SOX2 Antibody

Datasheet
Code CSB-PA16539A0Rb
See More Details Free Antibody trial simple
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western blot
    All lanes: Transcription factor SOX-2 antibody at 2μg/ml + Mouse kidney tissue
    Secondary
    Goat polyclonal to rabbit IgG at 1/15000 dilution
    Predicted band size: 34 kDa
    Observed band size: 34 kDa

  • Western Blot
    All lanes: Transcription factor SOX-2 antibody at 6μg/ml + 293T whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/15000 dilution
    Predicted band size: 34 kDa
    Observed band size: 34 kDa

  • IHC image of CSB-PA16539A0Rb diluted at 1:500 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Western Blot
    Positive WB detected in: Mouse brain tissue
    All lanes: SOX2 antibody at 3.5μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 34 kDa
    Observed band size: 34 kDa

  • Western Blot
    Positive WB detected in: Zebrafish 40μg, 20μg, 10μg
    All lanes: Sox2 antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 35 kDa
    Observed band size: 35 kDa

  • IHC image of CSB-PA16539A0Rb diluted at 1:500 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-PA16539A0Rb at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Product Details

Full Product Name Rabbit anti-Human SOX2 Polyclonal antibody
Uniprot No. P48431
Target Names SOX2
Alternative Names Transcription factor SOX-2, SOX2
Raised in Rabbit
Species Reactivity Human, Mouse
Immunogen Recombinant Human Transcription factor SOX-2 protein (1-317AA)
Immunogen Species Human
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Citations

Progesterone Receptor Membrane Component 1 suppresses the p53 and Wnt/β-catenin pathways to promote human pluripotent stem cell self-renewal. Kim JY.et al,Sci Rep,2018

Application: Western blotting
Sample type: Cells
Review: Western blot analysis showing the expression levels of OCT4, NANOG, SOX2, and PGRMC1 in control or PGRMC1 knockdown hPSCs. β-actin was used as internal protein control and loading control.
PMID: 29445107

Target Data

Function Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).
Involvement in disease Microphthalmia, syndromic, 3 (MCOPS3)
Subcellular Location Nucleus
Database Links

HGNC: 11195

OMIM: 184429

KEGG: hsa:6657

STRING: 9606.ENSP00000323588

UniGene: Hs.518438

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