Product Details

Uniprot No.

Target Names

Alternative Names

Mothers against decapentaplegic homolog 1 (MAD homolog 1) (Mothers against DPP homolog 1) (JV4-1) (Mad-related protein 1) (SMAD family member 1) (SMAD 1) (Smad1) (hSMAD1) (Transforming growth factor-beta-signaling protein 1) (BSP-1), SMAD1, BSP1 MADH1 MADR1

Species Reactivity

Human

Immunogen

A synthesized peptide from human SMAD1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8G10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SMAD1 serves as a critical intracellular signal transducer in the bone morphogenetic protein (BMP) signaling pathway, playing essential roles in embryonic development, cell differentiation, and tissue homeostasis. As a receptor-regulated SMAD, it becomes phosphorylated upon BMP receptor activation and subsequently translocates to the nucleus to regulate target gene expression. This central position in BMP signaling makes SMAD1 a key target for researchers investigating developmental biology, stem cell differentiation, bone formation, and various disease processes including cancer and fibrosis.

This recombinant monoclonal antibody, clone 8G10, offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide from human SMAD1, the recombinant production method ensures sequence-defined specificity and eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations.

Validation across multiple applications demonstrates this antibody's experimental flexibility. Western blot analysis successfully detects SMAD1 in U-87MG glioblastoma, A549 lung carcinoma, and HEK293 cell lysates, with an observed band at approximately 50 kDa. The slight difference from the predicted 52 kDa molecular weight likely reflects post-translational processing or variations in gel migration conditions. Immunofluorescence staining in HeLa cells reveals clear intracellular localization patterns, while flow cytometry analysis in Jurkat cells shows distinct positive signal separation from isotype controls, confirming utility for quantitative single-cell analyses.

Whether investigating BMP-mediated differentiation programs, characterizing SMAD1 expression across cell populations, or examining subcellular localization dynamics, this antibody provides researchers with a reliable tool for exploring this fundamental signaling pathway across diverse experimental platforms.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.

