WDR5 Antibody

Code CSB-PA026038ESR2HU
Size US$166
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  • Western blot
    All lanes: WDR5 antibody at 2µg/ml + A431 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 37 kDa
    Observed band size: 37 kDa

  • Immunohistochemistry of paraffin-embedded human breast tissue using CSB-PA026038ESR2HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA026038ESR2HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) WDR5 Polyclonal antibody
Uniprot No.
Target Names
WDR5
Alternative Names
2410008O07Rik antibody; AA408785 antibody; AA960360 antibody; BIG 3 antibody; Big antibody; BIG3 antibody; BMP2 induced 3 kb gene protein antibody; BMP2 induced gene; 3-KB antibody; BMP2-induced 3-kb gene protein antibody; MGC114572 antibody; OTTHUMP00000162494 antibody; SWD 3 antibody; SWD3 antibody; SWD3 Set1c WD40 repeat protein homolog antibody; WD repeat containing protein 5 antibody; WD repeat domain 5 antibody; WD repeat protein 5 antibody; WD repeat-containing protein 5 antibody; WD repeat-containing protein BIG-3 antibody; WDR 5 antibody; Wdr5 antibody; WDR5_HUMAN antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human WD repeat-containing protein 5 protein (1-334AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:200-1:1000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation. In association with RBBP5 and ASH2L, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B.
Gene References into Functions
  1. WDR5 was identified as a substrate of SETD6 and it was revealed that the methylation of specific lysines (K207/K325) of WDR5 was critical for maintaining global histone H3K4me3 levels and promoting breast cancer cell proliferation and migration PMID: 30226578
  2. WDR5 is aberrantly overexpressed in head neck squamouos cell carcinoma and associates with aggressiveness and unfavorable prognosis, thus representing a novel diagnostic and prognostic biomarker for the cancer. PMID: 29569374
  3. This is the first demonstration of the mechanism of E2/ERalpha signalling activation via the BIG3-PKA-PP1Calpha tri-complex in breast cancer cells. PMID: 28555617
  4. Data found that the expression of WDR5 increased in a majority of the human gastric cancer tissues. Its knockdown significantly inhibited the ability of cell proliferation, reversed cell cycle arrest as well as cell's tumorigenicity. Data reveal that WDR5 may have oncogenic effect and WDR5-mediated H3K4 methylation plays an important role in gastric cancer. PMID: 29125890
  5. findings reveal a nongenomic function for WDR5 in regulating H3K4 methylation induced by 3D environments, physical properties of the nucleus, cell polarity, and cell migratory capacity. PMID: 29987046
  6. HCMV infection increases protein levels of WD repeat-containing protein 5 (WDR5) during infection, overexpression of WDR5 enhances viral replication, and knockdown of WDR5 dramatically attenuates viral replication. The results indicate that WDR5 promotes the nuclear egress of viral capsids, the depletion of WDR5 resulting in a significant decrease in production of infectious virions. PMID: 29437978
  7. our findings indicate that HOXD-AS1 promotes proliferation, castration resistance, and chemo-resistance in prostate cancer by recruiting WDR5. PMID: 28487115
  8. Functional analysis implicates TNRC6A, NAT10, MED14, and WDR5 in RNA-mediated transcriptional activation. PMID: 28813667
  9. The decrease in WDR5 expression was particularly evident in HD-hiPSCs compared to hESCs and control-hiPSCs from healthy subjects PMID: 28476540
  10. we demonstrate for the first time that targeted expression of miR-31-5p using a nonviral minicircle vector can serve as a novel approach for tumor miRNA therapy. Moreover, WDR5 may be a promising therapeutic target for NPC treatment. PMID: 28042945
  11. WDR5 may have oncogenic effect and WDR5-mediated H3K4 methylation plays an important role in leukemogenesis PMID: 27192115
  12. WDR5 functions to sustain proper execution of DNA replication in pancreatic ductal adenocarcinoma cells, as previously suggested by replication stress studies involving MLL1, and c-Myc, also found to interact with WDR5. PMID: 27320920
  13. This report documents a novel lncRNA, GClnc1, which may act as a scaffold to recruit the WDR5 and KAT2A complex and modify the transcription of target genes. This study reveals that GClnc1 is an oncogenic lncRNA in human gastric cancer. PMID: 27147598
  14. different cancer mutations in MLL1 lead to a loss or increase in activity, illustrating the complex and tumor-specific role of MLL1 in carcinogenesis. PMID: 28182322
  15. WDR5 shows a direct binding to the ZNF407 promoter. PMID: 28300833
  16. Taken together, these data suggest that WDR5 is directly involved in p53 signaling pathway. Our studies provide a new insight into WDR5 functions in A549 cells. PMID: 28412363
  17. Targeted Disruption of the Interaction between WD-40 Repeat Protein 5 (WDR5) and Mixed Lineage Leukemia (MLL)/SET1 Family Proteins Specifically Inhibits MLL1 and SETd1A Methyltransferase Complexes. PMID: 27563068
  18. WDR5 over-expression is associated with poor breast cancer clinical outcome. PMID: 26355959
