Recombinant Bovine DNA damage-binding protein 2(DDB2)

Product Type Recombinant Protein
Uniprot No. Q0VBY8
Abbreviation DDB2
Species Bos taurus (Bovine)
Gene Names DDB2
Protein Names Recommended name: DNA damage-binding protein 2 Alternative name(s): Damage-specific DNA-binding protein 2
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Target Details This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. This subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities.
HGNC 2718
MGI 1355314
Function Required for DNA repair. Binds to DDB1 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as the substrate recognition module for the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB1-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB1-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB1-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER (By similarity).
Subcellular Location Nucleus
Protein Families WD repeat DDB2/WDR76 family
Database Links

KEGG: bta:519357

STRING: 9913.ENSBTAP00000027965

UniGene: Bt.33161

Pathway p53 signaling pathway
DNA repair pathway
Ubiquitin mediated proteolysis

Product Details for Different Expression Systems

Product Name Recombinant Bovine DNA damage-binding protein 2(DDB2),Yeast
Code CSB-YP604366BO
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MAPRKRPENQ KTPEVVVRPK SKRNRSPREL EPEAKKLCVK GPGSSRRFDS GLWAGLASLR VPPLCSSIVR ALHQHKLGTA AWPSLQQGLQ QSFLNSLASY RIFQKAAPFD RRATSLAWHP THPSTLAVGS KGGDILLWNF GIKDKPTFIK GIGAGGSITG MKFNPLNTNQ FFTSSMEGTT RLQDFKGNTL RVFASSDTCN VWFCSLDVSV KSRVVVTGDN VGHVILLNMD GRELWNLRMH KKKVTHVALN PCCDWLLATA SVDQTVKIWD LRQVRGKSSF LHSLPHRHPV NAAHFSPDGA QLLTTDQKSE IRVYSACQWD CPPSLIPHPH RHFQHLTPIK ASWHPRYNLI VVGRYPDPNF KSCSPHELRT IDVFDGSSGK IMYQLYDPES SGIMSLNEFN PMGDTLASVM GYHILVWSPE DAGTQK
Expression Region 1-426
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Bovine DNA damage-binding protein 2(DDB2),E.coli
Code CSB-EP604366BO
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MAPRKRPENQ KTPEVVVRPK SKRNRSPREL EPEAKKLCVK GPGSSRRFDS GLWAGLASLR VPPLCSSIVR ALHQHKLGTA AWPSLQQGLQ QSFLNSLASY RIFQKAAPFD RRATSLAWHP THPSTLAVGS KGGDILLWNF GIKDKPTFIK GIGAGGSITG MKFNPLNTNQ FFTSSMEGTT RLQDFKGNTL RVFASSDTCN VWFCSLDVSV KSRVVVTGDN VGHVILLNMD GRELWNLRMH KKKVTHVALN PCCDWLLATA SVDQTVKIWD LRQVRGKSSF LHSLPHRHPV NAAHFSPDGA QLLTTDQKSE IRVYSACQWD CPPSLIPHPH RHFQHLTPIK ASWHPRYNLI VVGRYPDPNF KSCSPHELRT IDVFDGSSGK IMYQLYDPES SGIMSLNEFN PMGDTLASVM GYHILVWSPE DAGTQK
Expression Region 1-426
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Bovine DNA damage-binding protein 2(DDB2),Baculovirus
Code CSB-BP604366BO
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MAPRKRPENQ KTPEVVVRPK SKRNRSPREL EPEAKKLCVK GPGSSRRFDS GLWAGLASLR VPPLCSSIVR ALHQHKLGTA AWPSLQQGLQ QSFLNSLASY RIFQKAAPFD RRATSLAWHP THPSTLAVGS KGGDILLWNF GIKDKPTFIK GIGAGGSITG MKFNPLNTNQ FFTSSMEGTT RLQDFKGNTL RVFASSDTCN VWFCSLDVSV KSRVVVTGDN VGHVILLNMD GRELWNLRMH KKKVTHVALN PCCDWLLATA SVDQTVKIWD LRQVRGKSSF LHSLPHRHPV NAAHFSPDGA QLLTTDQKSE IRVYSACQWD CPPSLIPHPH RHFQHLTPIK ASWHPRYNLI VVGRYPDPNF KSCSPHELRT IDVFDGSSGK IMYQLYDPES SGIMSLNEFN PMGDTLASVM GYHILVWSPE DAGTQK
Expression Region 1-426
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Bovine DNA damage-binding protein 2(DDB2),Mammalian cell
Code CSB-MP604366BO
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MAPRKRPENQ KTPEVVVRPK SKRNRSPREL EPEAKKLCVK GPGSSRRFDS GLWAGLASLR VPPLCSSIVR ALHQHKLGTA AWPSLQQGLQ QSFLNSLASY RIFQKAAPFD RRATSLAWHP THPSTLAVGS KGGDILLWNF GIKDKPTFIK GIGAGGSITG MKFNPLNTNQ FFTSSMEGTT RLQDFKGNTL RVFASSDTCN VWFCSLDVSV KSRVVVTGDN VGHVILLNMD GRELWNLRMH KKKVTHVALN PCCDWLLATA SVDQTVKIWD LRQVRGKSSF LHSLPHRHPV NAAHFSPDGA QLLTTDQKSE IRVYSACQWD CPPSLIPHPH RHFQHLTPIK ASWHPRYNLI VVGRYPDPNF KSCSPHELRT IDVFDGSSGK IMYQLYDPES SGIMSLNEFN PMGDTLASVM GYHILVWSPE DAGTQK
Expression Region 1-426
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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