CNTFR Antibody

Datasheet
Code CSB-PA005684EA01HU
See More Details
Size US$299
Uniprot No. P26992
Image
  • Immunofluorescence staining of SH-SY5Y cells with CSB-PA005684EA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Western blot
    All lanes: CNTFR antibody at 2µg/ml + Hela whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 41 kDa
    Observed band size: 41 kDa

  • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA005684EA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA005684EA01HU at dilution of 1:100

Immunogen Recombinant Human Ciliary neurotrophic factor receptor subunit alpha protein (106-342AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Relevance Binds to CNTF. The alpha subunit provides the receptor specificity.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Ciliary neurotrophic factor receptor subunit alpha (CNTF receptor subunit alpha) (CNTFR-alpha), CNTFR
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Research Area Neuroscience
Target Names CNTFR
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Binds to CNTF. The alpha subunit provides the receptor specificity.
Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor
Protein Families Type I cytokine receptor family, Type 3 subfamily
Tissue Specificity Nervous system and skeletal muscle.
Database Links

HGNC: 2170

OMIM: 118946

KEGG: hsa:1271

STRING: 9606.ENSP00000242338

UniGene: Hs.129966

Pathway Jak-STAT signaling pathway

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