TUBB3 Antibody

Code CSB-PA03874A0Rb
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
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  • Western blot
    All lanes: TUBB3 antibody at 6µg/ml
    Lane 1: A549 whole cell lysate
    Lane 2: A431 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 51, 43 kDa
    Observed band size: 51 kDa

  • Western Blot
    Positive WB detected in: Mouse spleen tissue, Mouse thymus tissue
    All lanes: TUBB3 antibody at 1.5µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 51, 43 kDa
    Observed band size: 51 kDa

  • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA03874A0Rb at dilution of 1:100

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) TUBB3 Polyclonal antibody
Uniprot No. Q13509
Target Names TUBB3
Alternative Names beta 3 tubulin antibody; beta 4 antibody; beta-4 antibody; CDCBM antibody; CDCBM1 antibody; CFEOM3 antibody; CFEOM3A antibody; FEOM3 antibody; M(beta)3 antibody; M(beta)6 antibody; MC1R antibody; Neuron specific beta III Tubulin antibody; Neuron-specific class III beta-tubulin antibody; QccE-11995 antibody; QccE-15186 antibody; TBB3_HUMAN antibody; Tubb 3 antibody; TUBB3 antibody; TUBB4 antibody; Tubulin beta 3 antibody; Tubulin beta 3 chain antibody; Tubulin beta 4 antibody; Tubulin beta III antibody; Tubulin beta-3 chain antibody; Tubulin beta-4 chain antibody; Tubulin beta-III antibody; tuj 1 antibody; tuj1 antibody
Raised in Rabbit
Species Reactivity Human, Mouse
Immunogen Recombinant Human Tubulin beta-3 chain protein (1-210AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

High glucose‐mediated PICALM and mTORC1 modulate processing of amyloid precursor protein via endosomal abnormalities. CW Chae,Br. J. Pharmacol,2020

Applications: Western Blot
Sample type: Cells
Review: The cells were incubated with mithramycin A (25 nM) for 30 min prior to high glucose treatment (25 mM) for 24 h. PICALM, AP2A1, CHC, and β-actin were subjected to western blot. n = 5 from independent experiments. *p < 0.05 vs. control, #p < 0.05 vs. high glucose.
PMID: 32436237

The Networks of Genes Encoding Palmitoylated Proteins in Axonal and Synaptic Compartments Are Affected in PPT1 Overexpressing Neuronal-Like Cells. Pezzini F.et al,Front Mol Neurosci.,2017

Application: Western Blotting Analysis
Antibody dilution factor: 1:1000
Sample type: Cellular pellets
Review: Semi-quantitative WB analysis confirmed a decreased expression of β-III tubulin in SH-p.wtCLN1, following differentiation in RA-NBM medium. GAPDH served as internal standard; a.u. arbitrary units; mean ± SEM of three independent experiments; unpaired t-test; ∗p < 0.05.
PMID: 28878621

