Recombinant Human Chitinase-3-like protein 1(CHI3L1)

In Stock
Code CSB-EP005346HU
Size US$1726Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP005346HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) CHI3L1.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP005346HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) CHI3L1.

Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names CHI3L1
Uniprot No. P36222
Research Area Signal Transduction
Alternative Names 39 kDa synovial protein Cartilage glycoprotein 39
Species Homo sapiens (Human)
Source E.coli
Expression Region 22-383aa
Target Protein Sequence YKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 60.5kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA Please contact us to get it.
Still Have Questions? Leave a Message or Start an on-line Chat

Q&A and Customer Reviews

 Q&A
Q:

If I order Lyophilized form.
What should they use for solvent

A:
Very nice to receive your inquiry. Reconstitution of lyophilized protein:
* The reconstitution method is the same for all proteins. Please use deionized sterile water to reconstitute proteins. (Dissolve completely. The concentration could depend on customers' experiment demand.)
* It is better that concentration does not be lower than 0.1mg/ml. Proteins will be unstable if they're too diluted. Generally, we recommend 0.1-1.0mg/ml concentration.
If you have special requirements for concentration, then you could dilute the protein according to their experiment demand.
* We haven’t studied whether the optimal concentration range will vary among different hosts such as E.coli, yeast, mammalian or baculovirus, so we can’t provide useful advice
* If you will use immediately after dissolving one protein, then you don't need to add glycerol. If you won't use the protein in short term, we suggest to add some glycerol for storage.
We suggest you to reconstitute suitable amount of protein and store the remaining protein in lyophilized powder.
You could remark "The lyophilized powder should be packed into multiple vials" when placing order. The amount of lyophilized powder in each vial will depend on your experiment demand.
* In principle, the final concentration of glycerol from 5% to 50% all will be OK. Our default final concentration of glycerol is 50%. You could use it as reference.
 Customer Reviews

Target Data

Function Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.
Involvement in disease Asthma-related traits 7 (ASRT7); Schizophrenia (SCZD)
Subcellular Location Secreted, extracellular space, Cytoplasm, Cytoplasm, perinuclear region, Endoplasmic reticulum
Protein Families Glycosyl hydrolase 18 family
Tissue Specificity Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Very low or undetectable expression in non-inflammatory colon. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts.
Database Links

HGNC: 1932

OMIM: 181500

KEGG: hsa:1116

STRING: 9606.ENSP00000255409

UniGene: Hs.382202

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