SLC6A3 Antibody

Code CSB-PA021701LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
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  • Western Blot
    Positive WB detected in: Mouse brain tissue
    All lanes: SLC6A3 antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 69 kDa
    Observed band size: 69 kDa

  • Immunofluorescent analysis of HepG2 cells using CSB-PA021701LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) SLC6A3 Polyclonal antibody
Uniprot No. Q01959
Target Names SLC6A3
Alternative Names DA transporter antibody; DAT 1 antibody; DAT antibody; DAT1 antibody; Dopamine transporter 1 antibody; Dopamine transporter antibody; PKDYS antibody; SC6A3_HUMAN antibody; SLC6A3 antibody; Sodium dependent dopamine transporter antibody; Sodium-dependent dopamine transporter antibody; Solute carrier family 6 (neurotransmitter transporter dopamine), member 3 antibody; Solute carrier family 6 (neurotransmitter transporter), member 3 antibody; Solute carrier family 6 member 3 antibody; Variable number tandem repeat (VNTR) antibody
Raised in Rabbit
Species Reactivity Human, Mouse
Immunogen Recombinant Human Sodium-dependent dopamine transporter protein (161-237AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IF
Recommended Dilution
Application Recommended Dilution
WB 1:2000-1:10000
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.
Gene References into Functions
  1. genetically determined differences in DAT1 and DRD2 expression modulate functional consequences of sleep deprivation. PMID: 28393838
  2. SLC6A3 gene moderates the relation between maternal history of maltreatment and infant emotion regulation. PMID: 28803562
  3. The age-related reduction in striatal DAT density also predicted memory decline, suggesting that a relation between striatal functions and memory decline in aging is multifaceted. PMID: 30224467
  4. genetic association studies in population of young adults in Columbia: Data suggest that genetic polymorphisms in DRD4 and SLC6A3 are associated with overweight/obesity in the population studied; DRD4 4/4 genotype is associated with lower BMI and SLC6A3 10/10 genotype is associated with higher BMI. (DRD4 = dopamine receptor D4; SLC6A3 = solute carrier family 6 member 3) PMID: 29145734
  5. Cloninger's (1987) hypothesis about negative relationship between novelty seeking and dopamine was confirmed on allele level, because higher novelty seeking was found in Val allele carriers comparing to Met/Met genotype carriers. PMID: 29546858
  6. Results indicate that chronic coffee consumption in Parkinson's disease (PD) patients was not associated with significant change in striatal DAT availability, even after taking into account potentially confounding factors that are probably related to dopaminergic neuron decline (age, sex, disease duration, and severity of PD), treatment, and coffee-related habit (cigarette smoking). PMID: 29362953
  7. This is the first study to investigate the association of rs3910105 in SNCA [SNCA protein, human] with DAT [dopamine transporter] availability. rs3910105 had an effect on DAT availability, and the correlation between DAT availability and SNCA transcripts were significant in CT [computed tomography] genotypes of rs3910105. PMID: 29978616
  8. Study describes an association between the 6R6R genotype of the intron 8 VNTR of the dopamine transporter (DAT1) gene and crack cocaine addiction. PMID: 29332099
  9. None of the polymorphisms studied showed a reliable association with response inhibition performance. The methodological and theoretical implications of these findings are discussed. PMID: 29155230
  10. From all the statistically significant CpGs, methylation levels of cg00997378 (SLC6A3 gene) showed the highest differences (p < 0.0001), being associated with prematurity risk factors. SLC6A3 methylation, previously related to attention-deficit/hyperactivity disorder, neuronal function and behavior, might be a potential epigenetic biomarker with value in the early diagnosis and management of neurodevelopmenta PMID: 29172706
  11. Results show that Gbetagamma activation regulates DAT activity by increasing dopamine (DA) efflux, and suggests that Gbetagamma promotes an efflux-willing state of the transporter and that the activation of a GPCR can lead to a Gbetagamma-dependent DA efflux. Furthermore, the cellular responses that occur following activation of a Galphaq-coupled receptor are the result of multiple signaling pathways mediated by Galpha... PMID: 28894302
  12. Findings demonstrate that histidine547 on hDAT plays a crucial role in stabilizing basal dopamine transport and Tat-DAT interaction. PMID: 27966610
