SMAD1 Antibody

Datasheet

Code CSB-PA618998LA01HU
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Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. Q15797
Image
  • Western blot
    All lanes: SMAD1 antibody at 4 ug/ml
    Lane 1: HepG2 whole cell lysate
    Lane 2: Mouse skeletal muscle tissue
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 53, 16 kDa
    Observed band size: 47 kDa

  • Western blot
    All lanes?:?SMAD1 antibody at 6 ug/ml
    Lane 1?: Jurkat whole cell lysate
    Lane 2?: Hela whole cell lysate
    Lane 3?: HepG2 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 53, 16 kDa
    Observed band size: 52, 37 kDa

  • Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA618998LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA618998LA01HU at dilution of 1:100

  • Western Blot
    Positive WB detected in:Hela whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate
    All lanes:SMAD1 antibody at 3ug/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 53 kDa
    Observed band size: 53 kDa

  • IHC image of CSB-PA618998LA01HU diluted at 1:600 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells with CSB-PA618998LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunogen Recombinant human Mothers against decapentaplegic homolog 1 protein (202-335AA)
Raised in Rabbit
Species Reactivity Human, Mouse
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:600, IF:1:50-1:200
Relevance Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Mothers against decapentaplegic homolog 1 (MAD homolog 1) (Mothers against DPP homolog 1) (JV4-1) (Mad-related protein 1) (SMAD family member 1) (SMAD 1) (Smad1) (hSMAD1) (Transforming growth factor-beta-signaling protein 1) (BSP-1), SMAD1, BSP1 MADH1 MADR1
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Research Area Signal Transduction
Target Names SMAD1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.
Involvement in disease SMAD1 variants may be associated with susceptibility to pulmonary hypertension, a disorder characterized by plexiform lesions of proliferating endothelial cells in pulmonary arterioles. The lesions lead to elevated pulmonary arterial pression, right ventricular failure, and death. The disease can occur from infancy throughout life and it has a mean age at onset of 36 years. Penetrance is reduced. Although familial pulmonary hypertension is rare, cases secondary to known etiologies are more common and include those associated with the appetite-suppressant drugs.
Subcellular Location Cytoplasm, Nucleus
Protein Families Dwarfin/SMAD family
Tissue Specificity Ubiquitous. Highest expression seen in the heart and skeletal muscle.
Database Links

HGNC: 6767

OMIM: 601595

KEGG: hsa:4086

STRING: 9606.ENSP00000305769

UniGene: Hs.604588

Pathway Hippo signaling pathway
TGF-beta signaling pathway
Signaling pathways regulating pluripotency of stem cells

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