Recombinant Human Cystathionine beta-synthase (CBS), partial

Amino acids 2-551 form the expressed segment for recombinant Human CBS. This CBS protein is theoretically predicted to have a molecular weight of 64.5 kDa. Expression of this CBS protein is conducted in e.coli. The N-terminal 6xHis tag was fused into the coding gene segment of CBS, making it easier to detect and purify the CBS recombinant protein in the later stages of expression and purification.

Cystathionine beta-synthase (CBS) is a crucial enzyme primarily involved in the metabolic pathway of homocysteine. One of its most popular and critical research areas focuses on its regulatory role in metabolism and diseases. CBS plays a key role in sulfur amino acid metabolism, participating in the conversion between cysteine and homocysteine, thereby influencing the body's sulfur metabolism balance. Recent studies have shown a widespread interest in the association of CBS with significant diseases, particularly cardiovascular diseases related to hyperhomocysteinemia. Elevated homocysteine levels are closely linked to atherosclerosis and cardiovascular diseases, making CBS a hot topic in research as an essential regulatory factor in this pathway. Additionally, CBS is implicated in various physiological and pathological processes related to the nervous system, tumors, and more. In the nervous system, CBS plays a role in neurotransmitter synthesis and oxidative stress response, garnering attention in the study of diseases associated with the nervous system. In the field of tumors, some studies suggest CBS's involvement in the proliferation and survival mechanisms of tumor cells, providing a new perspective for understanding cancer development.