Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Target Names

CUL5

Uniprot No.

Q93034

Research Area

Cell Biology

Alternative Names

CUL-5;Vasopressin-activated calcium-mobilizing receptor 1;VACM-1

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-780aa

Target Protein Sequence

MATSNLLKNKGSLQFEDKWDFMRPIVLKLLRQESVTKQQWFDLFSDVHAVCLWDDKGPAKIHQALKEDILEFIKQAQARVLSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEITLMGKQGSNKKSNVEDSIVRKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKETILAECQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLLTLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPESKCPELLANYCDMLLRKTPLSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGMPADYVNKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEEFYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAELRRTLWSLVAFPKLKRQVLLYEPQVNSPKDFTEGTLFSVNQEFSLIKNAKVQKRGKINLIGRLQLTTERMREEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQIEWLIEHKYIRRDESDINTFIYMA
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

97.9 kDa

Protein Length

Full Length

Tag Info

C-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. ECS(KLHDC1) complex is part of the DesCEND (destruction via C-end degrons) pathway and mediates ubiquitination and degradation of truncated SELENOS selenoprotein produced by failed UGA/Sec decoding, which ends with a glycine. May form a cell surface vasopressin receptor.; (Microbial infection) Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein.

Gene References into Functions

CUL5 may be an important part of the mechanism for thalidomide-dependent inhibition. PMID: 29746508
These findings report genetic variants possibly associated with susceptibility to HIV-1 infection (CUL5 rs11212495, rs7103534, rs7117111) and partial viral load control (APOBEC3G rs2294367). PMID: 28302043
structural model suggests that the diverse FIV and HIV-1 Vifs use conserved residues for Cullin 5 binding, FIV Vif binds Cullin 5 independently of zinc, in contrast to HIV-1 Vif. PMID: 29263270
these findings identify Cul-5 as a signaling component that connects an LPS-activated TLR4-MyD88 complex to TRAF6 for efficient activation of NF-kappaB PMID: 27233966
CUL5 neddylation may allosterically tune polyubiquitin chain length and topology. PMID: 28082425
Study found that CUL5 expression was significantly decreased in both endometrioid adenocarcinomas with the more aggressive serous type displaying a higher reduction. Its mRNA and protein overexpression in endometrial cancer cells resulted in decreased cell proliferation. PMID: 26847831
Studies indicate that Cullin-RING E3 ubiquitin ligases (CRL) are key players of the ubiquitylation pathway. PMID: 26253693
These results provide important information on the assembly of the Vif-CUL5-E3 ubiquitin ligase and identify a new viV binding interface with CBF-beta at the C-terminus of HIV-1 Vif. PMID: 25424878
Data indicate that cell surface adhesion molecules VCAM-1 and P-selectin play some roles in mechanical stretch-induced HL-60 cell adhesion to mouse common carotid arteries. PMID: 25108430
N-terminal mutants of HIV-1 vif that demonstrated reduced Cul5 binding were also unable to degrade APOBEC3G as well as APOBEC3F. PMID: 24422669
A tumor suppressor gene, CUL5, is identified as a direct target of miR-7 in hepatocellular carcinoma. PMID: 24339204
Silencing CUL5 reduced cellular sensitivity to three distinct HSP90 inhibitors, across four cancer types driven by different protein kinases. PMID: 24760825
In the absence of CBFbeta, Vif does not bind Cul5, thus preventing the assembly of the E3 ligase complex. PMID: 24390320
CBF-beta is critical for the formation of the Vif-ElonginB/ElonginC-Cul5 core E3 ubiquitin ligase complex. PMID: 24390335
data reveal the structural basis for Vif hijacking of the CBF-beta and CUL5 E3 ligase complex, laying a foundation for rational design of novel anti-HIV drugs PMID: 24402281
ASB9 is unstable alone but forms a stable ternary complex with EloBC that binds with high affinity to the Cullin 5 N-terminal domain. PMID: 23837592
crystal structure shows interaction between SOCS2-elongin BC and Cullin-5 PMID: 23897481
Data indicate tht n the Biaka, strong signal of selection was detected at CUL5 and at TSG101. PMID: 23217182
comparison of heat capacity changes supports a model in which CBFbeta prestabilizes Vif((1-192)) relative to Vif((95-192)), consistent with a stronger interaction of Cul5 with Vif's C-terminal Zn(2+)-binding motif. PMID: 23098073
detected a reduced editing associated with the CUL5 SNP6 minor allele and also with certain Vif variants (mutations at sites 46, 122, and 160). PMID: 22145963
Vif alterations may contribute to a rapid AIDS onset and Vif variability could be influenced by APOBEC3G and CUL5 polymorphisms in children PMID: 21571098
study showed VACM-1 DNA in T47D cancer cells is not mutated; SNP found in U138MG, OVCAR-3 and ACHN cell lines results in silent mutation; results suggest in T47D cancer cells, VACM-1 activity may controlled by epigenetic or posttranslational modification PMID: 21635549
Cul5 interacts with the Hsp90 chaperone complex and is recruited to the site of ErbB2 on the plasma membrane, thereby inducing its polyubiquitination and proteasome-mediated degradation. PMID: 19933325
findings report that HIV-1 Vif interacts with cellular proteins Cul5, elongins B and C, and Rbx1 to form an Skp1-cullin-F-box (SCF)-like complex PMID: 14564014
These data indicate that vasopressin-activated calcium-mobilizing (VACM-1) is involved in the regulation of cellular growth. PMID: 15184056
Cul5 promotes vif ubiquination and requires intact SOCS-box PMID: 15574592
the E3 ubiquitin ligase activity of the Vif-BC-Cul5 complex is essential for Vif function against APOBEC3G PMID: 15781449
findings indicate that the assembly of Vif-Cul5 E3 ubiquitin ligases requires a hydrophobic interface within a novel zinc-binding domain in the substrate receptor Vif and a unique region in Cul5 PMID: 16530799
the zinc-binding region in Vif is a novel cullin interaction domain that mediates selective binding to Cul5 PMID: 16636053
Data indicate that in the T47D cancer cell line VACM-1 inhibits growth by attenuating estrogen-dependent/estrogen receptor alpha signaling responses. PMID: 17186378
Adenovirus E4orf6-mediated p53 degradation requires Cul5. PMID: 17351129
CUL5, together with NPAT and PPP2R1B, is implicated in the deregulation of the cell-cycle and apoptosis regulators and in the pathogenesis of B-CLL. PMID: 17449237
Cul5 plays an essential role in regulating neuron migrations during cortical development, possibly by opposing a promigratory effect of Dab1. PMID: 17974915
Degradation of AAV5 proteins can be inhibited by a dominant-negative ubiquitin that prevents chain elongation or by small interfering RNA directed against cullin 5. PMID: 18216112
The purpose of this study was to determine if there is increased expression of Cul5 during granulocytic differentiation of HL-60 cells. PMID: 19118439
HIV-1 virion infectivity factor (Vif) protein-cullin 5 (Cul5) interaction is mediated by zinc binding to the conserved Vif HCCH motif. Zinc enhances the Vif HCCH-Cul5 interaction by 8-fold. PMID: 19588889

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Protein Families

Cullin family

Database Links

HGNC: 2556

OMIM: 601741

KEGG: hsa:8065

STRING: 9606.ENSP00000376808

UniGene: Hs.440320

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Code	CSB-EP835890HU
MSDS	MSDS
Size	$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Cullin-5 (CUL5)

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