Recombinant Human Atrial natriuretic peptide receptor 1(NPR1),partial

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Code CSB-EP016023HU
Size US$1726Purchase it in Cusabio online store
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP016023HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) NPR1.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP016023HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) NPR1.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names NPR1
Uniprot No. P16066
Research Area Cardiovascular
Alternative Names ANP-A; ANPa; ANPR-A; ANPRA; ANPRA_HUMAN; Atrial natriuretic peptide A type receptor; Atrial natriuretic peptide receptor 1; Atrial natriuretic peptide receptor A; Atrial natriuretic peptide receptor type A; Atrionatriuretic peptide receptor A; GC A; GC-A; Guanylate cyclase A; Guanylate cyclase; GUC2A; GUCY2A; Natriuretic peptide A type receptor; Natriuretic Peptide Receptor A; Natriuretic peptide receptor A/guanylate cyclase A; NPR-A; NPR1; NPRA
Species Homo sapiens (Human)
Source E.coli
Expression Region 33-473aa
Target Protein Sequence GNLTVAVVLPLANTSYPWSWARVGPAVELALAQVKARPDLLPGWTVRTVLGSSENALGVCSDTAAPLAAVDLKWEHNPAVFLGPGCVYAAAPVGRFTAHWRVPLLTAGAPALGFGVKDEYALTTRAGPSYAKLGDFVAALHRRLGWERQALMLYAYRPGDEEHCFFLVEGLFMRVRDRLNITVDHLEFAEDDLSHYTRLLRTMPRKGRVIYICSSPDAFRTLMLLALEAGLCGEDYVFFHLDIFGQSLQGGQGPAPRRPWERGDGQDVSARQAFQAAKIITYKDPDNPEYLEFLKQLKHLAYEQFNFTMEDGLVNTIPASFHDGLLLYIQAVTETLAHGGTVTDGENITQRMWNRSFQGVTGYLKIDSSGDRETDFSLWDMDPENGAFRVVLNYNGTSQELVAVSGRKLNWPLGYPPPDIPKCGFDNEDPACNQDHLSTLE
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 64.9kDa
Protein Length Extracellular Domain
Tag Info N-terminal 6xHis-SUMO-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.
Gene References into Functions
  1. These results of the distinct presence of NPRA and NPRBpositive cells in unstable plaques underlying acute myocardial infarction suggested that natriuretic peptides serve a role in regulating plaque instability in humans. PMID: 28765884
  2. The results indicate that the inhibition of NPRA suppresses gastric cancer development and targeting NPRA may represent a promising strategy for the treatment of gastric cancer. PMID: 27634171
  3. Data suggest that signaling via ANP/ANPR (atrial natriuretic factor/ANP receptor) in vascular endothelial cells activates PAK4 (p21-activated kinase 4) and CCM2 (cerebral cavernous malformation 2 protein), resulting in phosphorylation of MLC (myosin light chain), cytoskeletal reorganization, and cell spreading; kinase homology domain of ANPRA (guanylyl cyclase-A) activates downstream targets of ANP/ANPR signaling. PMID: 28432261
  4. After ligand binding, NPRA is rapidly internalized and trafficked from the cell surface into endosomes, Res and lysosomes, with concurrent generation of intracellular cyclic GMP in HEK-293 cells. PMID: 26374856
  5. Data suggest up-regulation of NPRA in skeletal muscle (SM) correlates with whole-body insulin sensitivity; NPRA is down-regulated in SM in obese subjects compared to normal controls; NPRA in SM is up-regulated in response to diet-induced weight loss. PMID: 26253614
  6. In this review we highlight the mechanisms by which NPR-A affects signaling pathways involved in inflammation and cancer, and we discuss its potential as a novel target in inflammation, cancer, and cancer-related inflammation PMID: 25955817
  7. NPRA was detected in E11.5 cardiac progenitor cells. PMID: 25631869
  8. Cardiac fibrosis and the endogenous natriuretic peptide system were evaluated in end-stage heart failure to assess the anti-fibrotic actions of the dual GC-A/-B activator. PMID: 25117468
  9. High NPR1 expression is associated with esophageal squamous cell carcinomas. PMID: 24885858
  10. Molecular dynamics analysis indicated decreases in the values of Van der Waals, electrostatic energy and potential energy of NPRB/Vasonatrin peptide compared to NPRA/Vasonatrin peptide. PMID: 24699414
  11. ACNP stimulated both human natriuretic peptide receptors (NPRs), NPRA and NPRB, as potent as their native ligands in receptor transfected cells. PMID: 23186809
  12. Guanylyl cyclases A and B are asymmetric dimers that are allosterically activated by ATP binding to the catalytic domain. PMID: 22949736
  13. human ventricular cardiomyocytes express low levels of GC-A and GC-A in cardiomyocytes from failing human hearts is refractory to ANP stimulation. PMID: 22133375
  14. Results demonstrate the opposite effects of NPR-A and NPR-C in LPS-mediated endothelial permeability and lung injury. PMID: 22001395
  15. Cellular exposure to Go6976 reduced basal and natriuretic peptide-dependent, but not detergent-dependent, GC-A and GC-B activity. PMID: 21366551
  16. Go6976 reduces GTP binding to the catalytic site of GC-A and GC-B and that ATP increases the magnitude of the inhibition. PMID: 21828054