  13. In a study of the genetic association between polymorphisms in the DAT1, SERT, COMT and BDNF genes and attention deficit and hyperactive disorder, transmission disequilibrium test analysis showed that no individual allele of any variant studied has a preferential transmission. PMID: 29122229
  14. SLC6A3 repeat allele was higher proportion in Indonesian children with ADHD than healthy controls. PMID: 29061424
  15. DAT1 genotype and motor threshold interact on prepulse facilitation following brain stimulation. PMID: 28337537
  16. This study demonstrate the genetic influence of a family history of alcohol use disorders and DAT and DBH gene polymorphisms on the risk of withdrawal seizures and delirium tremens. PMID: 28139629
  17. Multilevel models revealed that history of care had a greater influence on maternal baseline cortisol(but not cortisol trajectory)for mothers with more plasticity alleles of SLC6A3 and OXTR, relative to mothers with fewer or no plasticity alleles. Findings indicate that a mother's history of care is related to her cortisol secretion in anticipation of infant stress, but this relation depends on her genetic characteristics PMID: 28890000
  18. This combined computational-experimental study demonstrates that histidine-547 (H547) of human DAT plays a crucial role in the DAT-HIV-1 Tat binding and dopamine uptake by DAT. PMID: 27250920
  19. This results of this study showed the evidence that being born SGA moderates the effect of SNPs in DAT1 (a gene previously associated with ADHD) on the risk for ADHD symptoms. PMID: 28666839
  20. Findings suggest that responses to attention-grabbing features in smoking-cessation messages is affected by the DAT1 genotype. PMID: 28757070
  21. The subjective responses to alcohol consumption in social drinkers were associated with significant epistatic interactions between OPRM1 and DAT1 genotypes. PMID: 28376280
  22. Epigenetic alterations of DAT1 in modulating the response to methylphenidate in ADHD, primarily on oppositional and hyperactive-impulsive symptoms. PMID: 27676100
  23. Studied neural activation during an emotional response inhibition task across different DAT1 genotypes in healthy individuals, showed that genotypic variation influencing basal dopamine levels can alter the neural basis of emotional processing and response inhibition. This may indicate that 9R carriers exert more effort to overcome increased basal dopamine activation when inhibiting responses in emotional contexts. PMID: 26843006
  24. The current study, the effect of the dopamine transporter gene DAT1/SLC6A3 on striatal brain volume was investigated in children and adults with ADHD and healthy participants in three different cross-sectional cohorts. PMID: 26935821
  25. Study analyzed effects of DRD4 and DAT1, prenatal exposure to alcohol and smoking and interactions on ADHD severity, response inhibition and neural activity. DRD4 7-repeat allele associated with less superior frontal and parietal activity & greater activity in frontal pole and occipital cortex. Alcohol-exposed had more activity in lateral orbitofrontal cortex, & DAT1 risk variant associated with lower cerebellar activity. PMID: 28234207
  26. We investigated genetic associations with reflexive attention measures in infancy and childhood in the same group of children. Performance on the infant task was associated with SLC6A3. In addition, several genetic associations with an analogous child task occurred with markers on CHRNA4, COMT, and DRD4. PMID: 26613685
  27. Linear regression analyses demonstrated that the increasing number of COMT158Val and DAT1 10R alleles significantly predicted increasing hyperactivity/inattention in boys at both 11 and 15 years of age. Presence of both COMT158Val and DAT1 10R alleles was also associated with full categorical attention-deficit/hyperactivity disorder diagnosis at 18 years of age in boys. Study did not observe these associations in girls. PMID: 27327562
  28. Results are consistent with the suggested associations between DAT1 and risk-taking behavior. The counter-intuitive finding for partner selection and treatment adherence may be evidence of additional factors that place PLH at risk for engaging in hazardous drinking as well as relationship difficulties and problems with treatment adherence (e.g., depressive symptoms, avoidant coping, trauma history). PMID: 28278565
  29. Unsubstituted amphetamine-like cathinones bind more favorably to DAT, due to a Val152 offering more space, as compared to the bulkier Ile172 in SERT. This was supported by uptake inhibition measurements, which showed an increase in activity in SERT-I172V. PMID: 27860344