Gene References into Functions

miR-26a-5p is highly expressed in synovial tissue of patients with RA, and its high expression can improve the invasive ability of synovial fibroblasts by targeting Smad 1 gene and accelerating the progression of RA. PMID: 30046030
miR-23a facilitated cell proliferation and migration by targeting BMPR2/Smad1 signaling in hypoxia-induced human pulmonary artery smooth muscle cells. PMID: 29864909
the expression of BMP15 in follicular fluid and Smad1 in granulosa cells was significantly decreased in the PCOS group compared with the control (P<0.05). The data suggested that the BMP15/Smad1 signalling pathway may be involved in granulosa cell apoptosis PMID: 28983616
Mechanical stress affects the osteogenic differentiation of human ligamentum flavum cells via the BMP-Smad1 signaling pathway. PMID: 28944874
Urinary Smad1 was associated with the degree of mesangial expansion in early diabetic nephropathy. PMID: 29490904
Differential expression of TGF-beta superfamily members and role of Smad1/5/9-signalling in chondral versus endochondral chondrocyte differentiation. PMID: 27848974
Uev1A appears to be involved in the BMP signaling pathway in which it collaborates with a ubiquitin E3 ligase Smurf1 to promote Smad1 degradation in a Ubc13-independent manner. PMID: 28771228
Data show that miR-26b-5p suppresses Twist1-induced EMT, invasion, and metastasis of HCC cells by targeting SMAD1. PMID: 27027434
Testosterone promoted tube formation of human umbilical endothelial cells, which was blocked by c-Src and ERK1/2 inhibitors or by the knockdown of Smad1. PMID: 28167128
Low doses of IL1B activate the BMP/Smad signaling pathway to promote the osteogenesis of periodontal ligament stem cells, but higher doses of IL1B inhibit BMP/Smad signaling through the activation of NF-kappaB and MAPK signaling, inhibiting osteogenesis. PMID: 27415426
Store operated calcium entry negatively regulates the Smad1 signaling pathway and inhibits Col IV protein production in glomerular mesangial cells. PMID: 28298362
A significant association was found between the low expression of inhibitory protein SMAD-7 and both zeta-chain-associated protein kinase 70-negative cells (p = 0.04) and lower apoptotic index (p = 0.004). No differences were observed in SMAD-2/3 expression. In conclusion, our results demonstrate a significant correlation between greater SMAD-1/8 and lower SMAD-4 expression in chronic lymphocytic leukemia cells PMID: 28349818
melatonin treatment was found to downregulate TNFalpha-induced SMURF1 expression and then decrease SMURF1-mediated ubiquitination and degradation of SMAD1 protein PMID: 27265199
The expression of specific targets Smad1 and Osterix was significantly increased in the presence of Pi and restored by coincubation with Mg(2+). As miR-30b, miR-133a, and miR-143 are negatively regulated by Pi and restored by Mg(2+) with a congruent modulation of their known targets Runx2, Smad1, and Osterix, our results provide a potential mechanistic explanation of the observed upregulation of these master switches of o PMID: 27419135
the BMP-2/Smad1/5/RUNX2 signaling pathway participates in the silicon-mediated induction of COL-1 and osteocalcin synth PMID: 27025722
Regulation of impaired angiogenesis in diabetic dermal wound healing by microRNA-26a is mediated by the increased expression of its target gene, SMAD1. PMID: 26776318
The expression SMAD1 protein showed a significant correlation with lung cancer differentiation and lymphatic metastasis (P < 0.05), but not with genders, ages, tumor sizes and histological types of lung cancer patients (P>0.05). PMID: 27049088
Overexpression of Smad1 is associated with prostate cancer. PMID: 26227059
SMAD1 signaling may be a key pathway contributing the pathogenesis of Cardio-facio-cutaneous syndrome during early development. PMID: 25639853
Smad1 elevation serves as a compensatory mechanism for p53 deficiency by potentiating the activation of p53 parallel pathways. PMID: 25757624
Our data indicated that downregulation of miR-26b in osteosarcoma elevated the levels of CTGF and Smad1, facilitating osteosarcoma metastasis PMID: 25761878
Smad1 as a novel binding protein of KSHV latency-associated nuclear antigen (LANA). LANA interacted with and sustained BMP-activated p-Smad1 in the nucleus and enhanced its loading on the Id promoters. PMID: 25010525
adult human Sertoli cells assumed similar morphological features, stable global gene expression profiles and numerous proteins, and activation of AKT and SMAD1/5 during long-period culture. PMID: 25880873
balance between Smad1/5- and Smad2/3-dependent signaling defines the outcome of the effect of TGF-beta on atherosclerosis where Smad1/5 is responsible for proatherogenic effects PMID: 25505291
Data show that USP15 enhances BMP-induced phosphorylation of SMAD1 by interacting with and deubiquitylating ALK3. PMID: 24850914
urinary Smad1 may be a potential diagnostic parameter for diabetic nephropathy and may be used to evaluate the severity of diabetic nephropathy PMID: 23943254
Inhibiting Smurf1 mediated ubiquitination of Smad1/5. PMID: 24828823
Smad1 is directly downregulated by miR-205. mRNA levels are not affected but Smad1 protein is decreased by miR-205 overexpression and increased by miR-205 inhibition. PMID: 23800974