Target Data

Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
Gene References into Functions
  1. Results demonstrated that TUBB3 mutations impair the interaction of DCC with polymerized TUBB3 in MTs and inhibit netrin-1-induced neurite outgrowth, branching and attraction. These results suggest a disease mechanism underlying TUBB3 mutation-associated axon guidance defects: mutations in TUBB3 disrupt engagement of netrin/DCC signaling with MT dynamics, resulting in specific defects of netrin-1-mediated axon projection. PMID: 29382549
  2. The remarkable high prevalence of TUBB3 expression in head and neck squamous cell carcinomas emphasizes its putative relevance as a target for future drugs targeting TUBB3 PMID: 28640948
  3. TUBB3 and TUBB4 are necessary for the transport and proper localization of N-cadherin within the plasma membrane. PMID: 28648944
  4. The miR-34a/STMN1/betaIII-tubulin axis maintains the microtubule cytoskeleton in osteosarcoma, and combining miR-34a with microtubule inhibitors can be investigated as a novel therapeutic strategy. PMID: 28275089
  5. Tubulin beta 3 (TUBB3) expression was identified as a useful predictive factor for survival. PMID: 29491095
  6. This is the first study that relates concurrent expression of Tubbeta3, ZEB1, and beta-catenin to abnormal epithelial and myofibroblast differentiation in idiopathic pulmonary fibrosis, providing indirect but robust evidence of miR-200 deregulation and epithelial to mesenchymal transition activation in idiopathic pulmonary fibrosis. PMID: 27586205
  7. The authors found that transient TUBB3 activation, through ABCB1, in response to the stimulation of FOXO3a expression, significantly contributes to the cross-resistance of the paclitaxel-resistant cell population and consequently limits the efficacy of both agents where cancer cells have developed multiple resistance. PMID: 27284014
  8. By linkage and sequencing analyses, we identified the mutation c.784C>T (p.R262C) in TUBB3 gene in six members of a Turkish family with congenital fibrosis of the extraocular muscles. PMID: 27428177
  9. High TUBB3 expression is associated with Melanoma. PMID: 28024114
  10. these results identify a broad spectrum of cancers that can at least sporadically express TUBB3. Testing of TUBB3 in cancer types eligible for taxane-based therapies could be helpful to identify patients who might best benefit from this treatment. PMID: 29022485
  11. TUBB3 overexpression is linked to an aggressive subtype of urinary bladder cancers, which is characterized by increased genetic instability, p53 alterations, and rapid cell proliferation. PMID: 28025079
  12. The central findings of our study are that TSA and VPA affect the expression of neuronal lineage genes in an rMSCs culture. After exposure to TSA or VPA, the expression of early neuronal gene decreases but equally the expression of mature neuron genes increases PMID: 28397704
  13. a positive correlation was observed between Snail presence and TUBB3 upregulation in tested adenocarcinoma cell lines. PMID: 27188792
  14. This elucidates a hitherto unknown role for this microtubule protein and provides insight into correlations between high betaIII-tubulin expression and poor patient outcome in this disease PMID: 27207668
  15. TUBB3 involvement in breast cancer cells apoptosis PMID: 28075472
  16. The expressions of TYMS, TUBB3 and STMN1 were significantly associated with the clinicopathological characteristics of age, gender and family history of gastric cancer, but not with differentiation, growth patterns, metastasis and TNM staging in patients with gastric cancer. PMID: 28056823
  17. It findings reveal that expression of relatively low levels of TUBB3 in mitotic cells can be detrimental for their cytokinesis and underscore the importance of CIT-K in counteracting this event. PMID: 26586574
  18. Epitaxial growth of alpha1A/betaIII microtubules from heterogeneous brain seeds is inefficient but can be fully rescued by incorporating as little as 5% of brain tubulin into the homogeneous alpha1A/betaIII lattice. PMID: 27129203
  19. TUBB3 mutations cause both Congenital Fibrosis of the Extraocular Muscles type 3 and malformations of cortical development. PMID: 26639658
  20. The decreased expression of TUBB3 could be a significant marker for predicting unfavourable prognosis in patients with cutaneous malignant melanoma. PMID: 26426765
  21. High TUBB3 mRNA expression was associated with breast cancer. PMID: 26406408
  22. mutations proximal to the TUBB3 kinesin-binding site alter polymerization dynamics. PMID: 27046833