  30. concluded that the Variable Number Tandem Repeats polymorphism has an important role in the etiology of Alcohol Dependence, and individuals with at least 1 A9 allele are more likely to be dependent on alcohol than persons carrying the non-A9 allele. PMID: 27219321
  31. Striatal DAT and diencephalic SERT binding negatively correlated with food detection speed, but not with food distraction time, ratings of hunger, craving or impulsivity. Striatal DAT and diencephalic SERT binding did not correlate with free choice food intake. PMID: 26984412
  32. Participants carrying a copy of the nine-repeat DAT allele-linked to higher phasic dopamine activity-expressed amplified striatal response during anticipation of monetary gain following sleep deprivation. Moreover, participants homozygous for the ten-repeat DAT allele-linked to lower phasic dopamine activity-selectively demonstrated an increase in sensitivity to monetary loss within anterior insula following sleep loss. PMID: 26918589
  33. SLC6A3 gene variants have been reported to be implicated in alcohol addiction, nicotine dependence and other addictive behaviors. PMID: 27490263
  34. The 3' VNTR polymorphism affects human DAT expression level in iPSC-derived human dopaminergic neurons. PMID: 27547496
  35. Study measured smooth pursuit in 110 healthy subjects genotyped for two well-documented polymorphisms, the COMT Val(158)Met polymorphism and the SLC6A3 3'-UTR-VNTR polymorphism; modulation of striatal dopamine activity by the SLC6A3 3'-UTR-VNTR polymorphism had no significant functional effect. PMID: 28101524
  36. Allosteric modulation of human dopamine transporter activity under conditions promoting its dimerization PMID: 28584050
  37. Maternal ADHD symptomatology, maternal DAT1, and home atmosphere contribute to boys' ADHD Symptomatology at 7 years of age. PMID: 27873141
  38. A combined haplotype (9R-A2) of DAT-1 and DRD2 genes was associated with the murderer phenotype among Pakistani violent criminal inmates. PMID: 28582390
  39. DAT1-10R carriers fared the same as 9R homozygotes in poorer and moderate school environments, but fared disproportionately better in more positive environments. PMID: 27786532
  40. SLC6A3 gene may have a role in susceptibility to late-onset alcohol dependence in the Han Chinese population. PMID: 28182634
  41. Low school engagement in adolescence is more pronounced for DAT1-10R homozygotes. PMID: 27268567
  42. In children with ADHD, multiple mediation analysis revealed significant indirect associations between DAT1 genotype and inattention, hyperactivity-impulsivity, and oppositionality, with specific indirect effects through response inhibition. PMID: 27049476
  43. This result suggests that persistent drug-induced parkinsonism in patients with visually normal DAT imaging may be associated with subtle decrement of DAT activity. PMID: 27294367
  44. the dopamine transporter SLC6A3 constitutes a novel biomarker that is highly specific for clear cell renal cell carcinoma PMID: 27663598
  45. Data suggest that variants rs28363170 and rs3836790 in the solute carrier family 6 member 3 gene are not associated with sporadic Parkinson's disease in Han Chinese population. PMID: 27353511
  46. Study systematically designed and characterized a set of 24 substrate-based bivalent ligands, and in combination with mutagenesis, induced-fit docking calculations, electrophysiology, and cysteine accessibility measurements; revealed novel insight into the molecular basis of substrate recognition in hSERT and hDAT PMID: 27425420
  47. these findings provide compelling experimental evidence that DAT N and C termini synergistically contribute to substrate and inhibitor affinities. PMID: 27986813
  48. these results are the first demonstration of pharmacological chaperoning of DAT and suggest this may be a viable approach to increase DAT levels in Dopamine Transporter Deficiency Syndrome and other conditions associated with reduced DAT function. PMID: 27555326
  49. total of 1799 differentially methylated regions were identified including SLC6A3, Rab40C, ZNF584, and FOXD3 whose significant methylation differences were confirmed in breast cancer patients through quantitative real-time polymerase chain reaction.Methylation of those aforementioned genes in white blood cells of our young patients may highlight their potential as early epimarkers PMID: 28349825
  50. Data indicate genetic association between dopamine transporter SLC6A3 (DAT1) and hypertension, and the specific variable number tandem repeats (VNTR) alleles of SLC6A3 may be a risk factor for hypertension. PMID: 28055236