Results indicate that BMP/Smad signaling pathway was altered during the period of osteogenesis, and that the activities of p-Smad1/5 were required for Saos-2 cells viability and differentiation induced by fluoride. PMID: 23918166
Glucocorticoids recruit Tgfbr3 and Smad1 to shift transforming growth factor-beta signaling from the Tgfbr1/Smad2/3 axis to the Acvrl1/Smad1 axis in lung fibroblasts. PMID: 24347165
the shear-induced apoptosis and autophagy are mediated by bone morphogenetic protein receptor type (BMPR)-IB, BMPR-specific Smad1 and Smad5, and p38 mitogen-activated protein kinase. PMID: 24021264
a detailed computational model for TGF-beta signalling that incorporates elements of previous models together with crosstalking between Smad1/5/8 and Smad2/3 channels through a negative feedback loop dependent on Smad7. PMID: 23804438
Data indicate a transcription complex androgen receptor (AR)-p44-Smad1, and confirmed for physical interaction by co-immunoprecipitaion. PMID: 23734213
our studies establish that loss of SMAD1/5 leads to upregulation of PDGFA in ovarian granulosa cells PMID: 22964636
Oscillatory shear stress induces synergistic interactions between specific BMPRs and integrin to activate Smad1/5 through the Shc/FAK/ERK pathway PMID: 23387849
shows role of ALK-1 in many process related to cardiovascular homeostasis, and the involvement of this protein in the development of cardiovascular diseases, suggesting the possibility of using the ALK-1/smad-1 pathway as a powerful therapeutic target PMID: 23707512
TNF activated NF-kappaB pathway and inhibited the phosphorylation of Smad 1/5/8 and BMP-2-induced osteoblastic differentiation in BMMSCs PMID: 22897816
Immunohistochemical analysis furthermore revealed that phosphorylated Smad1/5/8 and endoglin expression were significantly higher in high-grade compared to low-grade chondrosarcoma and correlated to each other. PMID: 23088614
Immunohistochemical analysis of phosphorylated Smad1 showed nuclear expression in 70% of the osteosarcoma samples at levels comparable to osteoblastoma. Cases with lower expression showed significantly worse disease free survival. PMID: 22868198
Cav-1 is required and sufficient for Smad1 signaling in human dermal fibroblasts. PMID: 22277251
Data suggest that Smads 1, 5 and 8 as potential prognostic markers and therapeutic targets for mTOR inhibition therapy of prostate cancer. PMID: 22452883
TGF-beta induces the formation of complexes comprising phosphorylated Smad1/5 and Smad3, which bind to BMP-responsive elements in vitro and in vivo and mediate TGF-beta-induced transcriptional repression. PMID: 22615489
Force-specific activation of Smad1/5 regulates vascular endothelial cell cycle progression in response to disturbed flow. PMID: 22550179
Smurf1 is a negative feedback regulator for IFN-gamma signaling by targeting STAT1 for ubiquitination and proteasomal degradation. PMID: 22474288
PAK2 negatively modulate TGF-beta signaling by attenuating the receptor-Smad interaction and thus Smad activation PMID: 22393057
BMP-9 induced endothelial cell tubule formation and inhibition of migration involves Smad1 driven endothelin-1 production. PMID: 22299030
Urinary Smad1 is a sensitive biomarker for diagnosis of diabetic glomerulosclerosis. PMID: 22073863
Results show that BMP4-induced changes in OvCa cell morphology and motility are Smad-dependent with shRNA targeting Smads 1, 4, and 5. PMID: 21945631
Expression of mutated Smad1 in adult human MSC cultures also resulted in increased nuclear accumulation of BMP-activated Smads and elevated gene transcripts characteristic of differentiating osteoblasts PMID: 21405981
Endoglin promotes fibrosis in scleroderma fibroblasts via TGF-beta/Smad1 signaling. PMID: 21344387

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Involvement in disease

SMAD1 variants may be associated with susceptibility to pulmonary hypertension, a disorder characterized by plexiform lesions of proliferating endothelial cells in pulmonary arterioles. The lesions lead to elevated pulmonary arterial pression, right ventricular failure, and death. The disease can occur from infancy throughout life and it has a mean age at onset of 36 years. Penetrance is reduced. Although familial pulmonary hypertension is rare, cases secondary to known etiologies are more common and include those associated with the appetite-suppressant drugs.

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Dwarfin/SMAD family

Tissue Specificity

Ubiquitous. Highest expression seen in the heart and skeletal muscle.

Database Links

HGNC: 6767

OMIM: 601595

KEGG: hsa:4086

STRING: 9606.ENSP00000305769

UniGene: Hs.604588

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Code	CSB-RA056935A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: U-87MG whole cell lysate(30µg), A549 whole cell lysate(30µg), HEK293 whole cell lysate(30µg) All lanes: SMAD1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 52 kDa Observed band size: 50 kDa Exposure time：2min40s Immunofluorescence staining of Hela cell with CSB-RA056935A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing jurkat cells stained with CSB-RA056935A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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SMAD1 Recombinant Monoclonal Antibody

Product Details

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Target Background