Recombinant Human Ciliary neurotrophic factor receptor subunit alpha (CNTFR)

Code CSB-MP005684HU
Abbreviation Recombinant Human CNTFR protein
MSDS
Size $102
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • The purity of CNTFR was greater than 95% as determined by SEC-HPLC
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Product Details

Purity
Greater than 95% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.
Activity
Not Test
Target Names
Uniprot No.
Research Area
Neuroscience
Alternative Names
CNTF receptor subunit alpha;CNTFR-alpha
Source
Mammalian cell
Expression Region
23-342aa
Target Protein Sequence
QRHSPQEAPHVQYERLGSDVTLPCGTANWDAAVTWRVNGTDLAPDLLNGSQLVLHGLELGHSGLYACFHRDSWHLRHQVLLHVGLPPREPVLSCRSNTYPKGFYCSWHLPTPTYIPNTFNVTVLHGSKIMVCEKDPALKNRCHIRYMHLFSTIKYKVSISVSNALGHNATAITFDEFTIVKPDPPENVVARPVPSNPRRLEVTWQTPSTWPDPESFPLKFFLRYRPLILDQWQHVELSDGTAHTITDAYAGKEYIIQVAAKDNEIGTWSDWSVAAHATPWTEEPRHLTTEAQAAETTTSTTSSLAPPPTTKICDPGELGS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Mol. Weight
37.2 kDa
Protein Length
Full Length of Mature Protein
Tag Info
C-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Binds to CNTF. The alpha subunit provides the receptor specificity. Receptor for heterodimeric neurotropic cytokine composed of CLCF1/CLC and CRLF1/CLF-1. Acts as a receptor for the neuroprotective peptide humanin as part of a complex with IL6ST/GP130 and IL27RA/WSX1.
Gene References into Functions
  1. The family-based study showed no association between the CNTFR rs7036351, NET rs3785143 polymorphisms, and attention-deficit hyperactivity disorder. PMID: 24565995
  2. gene expression and surface expression in NK92 cells of ciliary neurotrophic factor alpha (CNTFR-alpha) and glycoprotein (gp)130 in natural killer (NK) cells, in the context of malaria, were investigated. PMID: 23398366
  3. Data indicate that CNTFRalpha plays a role in the formation or maintenance of tumor-initiating cells in gliomas, is a marker that correlates with histological grade, may underlie treatment resistance in some cases, and is a potential therapeutic target. PMID: 23061382
  4. expression of neurotrophin receptors Pan-Trk, p75 neurotrophin receptor (p75(NTR) and ciliary neurotrophic factor receptor-alpha in ulveal melanoma does not show a role in growth. PMID: 22343487
  5. Data show that variants in CNTFR were significantly associated with a lower age at onset of the eating disorders. PMID: 20219210
  6. interactions of CNTFR with LIFR and gp130 in vitro PMID: 12707266
  7. C174T polymorphism in exon 9 of CNTFR is significantly associated with fat-free mass in men and women, with concomitant differences in muscular strength. PMID: 12807897
  8. Structural and docking interaction studies showed that the R197L substitution destabilized the contact site between CLC and CNTFRalpha. PMID: 16782820
  9. In liver CNTFRalpha serves as a key modulator in regulating PI3K and AMP-activated protein kinase dynamically under CNTF stimulation, resulting in the increase of glucose uptake and translocation of glucose transporter 4. PMID: 18307269
  10. These results indicate that humanin protects neurons by binding to a complex or complexes involving CNTFR/WSX-1/gp130. PMID: 19386761

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Subcellular Location
Cell membrane; Lipid-anchor, GPI-anchor.
Protein Families
Type I cytokine receptor family, Type 3 subfamily
Tissue Specificity
Nervous system and skeletal muscle.
Database Links

HGNC: 2170

OMIM: 118946

KEGG: hsa:1271

STRING: 9606.ENSP00000242338

UniGene: Hs.129966

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