  23. Functional characterization of MC1R-TUBB3 intergenic splice variants of the human melanocortin 1 receptor has been undertaken in response to ultraviolet irradiation. PMID: 26657157
  24. TUBB3, TOP2A, CYP19A1 and CYP2D6 gene expression, but not protein expression, was associated with patient survival in breast cancer . PMID: 26252353
  25. In advanced cervical squamous cell cancer, TUBB3 was highly expressed in 20.%% of cases. It had no impact on survival in patients treated with antimicrotubule agents. PMID: 26165688
  26. Findings suggest that overexpression of Class III beta-tubulin, Sox2, and nuclear Survivin might be predictive of taxane resistance and poor progression-free survival in patients with stage III ovarian epithelial cancer. PMID: 26198101
  27. High level of stathmin exhibited poor response to chemotherapy . Similar effects were not observed for b-III-tubulin. PMID: 25894372
  28. Results show an overexpression of TUBB3 in breast cancer brain metastases and its expression was significantly associated with distant metastases. These findings suggest a novel role of TUBB3 in regulation of metastases and integrin-Src signaling. PMID: 25724666
  29. Depletion of betaIII-tubulin from MCF7 breast cancer cells increased mitotic arrest by ixabepilon. Increased betaIII-tubulin may be an important contributor to the development of resistance to ixabepilone. PMID: 26416565
  30. Data suggest that silencing of beta III-tubulin expression may be a potential therapeutic strategy to increase the long-term survival of pancreatic cancer patients. PMID: 25544769
  31. blocking of the beta-III tubulin expression in colorectal cancer cells does not affect their viability, but reduces the cell adhesion to the extracellular matrix PMID: 26591579
  32. Results show that cabazitaxel resistance mechanisms involve alterations in microtubule dynamicity, as manifested by higher expression of TUBB3 and decreased BRCA1. PMID: 25416788
  33. These results therefore suggested that certain TUBB3 mutations may predominantly be associated with axonal peripheral neuropathy. PMID: 25482575
  34. This review discusses the confirmation that Gli1 overexpression is associated with decreased response to tumor chemotherapy, it was possible to sensitize cells to epothilones after addition a suitable inhibitor. PMID: 25661915
  35. High TUBB3 expression in NSCLC appeared to correlate with lower PTX sensitivity PMID: 25964544
  36. This study revealed that TUBB3 is a moderately sensitive and highly specific tissue marker for discriminating peripheral intrahepatic cholangiocarcinomas from other primary liver tumours. PMID: 25039376
  37. First report of an autosomal-dominant inheritance of the TUBB3 c.1228G>A mutation in a family provides new insights into the spectrum and variability of endocrine phenotypes associated with the TUBB3 E410K syndrome. PMID: 25559402
  38. ERCC1 mRNA levels were higher in metastatic adenocarcinoma NSCLC; TUBB3 mRNA levels were significantly higher in poorly differentiated tumors and in advanced stage NSCLC, which indicates the poor prognosis. PMID: 24762590
  39. Findings revealed how betaIII-tubulin influences tumor growth in NSCLC, defining new biologic functions and mechanism of action of betaIII-tubulin in tumorigenesis. PMID: 25414139
  40. betaIII-tubulin down-regulated significantly sensitized NSCLC A549/Taxol cells to Paclitaxel PMID: 25130963
  41. evidence for a direct linkage between HIF-2alpha and increased expression of betaIII-t by hypoxia PMID: 25244496
  42. Our data support the use of TUBB3 staining in differentiating follicular lymphoma, including BCL2-negative cases, from follicular hyperplasia PMID: 24232867
  43. Data indicate that ABCB1 protein, beta tubulin I and III (betaI, and betaIII tubulin) might contribute to the multidrug resistance (MDR) of MCF7/DOC and be potential therapeutic targets for overcoming MDR of breast cancer. PMID: 24894670
  44. The sensitivity of gastric cancer patients to paclitaxel treatment was inversely correlated with the mRNA and protein expression of class III beta-tubulin. PMID: 24722794
  45. The expression level of TUBB3 may be a useful biomarker to predict the clinical outcomes of the taxane/vinorebine-based chemotherapy in patients with NSCLC. PMID: 24705847
  46. Patients with exotropia, vertical gaze limitation, and ptosis do not have classic Moebius syndrome and may have TUBB3 mutations associated with CFEOM3. PMID: 24612975
  47. TUBB3 was a strong predictive marker in recurrent and metastatic gastric cancer patients receiving taxane-based first-line palliative chemotherapy. PMID: 24053422
