Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Transcription
Alternative Names
ANOP3; cb236; Delta EF2a; lcc; MCOPS3; MGC148683; MGC2413; RGD1565646; Sex determining region Y box 2; SOX 2; Sox2; SOX2_HUMAN; SRY (sex determining region Y) box 2; SRY box containing gene 2; SRY related HMG box 2; SRY related HMG box gene 2; SRY-box 2; Transcription factor SOX 2; Transcription factor SOX-2; ysb
Species
Homo sapiens (Human)
Expression Region
1-317aa
Target Protein Sequence
MYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHMKEHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSYAHMNGWSNGSYSMMQDQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMNGSPTYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLPGAEVPEPAAPSRLHMSQHYQSGPVPGTAINGTLPLSHM
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
To produce recombinant Human SOX2 protein, a well-established recombinant DNA technology is the key. A DNA template of SOX2 was constructed with N-terminal 6xHis-SUMO tag using the technique. Once the template was made, the recombinant Human SOX2 protein could be produced with it efficiently. CUSABIO has built a strict QC system to ensure quality. The expression region is 1-317aa of the Human SOX2. The purity of this recombinant is 85% determined by SDS-PAGE.
SOX2 is a protein coding gene that encodes Transcription factor SOX-2. According to some studies, SOX2 may have the following features.
The basic function of Sox2 is to stabilize ES cells in a pluripotent state by maintaining the necessary expression levels of Oct3/4. Oct4 and Sox2 bind to the Nanog promoter in live mouse and human embryonic stem cells. Pluripotent cell lines in early mouse development depend on SOX2 function. Constitutive expression of SOX2 inhibits neuronal differentiation and leads to the maintenance of primary characteristics. SOX2 controls tumorigenesis and cancer stem cell function in squamous cell carcinoma. SOX2 is an amplified linear survival oncogene in lung and esophageal squamous cell carcinoma.
