Recombinant Bovine Angiopoietin-related protein 4 (ANGPTL4)

Code CSB-YP645629BO
MSDS
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Source Yeast
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Code CSB-EP645629BO
MSDS
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Source E.coli
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Code CSB-EP645629BO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP645629BO
MSDS
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Source Baculovirus
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Code CSB-MP645629BO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ANGPTL4
Uniprot No.
Alternative Names
ANGPTL4Angiopoietin-related protein 4; Angiopoietin-like protein 4) [Cleaved into: ANGPTL4 N-terminal chain; ANGPTL4 C-terminal chain]
Species
Bos taurus (Bovine)
Expression Region
24-410
Target Protein Sequence
QGRPEPP ETPRFASWDE VNVLAHGLLQ LGHGLREHVE RTRGQLGELE RRLGACGAAC KDPEGSAAPP RAQANLVNPG GGDASPETLR SLKTQLEAQN SRIQQLFQKV AQQQRHLEKQ QLRIQNLQSQ MDHLAPRHLG HEMAKPARRK RLPKMAQLAG PAHNISRLHR LPRDCQELFE EGERESGLFQ IQPQGSPPFL VNCKMTSDGG WTVIQRRQDG SVDFNQPWEA YKDGFGDPQG EFWLGLEKVH HILGDRGSRL AVQLQDWEGN AESLQFPIHL GGEDTAYSLQ LTPPVASKLG ATTFSPSGLS LPFSTWDQDH DLRGDKNCAR SLSGGWWFGT CSHSNLNGQY FHSIPRQRQQ RKKGIFWKTW RGRYYPLQAT TILVQPTAAS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Mediates inactivation of the lipoprotein lipase LPL, and thereby plays a role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism. May also play a role in regulating glucose homeostasis and insulin sensitivity. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. Upon heterologous expression, inhibits the adhesion of endothelial cell to the extracellular matrix (ECM), and inhibits the reorganization of the actin cytoskeleton, formation of actin stress fibers and focal adhesions in endothelial cells that have adhered to ANGPTL4-containing ECM (in vitro). Depending on context, may modulate tumor-related angiogenesis.; Mediates inactivation of the lipoprotein lipase LPL, and thereby plays an important role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism. Has higher activity in LPL inactivation than the uncleaved protein.
Gene References into Functions
  1. increase in angiopoietin-like protein 4 messenger RNA across multiple models of altered energy balance identifies it as an adipokine that is uniquely responsive to changes in energy balance in the lactating dairy cow PMID: 22683322
  2. These findings indicate that liver and adipose tissue are key sources of ANGPTL4 in cattle; the protein was also highly abundant in ruminal epithelium, making it possible that commensal microbes may influence ANGPTL4 synthesis and secretion. PMID: 19783696
Subcellular Location
Secreted. Secreted, extracellular space, extracellular matrix.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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