Recombinant Escherichia coli Flagellin (fliC)

Name: Recombinant Escherichia coli Flagellin (fliC)
Brand: CUSABIO
SKU: CSB-EP300968ENV
Price: $388 USD
Availability: InStock
Rating: 5.0 (1 reviews)

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Code	CSB-EP300968ENV
Abbreviation	Recombinant E.coli fliC protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

fliC

Uniprot No.

P04949

Research Area

Immunology

Alternative Names

fliC; flaF; hag; b1923; JW1908Flagellin

Species

Escherichia coli (strain K12)

Source

E.coli

Expression Region

2-498aa

Target Protein Sequence

AQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDDAAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRVRELTVQATTGTNSESDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKNGSMKIQVGANDNQTITIDLKQIDAKTLGLDGFSVKNNDTVTTSAPVTAFGATTTNNIKLTGITLSTEAATDTGGTNPASIEGVYTDNGNDYYAKITGGDNDGKYYAVTVANDGTVTMATGATANATVTDANTTKATTITSGGTPVQIDNTAGSATANLGAVSLVKLQDSKGNDTDTYALKDTNGNLYAADVNETTGAVSVKTITYTDSSGAASSPTAVKLGGDDGKTEVVDIDGKTYDSADLNGGNLQTGLTAGGEALTAVANGKTTDPLKALDDAIASVDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

67.2kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Our Recombinant Escherichia coli (strain K12) fliC protein is a high-quality product tailored to meet the needs of researchers in the field of immunology. The full-length Flagellin protein, derived from the Escherichia coli (strain K12) species, encompasses an expression region of 2-498aa.

Expressed in E. coli, this protein is engineered with an N-terminal 6xHis-SUMO tag for ease of detection and purification. The Recombinant Escherichia coli (strain K12) fliC protein exhibits a purity greater than 90% as determined by SDS-PAGE and is supplied as a lyophilized powder, ensuring a consistent and reliable resource for your research endeavors.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

Submit a Review here

Applications : Antigen, Binding assay/Protein-protein interaction

Review: The recombinant FliC protein from Cusabio was tested as a capture antigen for measuring FliC-specific antibody responses in human sera. The protein coupled efficiently to Luminex beads, remained stable during the coupling procedure, and produced reproducible signals across multiple assay runs. Practical Notes Optimal coupling concentration: ~25–50 µg antigen per 1.25 million beads. Assay readouts were stable after several weeks of bead storage at 4 °C in the dark. Protein handled well in aqueous buffers, with no solubility issues during bead conjugation.

By Mehak

Target Background

Function

Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.

Gene References into Functions

Studies indicate that toll-like receptor 5 (TLR5) specifically interacts with flagellin, and is useful as an analytical recognition element for low concentrations of flagellin. PMID: 24881544
Flagellin subunit FliC function as adhesins to piglet epithelial cells invasion. PMID: 23746569
Results showed a reproducible and clear cut classification with molecular polymorphism of gene rfb cluster and flagella gene fliC. PMID: 19656466
Flagellin in E. coli Nissle 1917 is the major stimulatory factor of human beta-defensin. PMID: 17283097
Relations between the diversity of the fliC gene conditioning flagellum protein in E. coli and the source of the strain origin are presented PMID: 17702465
Flagellin expression by uropathogenic Escherichia coli coincides with ascension of the ureters and colonization of the kidney. PMID: 17925449
This study demonstrated that infection of intestinal epithelial cells by A/E pathogens can trigger an array of proinflammatory responses from epithelial cells through both FliC-dependent and -independent pathways. PMID: 18227166
Insertion of Cys-derived thiol residues in the E. coli FliC protein increased Au(III) sorption and reduction onto the surface of the flagellar filament and resulted in the production of stabilized Au(0) nanoparticles of approximately 20-50 nm diameter. PMID: 18819156
Binding of a fliC(H7) mutant O157 strain to rectal epithelium was significantly reduced as was binding of the flagellated wild-type strain following incubation with H7-specific antibodies. PMID: 19016776
Increased IL-8 levels mainly depended on flagellin expression as fliC-negative EPEC did not elicit a significant IL-8 response despite increased mucosal colonization compared with wild-type EPEC. PMID: 19134113
FliC is secreted by the LEE-encoded type III secretion system and stimulates TLR5 signalling but does not confer motility. PMID: 19200386