  48. Sox9 allows the survival of ovarian cells upon hypoxic condition, through the activation of betaIII-tubulin expression. PMID: 24661907
  49. High tubulin beta 3 expression is associated with malignant pleural mesothelioma. PMID: 23810210
  50. Higher response rates were observed among betaIII-tubulin-positive patients. PMID: 23853246
  51. Mutation of EGFR E21 is negatively correlated with TUBB3 gene expression in non-small cell lung cancer. PMID: 24113009
  52. TUBB3 expression may be a predictive marker for chemoresistance to docetaxel in NSCLC with postoperative recurrent disease PMID: 22358390
  53. Results indicate that Yg-3-46a has the potential to evade drug resistance mediated by P-gp and beta-III tubulin overexpression. PMID: 23941826
  54. results suggest that in aggressive breast cancers, as modeled by MDA-MB-231 cells, beta-tubulin class III is a biomarker for cell survival mediated through ZEB1-induced tumor progression pathways. PMID: 23869586
  55. Tubulin-beta-III overexpression in Uterine serous carcinoma discriminates poor prognosis, serves as a marker for sensitivity to epothilones, and may contribute to paclitaxel resistance PMID: 23585021
  56. human melanocytes express TUBB3 and that this protein should be recognized as a wider marker for multiple neural crest-derived cells. PMID: 23817083
  57. Gastric cancer tumors with low TUBB3 expression demonstrate improved response to cytotoxic therapy. PMID: 23532817
  58. High expression levels of tubulin beta 3 class III is associated with response to therapy in estrogen receptor-negative breast cancer. PMID: 23218766
  59. GLI3, fascin and TUBB3 are the most sensitive markers in Hodgkin lymphoma and anaplastic large cell lymphoma. PMID: 23454725
  60. low levels of TUBB3 in serum could predict better response and survival for advanced gastric cancer patients receiving paclitaxel plus capecitabine. PMID: 22766748
  61. A novel syndrome caused by E410K amino acid substitution in TUBB3 is linked to Kallmann syndrome, cyclic vomiting and Moebius syndrome. PMID: 23378218
  62. In postoperative NSCLC patients who are receiving adjuvant chemotherapy, patients with high expression of beta-tubulin 3 tend to be resistant to taxane drugs. PMID: 20868593
  63. negative REST and positive TUBB3 protein expression was detected as independent prognostic factors. PMID: 22684772
  64. in colorectal cancer patients, there was a link between poor survival, the expression of TUBB3/TUBB6, and androgen receptor only in females PMID: 22438565
  65. High beta III-tubulin expression is associated with drug resistance to docetaxel in patients with advanced breast cancer. PMID: 22502701
  66. Overexpression of betaIII-tubulin is associated with drug resistance to docetaxel-based chemotherapy in advanced gastric cancer. PMID: 22740207
  67. Higher levels of TUBB3 were detected in uterine carcinosarcoma (CS) cell lines and fresh frozen tissues when compared to ovarian CS and were associated with sensitivity to patupilone. PMID: 22209775
  68. TUBB3 expression occurs in a cell cycle-dependent manner. PMID: 22159867
  69. Data suggest that the increased betaII- and betaIII-tubulin contributed significantly to the resistance phenotype. PMID: 22180309
  70. The expression level of BRCA1 and class IIIbeta-tubulin in tumor tissue is probably a predictor for the efficacy of chemotherapy in non-small cell lung cancer patients. PMID: 21781528
  71. Class IIIbeta-tubulin behaves as a gateway for prosurvival signals, such as those of proto-oncogene protein PIM1 in ovarian cancer cells, which are capable of moving into the cytoskeleton under hypoxic conditions. PMID: 21520077
  72. betaIII-Tubulin is required for interphase microtubule dynamics in untransformed human mammary epithelial cells PMID: 21820201
  73. The expression of III beta-tubulin and MDR1 may play an important role in the development and progression of human non-small cell lung cancer. PMID: 21163067
  74. HER2 and TUBB3 status might be a good biomarker for determining the most appropriate therapeutic modality in extramammary Paget disease. PMID: 20534991
  75. class III beta-tubulin expression in prechemotherapy effusions is associated with poor chemoresponse and shorter survival in serous ovarian carcinoma. PMID: 21315408
  76. In Chinese advanced gastric cancer, Thymidine Phosphorylase positive & beta-tubulin III negative might predict response and prognosis to capecitabine plus paclitaxel chemotherapy. PMID: 21586171
  77. High TUBB3 is associated with thymic epithelial tumors. PMID: 21289518
