Recombinant Drosophila melanogaster Transcription factor E2f (E2f), partial

Code CSB-YP630636DLU
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Source Yeast
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Code CSB-EP630636DLU
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Source E.coli
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Code CSB-EP630636DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP630636DLU
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Source Baculovirus
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Code CSB-MP630636DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
E2f1
Uniprot No.
Alternative Names
E2f1; E2f; CG6376; Transcription factor E2f1; dE2F
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional activator that binds to E2f sites. Required for wild-type growth in mitotic and polytene tissues, Contributes to the expression of replication genes at the G1-S transition and Cyclin E. Activates cell proliferation in wing imaginal disk, which requires expression of vg.
Gene References into Functions
  1. dE2F1b is a novel member of the E2F family, revealing a previously unappreciated complexity in the Drosophila RB/E2F network. PMID: 29420631
  2. specific alternate transcripts of activator E2F, dE2F1, may have a dual function on cell cycle progression and cannot simply be viewed as a pro-proliferative transcription factor PMID: 26859702
  3. These findings identify a key function of E2F in skeletal muscle required for animal viability, and illustrate how the cell cycle regulator is repurposed in post-mitotic cells. PMID: 26823289
  4. also demonstrated that an optimum level of dLin52 is needed for dE2F1/2 activity on the hid promoter PMID: 24863159
  5. Loss of dE2F compromises mitochondrial function. PMID: 24286825
  6. Inappropriate accumulation of E2f1 protein during S phase triggers the elimination of potentially hyperplastic cells via apoptosis in order to ensure normal development of rapidly proliferating tissues. PMID: 22916021
  7. endocycles of Drosophila are driven by a molecular oscillator in which the E2F1 transcription factor promotes CycE expression and S-phase initiation, S-phase then activates the CRL4(CDT2) ubiquitin ligase, and this in turn mediates the destruction of E2F1 PMID: 22037307
  8. coexpression of miR-11 limits the proapoptotic function of its host gene, dE2f1, upon DNA damage by directly modulating a dE2F1-dependent apoptotic transcriptional program PMID: 21856777
  9. report here that dE2F1 promotes ionizing radiation-induced p53-independent apoptosis in larval imaginal discs PMID: 20659447
  10. Screening strategy for studying regulation of the dE2F/RBF pathway in vivo. PMID: 19546319
  11. dE2F1 has a role in nurse cell apoptosis PMID: 12464435
  12. Transcriptional repressor functions of E2F1 & E2F2 cooperate to inhibit genomic DNA synthesis in ovarian follicle cells. The relative functional contributions of E2F1 & E2F2 to gene expression & cell cycle control depend on the developmental context. PMID: 12612083
  13. Data show that dE2F1 is important for the normal pattern of DNA damage-induced apoptosis in Drosophila wing discs. PMID: 16198289
  14. Apoptosis inhibitor-5/antiapoptosis clone-11 (Api5/Aac11) is a critical determinant of dE2F1-induced apoptosis in vivo and in vitro. PMID: 17112319
  15. We examined the process of cell cycle exit in Drosophila wings and eyes and discovered that cell cycle exit can be prevented or even reversed in terminally differentiating cells by the simultaneous activation of E2F1 and either CycE/cdk2 or CycD/cdc4. PMID: 17419999
  16. In addition to de2f2, the loss of a DEAD box protein Belle (Bel) also rescues proliferation of de2f1 mutant cells. PMID: 17923695
  17. p53 is required for dE2F-dependent apoptosis in the response to DNA damage, but it is not required for E2F-dependent apoptosis caused by the inactivation of rbf1. PMID: 18688282
  18. Abnormal expansion of polyQ may potentiate innate androgen-dependent association of AR with Rb through aberrant E2F transactivation caused by suppressed histone deacetylation. PMID: 19237573

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Subcellular Location
Nucleus.
Protein Families
E2F/DP family
Tissue Specificity
Segmentally repeated expression throughout early embryos is restricted to the ventral nerve cord in later embryos.
Database Links

KEGG: dme:Dmel_CG6376

STRING: 7227.FBpp0083516

UniGene: Dm.7299

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