  51. The effects of parenting on self-control and offending are most pronounced for those who carry plasticity alleles for both MAOA and DAT1. Thus, MAOA and DAT1 may be implicated in offending because they increase the negative effects of parenting on self-control. Implications for theory are discussed. PMID: 25326468
  52. DAT1 10-repeat homozygotes with ADHD showed the least reward speeding when exposed to high positive peer affiliation, but the most when faced with low positive peer affiliation or low maternal warmth. PMID: 27045841
  53. The presence of particular variants of variable number tandem repeat polymorphisms in 3' untranslated region of SLC6A3 gene in men is associated with predisposition to certain forms of antisocial behavior. PMID: 27882466
  54. The current study reanalyzed the Add Health genetic subsample to assess the relative effects of these dopaminergic genes and self-control on GPAs. The results demonstrated that academic performance fully mediated the linkage between these genes and violent delinquency. PMID: 25326467
  55. Increased nigral SLC6A3 activity may be a risk factor for SCZ. PMID: 26707863
  56. Findings suggest that adverse intrauterine environments leading to low birth weight also seem to exacerbate the effects of certain DAT1 variants on depression. PMID: 26991370
  57. The 10R allele as a DAT1 gene VNTR polymorphism might be related to methylphenidate-associated changes in brain metabolites in attention deficit hyperactivity disorder patients. PMID: 27097961
  58. DRD4 and SLC6A3 gene polymorphisms may have a role in food intake and nutritional status in children in early stages of development PMID: 26350252
  59. Our findings suggest that rare high-risk copy number variations affecting the gene encoding the dopamine transporter contribute to the pathogenesis of schizophrenia and affective disorders. PMID: 26559242
  60. Compared with healthy controls, opioid-dependent individuals showed a significant reduction in striatal DAT density, which also negatively correlated with non-perseverative errors in working memory. PMID: 25439653
  61. Study failed to observe a significant association of the SLC6A3 40-base pair variable number of tandem repeats polymorphism (rs28363170) with cognitive function in healthy adults PMID: 26593110
  62. the 3'-UTR VNTR polymorphism is significantly associated with smoking cessation, and smokers with one or more 9-repeat alleles have a 17% higher probability of smoking cessation than smokers carrying no such allele. [meta-analysis] PMID: 26149737
  63. high SLC6A3 expression in Clear cell renal cell carcinoma tissue was correlated with a shorter period of recurrence-free survival following surgery. PMID: 26831905
  64. Children without the haplotypes associated with the reduced SLC6A3 gene expression and thus lower dopamine functioning appeared to demonstrate altered levels of effortful control as a function of maternal parenting quality. PMID: 25924976
  65. Computational study providse novel insights into the role of collective dynamics in functionally relevant state-to-state transitions in DAT, at a level of atomistic detail PMID: 26255829
  66. No significant association between candidate gene variants and criminal behavior was detected. PMID: 26041607
  67. Higher levels of maternal depressive symptoms predicted reduced cortisol flexibility across challenges for infants with at least one A1 allele of DRD2 and infants with the 10/10 genotype of SLC6A3 PMID: 26342565
  68. 3'-UTR variable number tandem repeat associated with post-traumatic stress disorder [meta-analysis] PMID: 26986136
  69. The NET and DAT N Termini Are Critical for Internalization and Postendocytic Sorting. PMID: 26786096
  70. the putative involvement of DAT1 and DRD4 genes in the aetiology of Attention-deficit hyperactivity disorder with a main role in modulation of key dimensions of the disorder PMID: 25555995
  71. Functional polymorphisms of the genes encoding adenosine A2A receptors and dopamine transporters contribute to individual differences in impaired sleep quality by caffeine. PMID: 27167478
  72. DAT1 plays a role in the neurobiology of response inhibition. PMID: 26073896
  73. DAT1 variants are associated with reaction time slops on a reflexive attention task. PMID: 26471374
  74. we did not find any significant associations of COMT or SLC6A3 with inhibitory performance or impulsivity PMID: 26245713
  75. This study reveals that a common genetic variant in DAT1, previously linked with the clinical stages of dementia in humans, and with increased DAT expression in vitro, is associated with several brain-related health factors, including diagnostic, cognitive, and anatomical indices of neurodegeneration, providing new insight into the pathogenesis of Alzheimer's disease PMID: 25496873
