Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Cardiovascular
Alternative Names
AGP 1; AGP1; AGPT; ANG 1; ANG; ANG-1; ANG1; Angiopoietin-1; Angiopoietin1; ANGP1_HUMAN; ANGPT 1; Angpt1; KIAA0003; OTTHUMP00000227592; OTTHUMP00000227599; OTTHUMP00000227600
Species
Homo sapiens (Human)
Expression Region
16-498aa
Target Protein Sequence
HIGCSNQRRSPENSGRRYNRIQHGQCAYTFILPEHDGNCRESTTDQYNTNALQRDAPHVEPDFSSQKLQHLEHVMENYTQWLQKLENYIVENMKSEMAQIQQNAVQNHTATMLEIGTSLLSQTAEQTRKLTDVETQVLNQTSRLEIQLLENSLSTYKLEKQLLQQTNEILKIHEKNSLLEHKILEMEGKHKEELDTLKEEKENLQGLVTRQTYIIQELEKQLNRATTNNSVLQKQQLELMDTVHNLVNLCTKEGVLLKGGKREEEKPFRDCADVYQAGFNKSGIYTIYINNMPEPKKVFCNMDVNGGGWTVIQHREDGSLDFQRGWKEYKMGFGNPSGEYWLGNEFIFAITSQRQYMLRIELMDWEGNRAYSQYDRFHIGNEKQNYRLYLKGHTGTAGKQSSLILHGADFSTKDADNDNCMCKCALMLTGGWWFDACGPSNLNGMFYTAGQNHGKLNGIKWHYFKGPSYSLRSTTMMIRPLDF
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 16-498 constitute the expression domain of recombinant Human ANGPT1. This ANGPT1 protein is theoretically predicted to have a molecular weight of 59.9 kDa. The ANGPT1 protein was expressed in e.coli. Fusion of the N-terminal 6xHis tag into the ANGPT1 encoding gene fragment was conducted, allowing for easier detection and purification of the ANGPT1 protein in subsequent stages.
Human angiopoietin-1 (ANGPT1) is a crucial signaling protein in angiogenesis, acting on endothelial cells to regulate blood vessel formation and stability. It binds to the Tie-2 receptor, promoting vascular maturation and integrity. In cardiovascular research, ANGPT1 is essential for vascular development, and its dysregulation is implicated in vascular diseases. In regenerative medicine, ANGPT1 holds the potential for promoting tissue repair through angiogenesis. Additionally, ANGPT1 plays a role in cancer biology, influencing tumor angiogenesis. Investigating ANGPT1 provides insights into vascular biology, cardiovascular diseases, tissue regeneration, and cancer progression, offering avenues for therapeutic interventions in conditions related to angiogenesis and vascular homeostasis.