UBE2G1 Antibody

Datasheet

Code	CSB-PA025453LA01HU
Size	US$166
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Image	IHC image of CSB-PA025453LA01HU diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA025453LA01HU diluted at 1:500 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA025453LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) UBE2G1 Polyclonal antibody

Uniprot No.

P62253

Target Names

UBE2G1

Alternative Names

E217K antibody; UB2G1_HUMAN antibody; UBC 7 antibody; UBC7 antibody; UBC7 homolog yeast antibody; UBE 2G antibody; UBE2G antibody; Ube2g1 antibody; Ubiquitin carrier protein G1 antibody; Ubiquitin conjugating enzyme E2 G1 antibody; Ubiquitin conjugating enzyme E2G 1 (homologous to C. elegans UBC7) antibody; Ubiquitin conjugating enzyme E2G 1 (UBC7 homolog C. elegans) antibody; Ubiquitin conjugating enzyme E2G 1 (UBC7 homolog yeast) antibody; Ubiquitin conjugating enzyme E2G 1 antibody; Ubiquitin protein ligase G1 antibody; Ubiquitin-conjugating enzyme E2 G1 antibody; Ubiquitin-protein ligase G1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Ubiquitin-conjugating enzyme E2 G1 protein (2-170AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The UBE2G1 Antibody (Product code: CSB-PA025453LA01HU) is Non-conjugated. For UBE2G1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA025453LB01HU	UBE2G1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA025453LC01HU	UBE2G1 Antibody, FITC conjugated
Biotin	CSB-PA025453LD01HU	UBE2G1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4.

Gene References into Functions

ubiquitin binding by the acidic loops of Ube2g1 and Ube2r1 enzymes distinguishes their Lys-48-ubiquitylation activities PMID: 25471371
study reports that UBCH8 and UBE2G1 and UBE2G2 cooperate with CRL4Cdt2 in promoting the polyubiquitylation and subsequent degradation of p21 and Cdt1, respectively PMID: 21628527
In endoplasmic reticulum, involved in the degradation of type 2 iodothyronine selenodeiodinase PMID: 12933904

Protein Families

Ubiquitin-conjugating enzyme family

Tissue Specificity

Widely expressed, mainly in skeletal muscle.

Database Links

HGNC: 12482

OMIM: 601569

KEGG: hsa:7326

STRING: 9606.ENSP00000380178

UniGene: Hs.741319

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Protein processing in endoplasmic reticulum Ubiquitin mediated proteolysis

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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