ZRANB1 Antibody

Datasheet

Code	CSB-PA883378LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate, HL-60 whole cell lysate All lanes: ZRANB1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81 kDa Observed band size: 81 kDa IHC image of CSB-PA883378LA01HU diluted at 1:400 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA883378LA01HU diluted at 1:400 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ZRANB1 Polyclonal antibody

Uniprot No.

Q9UGI0

Target Names

ZRANB1

Alternative Names

DKFZp762P2216 antibody; hTrabid antibody; Trabid antibody; TRAF-binding protein domain antibody; Ubiquitin thioesterase ZRANB1 antibody; Zinc finger Ran-binding domain-containing protein 1 antibody; ZRAN1_HUMAN antibody; ZRANB1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Ubiquitin thioesterase ZRANB1 protein (111-397AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ZRANB1 Antibody (Product code: CSB-PA883378LA01HU) is Non-conjugated. For ZRANB1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA883378LB01HU	ZRANB1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA883378LC01HU	ZRANB1 Antibody, FITC conjugated
Biotin	CSB-PA883378LD01HU	ZRANB1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration. May also modulate TNF-alpha signaling.

Gene References into Functions

Data show that the ankyrin-repeat ubiquitin-binding domain is crucial for TRABID efficiency and linkage specificity. PMID: 22157957

Subcellular Location

Cytoplasm. Nucleus. Note=Enriched in punctate localization in the cytoplasm.

Protein Families

Peptidase C64 family

Tissue Specificity

Widely expressed.

Database Links

HGNC: 18224

OMIM: 611749

KEGG: hsa:54764

STRING: 9606.ENSP00000352676

UniGene: Hs.595158

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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