC CSB-PA021520LC01HU SLC25A6 Antibody, FITC conjugated
Biotin CSB-PA021520LD01HU SLC25A6 Antibody, Biotin conjugated ELISA
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:1000-1:2000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane. In addition to its ADP:ATP antiporter activity, also involved in mitochondrial uncoupling and mitochondrial permeability transition pore (mPTP) activity. Plays a role in mitochondrial uncoupling by acting as a proton transporter: proton transport uncouples the proton flows via the electron transport chain and ATP synthase to reduce the efficiency of ATP production and cause mitochondrial thermogenesis. Proton transporter activity is inhibited by ADP:ATP antiporter activity, suggesting that SLC25A6/ANT3 acts as a master regulator of mitochondrial energy output by maintaining a delicate balance between ATP production (ADP:ATP antiporter activity) and thermogenesis (proton transporter activity). Proton transporter activity requires free fatty acids as cofactor, but does not transport it. Also plays a key role in mPTP opening, a non-specific pore that enables free passage of the mitochondrial membranes to solutes of up to 1.5 kDa, and which contributes to cell death. It is however unclear if SLC25A6/ANT3 constitutes a pore-forming component of mPTP or regulates it.
Gene References into Functions
  1. Human ANT3 expression is directly downregulated by overexpression of hcmv-miR-UL36-5p. PMID: 26212361
  2. The dynamics of the molecular interactions between the C-terminal region of PB1-F2 protein and VDAC1 and ANT3 were expounded by employing an in silico approach. PMID: 19669810
  3. Of three ANT isoforms expressed in yeast, ANC3 is the most efficient to restore yeast growth on a nonfermentable carbon source of a yeast mutant strain lacking its three endogenous ANC. PMID: 12450408
  4. Results demonstrate that induction of adenine nucleotide translocase 3 by interleukin-4 and interferon-gamma proceeds via pathways involving STAT6 and STAT1, respectively. PMID: 14746803
  5. ANT3 overexpression induces apoptosis in cultured cells. PMID: 15063741
  6. ANT3 has a potential role in T-helper cell survival and immune cell homeostasis. PMID: 16930576
  7. involvement of ANT in cell death is cell type- and stimulus-dependent PMID: 17855512
  8. These data suggest that the down-regulation of ANT3 levels upon stimulation with CPT may be involved in the molecular mechanism underlying acquired resistance to camptothecin. PMID: 19111545

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Subcellular Location Mitochondrion inner membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein.
Protein Families Mitochondrial carrier (TC 2.A.29) family
Tissue Specificity Expressed in erythrocytes (at protein level).
Database Links

HGNC: 10992

OMIM: 300151

KEGG: hsa:293

STRING: 9606.ENSP00000370808

UniGene: Hs.350927

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