  19. Results identify WDR5 as a key cofactor for N-Myc-regulated transcriptional activation and tumorigenesis and as a novel therapeutic target for MYCN-amplified neuroblastomas. PMID: 26471359
  20. BIG3 may block the KPNAs (KPNA1, KPNA5, and KPNA6) binding region(s) of PHB2. PMID: 26052702
  21. solution structures of the MLL3 core complex assembled with and without WDR5 by small angle x-ray scattering show similar overall topologies PMID: 26324722
  22. Target gene recognition by MYC depends on its interaction with WDR5. This interaction could create an avidity-based chromatin recognition mechanism allowing MYC to select its target genes in response to both genetic and epigenetic determinants. Review. PMID: 26383167
  23. we have discovered that WDR5 plays an important role in bladder cancer suggesting that WDR5 is a potential biomarker and a promising target in the treatment of bladder cancer. PMID: 25656485
  24. Wdr5-MLL interaction in C/EBPA N-terminal leukemia is a promising pharmacological target. PMID: 26167872
  25. findings prove that the TSP-1/CD47/SIRP-alpha signal axis is important to the evolution of tumor cells in the microenvironment of immunotherapy and identify thrombospondin-1 as a key signal PMID: 25666610
  26. results identify WDR5 as a critical epigenomic integrator of histone phosphorylation and methylation and as a major driver of androgen-dependent prostate cancer cell proliferation PMID: 24793694
  27. BIG3(WRD5)-PHB2 interaction is critical for the tamoxifen resistance of breast cancer cells; its targeting reverses the resistance. PMID: 24051437
  28. Data indicate that MLL1 methylates Ash2L in the absence of histone H3, but only when assembled within a complex with WDR5 and RbBP5. PMID: 24235145
  29. data are consistent with a model in which WDR5 binds the gamma-globin promoter in a PRMT5-dependent manner. PMID: 22689669
  30. A small molecule antagonist binds to WDR5 in the peptide-binding pocket which inhibits the catalytic activity of mixed-lineage leukemia protein. PMID: 22989411
  31. we defined a role for Trithorax proteins WDR5 and MLL2 in activating the differentiation gene program in epidermal progenitor cells PMID: 22829784
  32. WDR5-Win motif interaction is important for the assembly of the MLL1 core complex in vivo. PMID: 22665483
  33. findings show that WDR5 is a direct target of SRY; the interaction of WDR5 and SRY activates Sox9 expression; results suggest that, in conjunction with SRY, WDR5 plays an important role in sex determination PMID: 22523547
  34. WDR5 as a critical substrate of CUL4B in regulating neuronal gene expression. PMID: 21816345
  35. crystal structure of WDR5 in ternary complex with RbBP5 and MLL1 PMID: 21220120
  36. the change of folding free energy by mutations mainly corresponds to the deletion of hydrogen bonds. PMID: 20939513
  37. NSL is composed of nine subunits. Two of its subunits, WD repeat domain 5 (WDR5) and host cell factor 1 (HCF1), are shared with members of the MLL/SET family of histone H3 lysine 4 (H3K4) methyltransferase complexes. PMID: 20018852
  38. Results show that Depletion of CHD8 enhances HOXA2 expression and a loss of the WDR5/Ash2L/RbBP5 subcomplex. PMID: 20085832
  39. WDR5 is essential in assembling a virus-induced VISA-associated complex and plays an important role in virus-triggered induction of type I interferons. PMID: 20080758
  40. The WDR5 is required for binding of the methyltransferase complex to the K4-dimethylated H3 tail as well as for global H3 K4 trimethylation and HOX gene activation in human cells. PMID: 15960974
  41. Wdr5 has a funcitonal role in endocondral bone formation PMID: 16340128
  42. the high-resolution X-ray structures of WDR5 in the unliganded form and complexed with histone H3 peptides having unmodified and mono-, di- and trimethylated K4 PMID: 16829959
  43. the crystal structure of WDR5 bound to a histone H3 PMID: 16829960
  44. WDR5 mediates interactions of the MLL1 catalytic unit both with the common structural platform and with the histone substrate. PMID: 16878130
  45. involvement of WDR5 in binding and presenting histone H3K4 for further methylation PMID: 16946699
  46. WDR5's recognition of arginine 3765 of MLL1 is essential for the assembly and enzymatic activity of the MLL1 core complex in vitro PMID: 18829457
  47. WDR5 recognizes Arg-3765 of MLL1, which is essential for the assembly and enzymatic activity of the MLL1 core complex PMID: 18829459
  48. analysis of interactions between WDR5, the catalytic subunit, MLL, and the substrate, histone H3, of the MLL complex PMID: 18840606
  49. The identified components revealed factors involved in histone methylation and cell cycle control and include Ash2L, RbBP5, WDR5, HCF-1, DBC-1, and EMSY. PMID: 19131338
  50. Activation of an estrogen/estrogen receptor signaling by BIG3 through its inhibitory effect on nuclear transport of PHB2/REA in breast cancer is reported. PMID: 19496786

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Subcellular Location
Nucleus.
Protein Families
WD repeat WDR5/wds family
Database Links

HGNC: 12757

OMIM: 609012

KEGG: hsa:11091

STRING: 9606.ENSP00000351446

UniGene: Hs.397638

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