MCM2 Antibody

Code CSB-PA013590LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate
    All lanes: MCM2 antibody at 4.8µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 102 kDa
    Observed band size: 102 kDa

  • IHC image of CSB-PA013590LA01HU diluted at 1:600 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of MCF-7 cells with CSB-PA013590LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) MCM2 Polyclonal antibody
Uniprot No. P49736
Target Names MCM2
Alternative Names BM28 antibody; CCNL 1 antibody; CCNL1 antibody; CDC like 1 antibody; cdc19 antibody; CDCL 1 antibody; CDCL1 antibody; Cell devision cycle like 1 antibody; Cyclin like 1 antibody; D3S3194 antibody; DNA replication licensing factor MCM2 antibody; KIAA0030 antibody; MCM 2 antibody; MCM2 antibody; MCM2 minichromosome maintenance deficient 2 mitotin antibody; MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody; MCM2 minichromosome maintenance deficient 2; mitotin antibody; MCM2_HUMAN antibody; MGC10606 antibody; Minichromosome maintenance complex component 2 antibody; Minichromosome maintenance deficient 2 (mitotin) antibody; Minichromosome maintenance deficient 2 mitotin antibody; Minichromosome maintenance protein 2 antibody; Minichromosome maintenance protein 2 homolog antibody; Mitotin antibody; Nuclear protein BM28 antibody; OTTHUMP00000216047 antibody; OTTHUMP00000216050 antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human DNA replication licensing factor MCM2 protein (2-904AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:500-1:1000
IF 1:200-1:500
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Disruption of the polyubiquitin gene Ubb reduces the self-renewal capacity of neural stem cells. CW Park,Biochem Biophys Res Commun,2020

Applications: Immunoblot detection
Review: Immunoblot detection of Ub and Mcm2 in Ubb heterozygous and Ubb knockout NSCs. Band intensities of Mcm2 were normalized to the loading control a-tubulin, and protein levels are shown relative to control levels.
PMID: 32283822