  76. Our study suggests that DJ-1 may also play a role in regulating dopamine levels by modifying DAT activity. PMID: 26305376
  77. results showed that the methylation status of the BDNF and DAT1 genes had no significant function in the processes of drug addiction PMID: 26160290
  78. that the cdc42-activated, nonreceptor tyrosine kinase, Ack1, is a DAT endocytic brake that stabilizes DAT at the plasma membrane. PMID: 26621748
  79. Zn(2+) dissociated more rapidly from the inward- than from the outward-facing state of DAT. PMID: 26504078
  80. the majority of DAT.alpha-synuclein protein complexes are found at the plasma membrane of dopaminergic neurons or mammalian cells PMID: 26442590
  81. Apathy is associated with reduced striatal dopamine transporter levels in Parkinson's disease patients. PMID: 25753457
  82. Dynamical analysis of attention/inhibition measures may unravel new correlates to DRD4 and SLC6A3 variants PMID: 22930791
  83. these data identify the relative contribution of DNMTs and HDACs in regulating expression of dopamine transporter PMID: 25963949
  84. DAT1 10R carriers showed more rule-breaking behavior according to parents when experiencing high peer rejection, but less rule-breaking behavior when experiencing low peer rejection PMID: 26006708
  85. The cortical thickness of right lateral prefrontal cortex is influenced by the presence of the DAT1 10 repeat allele in children and adolescents with ADHD. PMID: 26206710
  86. Reductions in dopamine transporter availability may play a pathophysiological role in the development of pure alcohol dependence, given its association with neurocognitive deficits. PMID: 26120847
  87. variations in the DAT1 gene may influence the amount of Dopamine availability and the experience of reward in both Substance use disorders and compulsive overeating. PMID: 26478196
  88. Compared with healthy controls, significant reduction in dopamine transporter availability was found in heroin users at baseline in both the right (by approximately 31.6%) and left striatum. PMID: 26096461
  89. A correlation was found between DAT1 VNTR and the subtest "processing speed index" of WISC and the subtest "cognitive flexibility" of executive functions. First to assess DAT1 gene in Colombian population. PMID: 24461309
  90. As a conclusion, there was no association between 5-HTT, DAT1 and DRD4 genes VNTR polymorphisms and obesity PMID: 25687785
  91. Children with OFC were significantly less likely to have the DAT1 10-repeat allele (p = .04). PMID: 25271118
  92. The SLC6A3/DAT1 40 bp-VNTR may affect life expectancy in a sex-specific way. PMID: 25617181
  93. This study demonstrated that The bipolar disorder patients had a higher rate of harm avoidance personality traits than the controls, and DAT1 variants may influence personality traits in patients with bipolar disorder. PMID: 25547317
  94. The data presented here show for the first time support for the hypothesis that genetic differences in DAT1 contribute to the variation in subjective responses to cocaine among participants with cocaine use disorders PMID: 25850966
  95. No statistical differences in the general frequency of DAT CpG islands was revealed between patients and control subjects. PMID: 25862379
  96. Results provide dynamic imaging of single DAT proteins, providing new insights into the relationship between surface dynamics and trafficking of both wild-type and attention deficit/hyperactivity disorder-associated transporters PMID: 25747272
  97. Study found that DAT1 variability did not have any effects on metacontrast masking PMID: 25804664
  98. This study shown DAT1 to be the risk factor for attention deficit/hyperactivity disorder. PMID: 25840828
  99. We have found an association between 40 bp VNTR polymorphism of DAT1/SLC6A3 gene and ADHD in the Czech population PMID: 26058807
  100. Study developed a three-dimensional structural model for HIV-1 Tat binding with hDAT, providing novel mechanistic insights concerning how HIV-1 Tat interacts with hDAT and inhibits dopamine uptake by hDAT PMID: 25695767

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Involvement in disease Parkinsonism-dystonia infantile (PKDYS)
Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families Sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family, SLC6A3 subfamily
Tissue Specificity Highly expressed in substantia nigra.
Database Links

HGNC: 11049

OMIM: 126455

KEGG: hsa:6531

STRING: 9606.ENSP00000270349

UniGene: Hs.406

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