Recombinant Human DNA replication licensing factor MCM2(MCM2)

Code CSB-EP013590HU
Size US$1726Purchase it in Cusabio online store
(only available for customers from the US)
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names MCM2
Uniprot No. P49736
Research Area Cell Cycle
Alternative Names BM28; CCNL 1; CCNL1; CDC like 1; cdc19; CDCL 1; CDCL1; Cell devision cycle like 1; Cyclin like 1; D3S3194; DNA replication licensing factor MCM2; KIAA0030; MCM 2; MCM2; MCM2 minichromosome maintenance deficient 2 mitotin ; MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae); MCM2 minichromosome maintenance deficient 2; mitotin; MCM2_HUMAN; MGC10606; Minichromosome maintenance complex component 2; Minichromosome maintenance deficient 2 (mitotin); Minichromosome maintenance deficient 2 mitotin ; Minichromosome maintenance protein 2; Minichromosome maintenance protein 2 homolog; Mitotin; Nuclear protein BM28; OTTHUMP00000216047; OTTHUMP00000216050
Species Homo sapiens (Human)
Source E.coli
Expression Region 2-904aa
Target Protein Sequence AESSESFTMASSPAQRRRGNDPLTSSPGRSSRRTDALTSSPGRDLPPFEDESEGLLGTEGPLEEEEDGEELIGDGMERDYRAIPELDAYEAEGLALDDEDVEELTASQREAAERAMRQRDREAGRGLGRMRRGLLYDSDEEDEERPARKRRQVERATEDGEEDEEMIESIENLEDLKGHSVREWVSMAGPRLEIHHRFKNFLRTHVDSHGHNVFKERISDMCKENRESLVVNYEDLAAREHVLAYFLPEAPAELLQIFDEAALEVVLAMYPKYDRITNHIHVRISHLPLVEELRSLRQLHLNQLIRTSGVVTSCTGVLPQLSMVKYNCNKCNFVLGPFCQSQNQEVKPGSCPECQSAGPFEVNMEETIYQNYQRIRIQESPGKVAAGRLPRSKDAILLADLVDSCKPGDEIELTGIYHNNYDGSLNTANGFPVFATVILANHVAKKDNKVAVGELTDEDVKMITSLSKDQQIGEKIFASIAPSIYGHEDIKRGLALALFGGEPKNPGGKHKVRGDINVLLCGDPGTAKSQFLKYIEKVSSRAIFTTGQGASAVGLTAYVQRHPVSREWTLEAGALVLADRGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTSLQARCTVIAAANPIGGRYDPSLTFSENVDLTEPIISRFDILCVVRDTVDPVQDEMLARFVVGSHVRHHPSNKEEEGLANGSAAEPAMPNTYGVEPLPQEVLKKYIIYAKERVHPKLNQMDQDKVAKMYSDLRKESMATGSIPITVRHIESMIRMAEAHARIHLRDYVIEDDVNMAIRVMLESFIDTQKFSVMRSMRKTFARYLSFRRDNNELLLFILKQLVAEQVTYQRNRFGAQQDTIEVPEKDLVDKARQINIHNLSAFYDSELFRMNKFSHDLKRKMILQQF
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 105.8kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
(From Uniprot)
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis.
Gene References into Functions
  1. The findings suggest that Mcm-2 may be a sensitive proliferation marker in oral potentially malignant and malignant lesions which may be useful for differentiating between Verrucous Hyperplasia (VH) with/ without dysplasia, Verrucous Carcinoma (VC) and Oral Squamous Cell Carcinoma (OSCC). PMID: 29661732
  2. High MCM2 expression is associated with HIV-1 infection. PMID: 29084722
  3. The results showed that MUS81 modulates MCM2 levels as well as homologous recombination (HR) activity. Moreover, downregulation of MUS81 increased the sensitivity of epithelial ovarian cancer (EOC)cells to olaparib by inducing S phase arrest and promoting apoptosis through activation of MCM2. MUS81 may be a potential novel therapeutic target for EOC. PMID: 29393493
  4. MCM2 played important roles in regulating cell cycle- and DNA replication-related pathways. E2F could upregulate the expression levels of MCM2 by deregulating the methylations of their promoters to promote the relapse of Acute Lymphoblastic Leukemia . PMID: 29768346
  5. ProEx C is an immunohistochemical cocktail containing antibodies direct against topoisomerase IIalpha (TOP2A) and minichromosome maintenance 2 (MCM2) proteins. This brief review covers the effective utility of ProEx C as adjunct tool in assessing the urothelial lesions in urine cytology, also providing prognostic and therapeutic information to help in clinical decisions. PMID: 28638271
  6. the decreased growth of osteosarcoma cells by MCM2 or MCM3 knockdown was reversed by DHX9 overexpression, indicating that MCM2 and MCM3 activity was DHX9-dependent. PMID: 28460433
  7. Results show that MCM2 is significantly upregulated in urothelial carcinoma. PMID: 27780919
