Recombinant Human Apoptosis-inducing factor 3 (AIFM3)

Code CSB-YP853472HU
MSDS
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Source Yeast
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Code CSB-EP853472HU
MSDS
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Source E.coli
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Code CSB-EP853472HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP853472HU
MSDS
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Source Baculovirus
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Code CSB-MP853472HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AIFM3
Uniprot No.
Alternative Names
AIFL; Aifm3; AIFM3_HUMAN; Apoptosis inducing factor 3; Apoptosis inducing factor like; Apoptosis inducing factor like protein; Apoptosis inducing factor; mitochondrion associated; Apoptosis-inducing factor 3; Apoptosis-inducing factor-like protein; FLJ30473; FLJ45137
Species
Homo sapiens (Human)
Expression Region
1-605
Target Protein Sequence
MGGCFSKPKP VELKIEVVLP EKERGKEELS ASGKGSPRAY QGNGTARHFH TEERLSTPHP YPSPQDCVEA AVCHVKDLEN GQMREVELGW GKVLLVKDNG EFHALGHKCP HYGAPLVKGV LSRGRVRCPW HGACFNISTG DLEDFPGLDS LHKFQVKIEK EKVYVRASKQ ALQLQRRTKV MAKCISPSAG YSSSTNVLIV GAGAAGLVCA ETLRQEGFSD RIVLCTLDRH LPYDRPKLSK SLDTQPEQLA LRPKEFFRAY GIEVLTEAQV VTVDVRTKKV VFKDGFKLEY SKLLLAPGSS PKTLSCKGKE VENVFTIRTP EDANRVVRLA RGRNVVVVGA GFLGMEVAAY LTEKAHSVSV VELEETPFRR FLGERVGRAL MKMFENNRVK FYMQTEVSEL RGQEGKLKEV VLKSSKVVRA DVCVVGIGAV PATGFLRQSG IGLDSRGFIP VNKMMQTNVP GVFAAGDAVT FPLAWRNNRK VNIPHWQMAH AQGRVAAQNM LAQEAEMSTV PYLWTAMFGK SLRYAGYGEG FDDVIIQGDL EELKFVAFYT KGDEVIAVAS MNYDPIVSKV AEVLASGRAI RKREVELFVL HSKTGDMSWL TGKGS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Induces apoptosis through a caspase dependent pathway. Reduces mitochondrial membrane potential.
Gene References into Functions
  1. Upregulated expression of AIFM3 is associated with cholangiocarcinoma. PMID: 27473083
  2. The expression of apoptosis-inducing factor was identified in pineal gland and thymus, but it did not change with age. PMID: 22550867
  3. AIFM3 is a direct target of miR-210 in human hepatoma cells. PMID: 22387901
  4. AIFL has 598 amino acids, with a characteristic Rieske domain and a pyridine nucleotide-disulfide oxidoreductase domain (Pyr_redox). AIFL shares 35% homology with AIF, mainly in the Pyr_redox domain. PMID: 15764604
  5. AIF maintains the transformed state of colon cancer cells through its NADH oxidase activity, by mechanisms that involve complex I function. PMID: 16001080
  6. H. pylori triggers apoptosis in AGS cells via interaction with death receptors in the plasma membrane, leading to the cleavage of procaspase-8, release of cytochrome c and AIF from mitochondria, and activation of subsequent downstream apoptotic events PMID: 19166416

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Subcellular Location
Mitochondrion. Note=Does not translocate to the nucleus upon induction of apoptosis.
Protein Families
FAD-dependent oxidoreductase family
Tissue Specificity
Ubiquitous. Expressed in bone marrow, cerebral cortex, liver, ovary, thymus, thyroid gland and tongue (at protein level).
Database Links

HGNC: 26398

OMIM: 617298

KEGG: hsa:150209

STRING: 9606.ENSP00000382120

UniGene: Hs.723246

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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