  17. Midregional pro-A-type natriuretic peptide has a comparable accuracy to brain natriuretic peptide BNP for evaluation of cardiocirculatory exercise limitation. PMID: 20034689
  18. NPRA promotes prostate cancer development in part by regulating macrophage migration inhibitory factor PMID: 21586128
  19. review attempts to provide insights and to delineate the current concepts in the field of functional genomics and signaling of GC-A/NPRA in hypertension and cardiovascular disease states at the molecular level PMID: 21375691
  20. we identified and characterized new phosphorylation sites in GC-A and GC-B and provide the first evidence of phosphorylation sites within human guanylyl cyclases. PMID: 20977274
  21. The SS genotype of the AGT gene was related with an increased risk for 3-vessel CAD PMID: 20529973
  22. kinetics of internalization, trafficking, and down-regulation of recombinant guanylyl cyclase/natriuretic peptide receptor-A (NPRA) PMID: 11704663
  23. unlikely that NPR1 is the same as the MCKD1 gene, mutations in which cause the adult form of medullary cystic kidney disease type 1; however, it is presently unknown whether it plays a disease modifying role PMID: 11851379
  24. This gene codes for natriuretic peptide receptor A, which mediates natriuretic, diuretic, and vasorelaxing actions of the natriuretic peptides. PMID: 12483301
  25. The "deletion 15129" variant might participate in the functional impairment of natriuretic peptide system defining and increased genetic susceptibility to hypertension. PMID: 12506509
  26. activation stabilizes a membrane-distal dimer interface PMID: 12547834
  27. Human dendritic cells, but not monocytes, express functional ANP receptor GC-A at both mRNA and protein levels. PMID: 12794112
  28. NPRA-PKG association may have a role in compartmentation of cGMP-mediated signaling and regulation of receptor sensitivity PMID: 12855709
  29. The novel Npr1 gene 3C variant and the Npr3 gene C(-55) allele are associated with hypertensive family history PMID: 12872042
  30. the M341I missense mutation is associated with risk of myocardial infarct (MI) and may be a genetic marker of MI PMID: 14646971
  31. NPR-A and NPR-B are desensitized in cells in which they are not internalized. PMID: 15459247
  32. discussion of experimental insights into the mechanisms that cells utilize in modulating the delivery and metabolic processing of ligand-bound NPRA into the cell interior [review] PMID: 15911067
  33. discussion of the functional significance of the promoter region of Npr1 gene and its transcriptional regulation [review] PMID: 15911069
  34. ATP does not activate NPRA and NPRB as has been repeatedly reported. Instead, ATP increases activity primarily by maintaining proper receptor phosphorylation status but also serves a previously unappreciated enzyme stabilizing function. PMID: 15911610
  35. interaction of atrial natriuretic peptide with natriuretic peptide receptor-A modulates the binding of ATP to the kinase homology domain PMID: 16262696
  36. NPR-A protein localized to cardiomyocytes, endocardial endothelial cells, and smooth muscle of intramyocardial vessels; downregulation in the density of NPR-A in heart and coronary was observed in artery of patients with ischemic heart disease PMID: 16778132
  37. Data suggest that natriuretic peptide receptor A (NPR-A) interacts with fibronectin to enhance brain natriuretic peptide (BNP) activation of cyclic GMP and that a small NPR-A-specific RGD peptide augments this action of BNP. PMID: 16986166
  38. In patients with Dukes stage C/D colorectal cancer, the Cox Proportional Hazard Model analysis demonstrated a significant association between increased patient survival and low expression of ITGB2 (p = 0.011) and NPR1 (p = 0.023) genes. PMID: 18306933
  39. Real-time PCR revealed that large adipocytes expressed higher mRNA levels of NPRA, and binding studies showed that large adipocytes expressed more NPR-A on the membrane than small adipocytes. PMID: 18383440
  40. The "4-minus" haplotype of the NPR-A receptor gene is associated with high NT-proBNP values and is a genetic determinant of the interindividual variability in the BNP system in healthy individuals but probably not in patients with cardiovascular disease. PMID: 18436476
  41. In the present study conducted in a homogeneous Hellenic population, no associations between Angiotensinogen,angiotensin-converting enzyme, and NPRA gene polymorphisms and hypertension were found. PMID: 19126660
  42. These results indicate that the ANP/GC-A system is involved in immune regulation through plasmacytoid dendritic cells in secondary lymphoid organs. PMID: 19563399

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Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families Adenylyl cyclase class-4/guanylyl cyclase family
Database Links

HGNC: 7943

OMIM: 108960

KEGG: hsa:4881

STRING: 9606.ENSP00000357669

UniGene: Hs.490330

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