  8. Sld5 a component of GINS complex interacts with SIK1 and recruits it to the sites of DNA replication at the onset of S phase. PMID: 27592030
  9. MCM2 expression in serrated colonic polyps demonstrates aberrant cellular proliferation and changes in the colonic microenvironment may promote adenoma morphogenesis and predisposition to malignancy. PMID: 28302537
  10. these findings suggest that lnc-FTX may act as a tumor suppressor in hepatocellular carcinoma (HCC) through physically binding miR-374a and MCM2. It may also be one of the reasons for HCC gender disparity and may potentially contribute to HCC treatment PMID: 27065331
  11. Data suggest that interaction of Mcm10 with Mcm2-7 multimer requires Mcm10 domain that contains amino acids 530-655, which overlaps with domain required for stable retention of Mcm10 on chromatin; Mcm10 conserved domain (amino acids 200-482) is essential for DNA replication; both conserved domain and Mcm2-7-binding domain are required for full activity of Mcm10. PMID: 28646110
  12. MCM2-MCM6 complex is required for CHK2 chromatin loading and its phosphorylation to DNA damage response in squamous cell carcinoma cells. PMID: 27964702
  13. BD ProExtrade mark C assay containing MCM2 and TOP2A antibodies showed strong specific nuclear staining that correlated with increased cervical dysplasia and lesion severity. PMID: 28093271
  14. these data suggest that positive MCM2 and negative TIP30 expression are closely correlated with the clinical, pathological and biological parameters, in addition to poor prognosis in patients with gallbladder cancer. PMID: 27748889
  15. knockdown of MCM2 or MCM6 could significantly inhibit foci forming of MDC1 in TE-1 nuclei in response to bleomycin-induced DNA damage (p < 0.001). This study indicates the direct interaction between MDC1 and MCMs in TE-1 nuclei. PMID: 27908247
  16. PTEN regulates DNA replication through MCM2 and loss of PTEN function leads to replication defects and genomic instability. PMID: 26549452
  17. MCM2 expression is higher in ovarian and endometrial high-grade serous carcinomas (HGSC) than in ovarian or endometrial endometrioid carcinoma. Knockdown in ovarian HGSC cell line decreased cell proliferation. PMID: 26889980
  18. quaternary complex of histone H3-H4 heterodimer with chaperone ASF1 and the replicative helicase subunit MCM2 PMID: 26186914
  19. MCM2 is a novel gene responsible for nonsyndromic hearing loss of autosomal dominant inheritance in a Chinese family. PMID: 26196677
  20. The findings indicated that Mcm-2 could be a useful marker for early detection of oral squamous cell carcinoma PMID: 26712671
  21. MCM2 may be a novel therapeutic target of lovastatin treatment in NSCLCs. PMID: 25738322
  22. minichromosome maintenance protein-2 may serve as a useful marker in the screening of cervical carcinoma and precancerous lesions and improve the diagnosis of atypical squamous cell of undetermined significance PMID: 25755789
  23. Data indicate that minichromosome maintenance protein 2 (MCM2) binding is not required for incorporation of histone H3.1-H4 into chromatin but is important for stability of H3.1-H4. PMID: 26167883
  24. The data suggest that MCM2 is not a good biomarker when comparing the different clinical stages of cervical cancer. PMID: 24706378
  25. Thermodynamic analysis of the quaternary complex together with structural modeling support that ASF1 and MCM2 could form a chaperoning module for histones H3 and H4 protecting them from promiscuous interactions. PMID: 25618846
  26. Data suggest that minichromosome maintenance complex component 2 (MCM2) is a likely target gene of microRNA-31. PMID: 24970811
  27. Results show that in Glioma patients, MCM 2, MCM3 and MCM7 mRNA are up-regulated and correlated with poor outcome. PMID: 25046975
  28. Of the total, the deregulation of several genes (CDK1, CDK2, CDK4, MCM2, MCM3, MCM4, EIF3a and RPN2) were potentially associated with disease development and progression. PMID: 24386425