  78. The human melanocortin 1 receptor gene, has a highly complex and inefficient poly(A) site which is instrumental in allowing intergenic splicing between this locus and its immediate downstream neighbour tubulin-beta-III (TUBB3). PMID: 21071418
  79. Mutations in the neuronal beta-tubulin subunit TUBB3 result in malformation of cortical development and neuronal migration defects. PMID: 20829227
  80. Findings suggest a role for betaIII-tubulin as candidate theranostic biomarker to predict the response to docetaxel-based chemotherapy as well as to target for treatment of docetaxel-resistant CRPC. PMID: 21045157
  81. congenital fibrosis of extraocular muscle type 3 caused by TUBB3 R262C and D417N amino acid substitutions features abnormalities: subarachnoid CN3 hypoplasia, occasional abducens nerve hypoplasia, and subclinical optic nerve hypoplasia PMID: 20393110
  82. HuR gene silencing revealed that TUBB3 translation is HuR dependent in hypoglycemia because HuR silencing inhibited the entry of TUBB3 mRNA into cytoskeletal and free polysomes. PMID: 20587520
  83. betaIII tubulin expression is emerging as a valuable biomarker for taxane resistance in advanced disease, as well as offering prognostic information for outcomes among patients with earlier stage disease. PMID: 20403547
  84. A variety of neoplastic and non-neoplastic lymphoproliferative disorders exhibited characteristic TUBB3 expression patterns. PMID: 20220512
  85. Over-expression of class III beta-tubulin is associated with resected non-small cell lung cancer. PMID: 20087230
  86. This paper reviews the evidence base for betaIII tubulin expression as a prognostic and predictive biomarker inN on-small cell lung cancer PMID: 19828208
  87. determination of the expression of excision repair cross-complementation group 1 and class IIIbeta tubulin is useful to predict the effects of platinum-based anticancer drugs. PMID: 20021611
  88. Work to define the TUBB3 syndromes establishes the requirement for a neuronal beta-tubulin isotype in axon guidance and normal brain development. PMID: 20074521
  89. Patients with low beta-tubulin III levels had better response in the paclitaxel/carboplatin PMID: 12789263
  90. Human brain and testis specific betaIII-tubulin diminishes microtubule assembly, and is able to confer weak resistance to paclitaxel when expressed at moderate levels in mammalian cells. PMID: 12905530
  91. class III beta-tubulin has a role in paclitaxel resistance in ovarian cancer PMID: 15671559
  92. high level of expression of class III beta-tubulin in tumor cells is associated with resistance to vinorelbine and a poor prognosis in patients with NSCLC receiving vinorelbine-based chemotherapy PMID: 16061864
  93. useful to identify poor prognosis ovarian cancer patients candidates to more aggressive and/or targeted therapy. PMID: 16675570
  94. Deleterious post-translational modifications of beta 3 tubulin accumulate in a pathological protein fraction in Alzheimer dissease. PMID: 16816122
  95. bTubIII has a role in progression of non-small cell lung cancer PMID: 17289895
  96. Data suggest that aberrant expression and interactions of betaIII-tubulin and gamma-tubulin may be linked to malignant changes in glial cells. PMID: 17406983
  97. study concludes that class III beta-tubulin is expressed in some cases of major primary brain tumour types except pilocytic astrocytoma; extent of class III beta-tubulin expression is variable PMID: 17543088
  98. TUBB3 is expressed in most pancreatic ductal adenocarcinomas. Up-regulation of TUBB3 in PanIN lesions suggests that microtubule dysfunction is an early feature of this disease. PMID: 17714470
  99. Methylation analysis of 3' enhancer showed that it was free of methylation in 70% cells in A2780, while in less than 16% in both TC1 and HeLa cells, thereby suggesting that TUBB3 increase upon hypoxia is abolished through methylation of the 3' enhancer. PMID: 18178340
  100. In the midgestational human brain, betaIII-tubulin is not neuron specific because it is constitutively expressed in GFAP+/nestin+ presumptive fetal astrocytes PMID: 18379434

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Involvement in disease Fibrosis of extraocular muscles, congenital, 3A (CFEOM3A); Cortical dysplasia, complex, with other brain malformations 1 (CDCBM1)
Subcellular Location Cytoplasm, cytoskeleton
Protein Families Tubulin family
Tissue Specificity Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues.
Database Links

HGNC: 20772

OMIM: 600638

KEGG: hsa:10381

STRING: 9606.ENSP00000320295

UniGene: Hs.511743

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