  29. MCM2/TOP2A immunohistochemistry can help to support a diagnosis of true dysplasia for patients without a history of head and neck cancer. PMID: 24630889
  30. ORC/Cdc6/MCM2-7 complex is a new regulatory mechanism for the helicase. PMID: 23803736
  31. Mcm2-7 loads onto origins during initiation as a double hexamer, yet does not act as a double-stranded DNA pump during elongation. PMID: 22918583
  32. MCM-2 is a therapeutic target of Trichostatin A in colon cancer cells. PMID: 23770000
  33. Mcm2 expression and DNA ploidy analysis could be used to predict areas of malignant transformation in oral proliferative verrucous leukoplakia. PMID: 23347057
  34. MCM2 is a sensitive, specific and efficient biomarker of gastric cardiac cancer having potential use in the clinic. PMID: 23329420
  35. Immunohistochemical expression of minichromosome maintenance complex protein 2 predicts biochemical recurrence in prostate cancer. PMID: 22554381
  36. The results of this study indicated that the higher MCM-2 expressions in RC than the KCOT might be related to the inflammation and this protein might be more sensitive to inflammation. PMID: 22778705
  37. ProEx C showed the best specificity; p16 displayed the highest sensitivity and area under receiver operating characteristic curve for squamous intraepithelial lesions diagnosis. PMID: 22590819
  38. Data show that using a 200-nt primed circular DNA substrate, the combined action of DNA polymerase epsilon and the Cdc45/Mcm2-7/GINS (CMG complex) leads to the formation of products >10 kb in length. PMID: 22474384
  39. The up-regulated expression of MCM2 is associated with frequent apoptosis in myelodysplastic syndromes (MDS) and may have an important role in the pathogenesis of MDS. PMID: 22115939
  40. ProExC expression is not associated with HPV in squamous cell carcinoma in situ of the skin PMID: 20184667
  41. Our data suggest that MCM2 could serve as a novel prognostic biomarker in gastric carcinoma. PMID: 21947329
  42. Salivary gland carcinomas express maspin and MCM2 with variable levels and cellular localization PMID: 21943228
  43. MCM2 is a promising prognostic factor in wilm's tumor treated according to the SIOP scheme PMID: 21866463
  44. overexpression of MCM2 or loss of expression of Tat-interacting protein 30 is closely related to carcinogenesis, progression, biological behavior, and prognosis of gallbladder adenocarcinoma. PMID: 21543106
  45. Lymph node sections from 138 HL patients were immunohistochemically stained for cyclin D3 (CCND3), MCM2 and MCM7 aiming to investigate clinical outcome. PMID: 21965782
  46. These results suggest that HERC2 regulates DNA replication progression and origin firing by facilitating MCM2 phosphorylation. PMID: 21775519
  47. evaluated the intensity of metallothionein expression in various histological types of non-small cell lung cancer and correlated expression intensity with pathological parameters and Ki-67 and minichromosome maintaince protein 2 proliferation markers PMID: 21868526
  48. MT-I/II may play a key role in IDC proliferation, but is not a useful prognostic factor of this disease PMID: 21868554
  49. lack of MCM2, proteins expression which may explain commonly known low mitotic activity of desmoid tumour cells PMID: 21478101
  50. Study shows that despite the predominantly different localization of MCM 2 and replication signals, there is still a small but significant fraction of MCM 2 proteins that co-localize with DNA replication foci during most of S phase. PMID: 21457648

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Involvement in disease Deafness, autosomal dominant, 70 (DFNA70)
Subcellular Location Nucleus
Protein Families MCM family
Database Links

HGNC: 6944

OMIM: 116945

KEGG: hsa:4171

STRING: 9606.ENSP00000265056

UniGene: Hs.477481

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