Target Data

Function Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis.
Gene References into Functions
  1. The findings suggest that Mcm-2 may be a sensitive proliferation marker in oral potentially malignant and malignant lesions which may be useful for differentiating between Verrucous Hyperplasia (VH) with/ without dysplasia, Verrucous Carcinoma (VC) and Oral Squamous Cell Carcinoma (OSCC). PMID: 29661732
  2. High MCM2 expression is associated with HIV-1 infection. PMID: 29084722
  3. The results showed that MUS81 modulates MCM2 levels as well as homologous recombination (HR) activity. Moreover, downregulation of MUS81 increased the sensitivity of epithelial ovarian cancer (EOC)cells to olaparib by inducing S phase arrest and promoting apoptosis through activation of MCM2. MUS81 may be a potential novel therapeutic target for EOC. PMID: 29393493
  4. MCM2 played important roles in regulating cell cycle- and DNA replication-related pathways. E2F could upregulate the expression levels of MCM2 by deregulating the methylations of their promoters to promote the relapse of Acute Lymphoblastic Leukemia . PMID: 29768346
  5. ProEx C is an immunohistochemical cocktail containing antibodies direct against topoisomerase IIalpha (TOP2A) and minichromosome maintenance 2 (MCM2) proteins. This brief review covers the effective utility of ProEx C as adjunct tool in assessing the urothelial lesions in urine cytology, also providing prognostic and therapeutic information to help in clinical decisions. PMID: 28638271
  6. the decreased growth of osteosarcoma cells by MCM2 or MCM3 knockdown was reversed by DHX9 overexpression, indicating that MCM2 and MCM3 activity was DHX9-dependent. PMID: 28460433
  7. Results show that MCM2 is significantly upregulated in urothelial carcinoma. PMID: 27780919
  8. Sld5 a component of GINS complex interacts with SIK1 and recruits it to the sites of DNA replication at the onset of S phase. PMID: 27592030
  9. MCM2 expression in serrated colonic polyps demonstrates aberrant cellular proliferation and changes in the colonic microenvironment may promote adenoma morphogenesis and predisposition to malignancy. PMID: 28302537
  10. these findings suggest that lnc-FTX may act as a tumor suppressor in hepatocellular carcinoma (HCC) through physically binding miR-374a and MCM2. It may also be one of the reasons for HCC gender disparity and may potentially contribute to HCC treatment PMID: 27065331
  11. Data suggest that interaction of Mcm10 with Mcm2-7 multimer requires Mcm10 domain that contains amino acids 530-655, which overlaps with domain required for stable retention of Mcm10 on chromatin; Mcm10 conserved domain (amino acids 200-482) is essential for DNA replication; both conserved domain and Mcm2-7-binding domain are required for full activity of Mcm10. PMID: 28646110
  12. MCM2-MCM6 complex is required for CHK2 chromatin loading and its phosphorylation to DNA damage response in squamous cell carcinoma cells. PMID: 27964702
  13. BD ProExtrade mark C assay containing MCM2 and TOP2A antibodies showed strong specific nuclear staining that correlated with increased cervical dysplasia and lesion severity. PMID: 28093271
  14. these data suggest that positive MCM2 and negative TIP30 expression are closely correlated with the clinical, pathological and biological parameters, in addition to poor prognosis in patients with gallbladder cancer. PMID: 27748889
  15. knockdown of MCM2 or MCM6 could significantly inhibit foci forming of MDC1 in TE-1 nuclei in response to bleomycin-induced DNA damage (p < 0.001). This study indicates the direct interaction between MDC1 and MCMs in TE-1 nuclei. PMID: 27908247
  16. PTEN regulates DNA replication through MCM2 and loss of PTEN function leads to replication defects and genomic instability. PMID: 26549452
  17. MCM2 expression is higher in ovarian and endometrial high-grade serous carcinomas (HGSC) than in ovarian or endometrial endometrioid carcinoma. Knockdown in ovarian HGSC cell line decreased cell proliferation. PMID: 26889980
  18. quaternary complex of histone H3-H4 heterodimer with chaperone ASF1 and the replicative helicase subunit MCM2 PMID: 26186914
  19. MCM2 is a novel gene responsible for nonsyndromic hearing loss of autosomal dominant inheritance in a Chinese family. PMID: 26196677
  20. The findings indicated that Mcm-2 could be a useful marker for early detection of oral squamous cell carcinoma PMID: 26712671
  21. MCM2 may be a novel therapeutic target of lovastatin treatment in NSCLCs. PMID: 25738322
  22. minichromosome maintenance protein-2 may serve as a useful marker in the screening of cervical carcinoma and precancerous lesions and improve the diagnosis of atypical squamous cell of undetermined significance PMID: 25755789
  23. Data indicate that minichromosome maintenance protein 2 (MCM2) binding is not required for incorporation of histone H3.1-H4 into chromatin but is important for stability of H3.1-H4. PMID: 26167883
  24. The data suggest that MCM2 is not a good biomarker when comparing the different clinical stages of cervical cancer. PMID: 24706378
  25. Thermodynamic analysis of the quaternary complex together with structural modeling support that ASF1 and MCM2 could form a chaperoning module for histones H3 and H4 protecting them from promiscuous interactions. PMID: 25618846
  26. Data suggest that minichromosome maintenance complex component 2 (MCM2) is a likely target gene of microRNA-31. PMID: 24970811
  27. Results show that in Glioma patients, MCM 2, MCM3 and MCM7 mRNA are up-regulated and correlated with poor outcome. PMID: 25046975
  28. Of the total, the deregulation of several genes (CDK1, CDK2, CDK4, MCM2, MCM3, MCM4, EIF3a and RPN2) were potentially associated with disease development and progression. PMID: 24386425
  29. MCM2/TOP2A immunohistochemistry can help to support a diagnosis of true dysplasia for patients without a history of head and neck cancer. PMID: 24630889
  30. ORC/Cdc6/MCM2-7 complex is a new regulatory mechanism for the helicase. PMID: 23803736
  31. Mcm2-7 loads onto origins during initiation as a double hexamer, yet does not act as a double-stranded DNA pump during elongation. PMID: 22918583
  32. MCM-2 is a therapeutic target of Trichostatin A in colon cancer cells. PMID: 23770000
  33. Mcm2 expression and DNA ploidy analysis could be used to predict areas of malignant transformation in oral proliferative verrucous leukoplakia. PMID: 23347057
  34. MCM2 is a sensitive, specific and efficient biomarker of gastric cardiac cancer having potential use in the clinic. PMID: 23329420
  35. Immunohistochemical expression of minichromosome maintenance complex protein 2 predicts biochemical recurrence in prostate cancer. PMID: 22554381
  36. The results of this study indicated that the higher MCM-2 expressions in RC than the KCOT might be related to the inflammation and this protein might be more sensitive to inflammation. PMID: 22778705
  37. ProEx C showed the best specificity; p16 displayed the highest sensitivity and area under receiver operating characteristic curve for squamous intraepithelial lesions diagnosis. PMID: 22590819
  38. Data show that using a 200-nt primed circular DNA substrate, the combined action of DNA polymerase epsilon and the Cdc45/Mcm2-7/GINS (CMG complex) leads to the formation of products >10 kb in length. PMID: 22474384
  39. The up-regulated expression of MCM2 is associated with frequent apoptosis in myelodysplastic syndromes (MDS) and may have an important role in the pathogenesis of MDS. PMID: 22115939
  40. ProExC expression is not associated with HPV in squamous cell carcinoma in situ of the skin PMID: 20184667
  41. Our data suggest that MCM2 could serve as a novel prognostic biomarker in gastric carcinoma. PMID: 21947329
  42. Salivary gland carcinomas express maspin and MCM2 with variable levels and cellular localization PMID: 21943228
  43. MCM2 is a promising prognostic factor in wilm's tumor treated according to the SIOP scheme PMID: 21866463
  44. overexpression of MCM2 or loss of expression of Tat-interacting protein 30 is closely related to carcinogenesis, progression, biological behavior, and prognosis of gallbladder adenocarcinoma. PMID: 21543106
  45. Lymph node sections from 138 HL patients were immunohistochemically stained for cyclin D3 (CCND3), MCM2 and MCM7 aiming to investigate clinical outcome. PMID: 21965782
  46. These results suggest that HERC2 regulates DNA replication progression and origin firing by facilitating MCM2 phosphorylation. PMID: 21775519
  47. evaluated the intensity of metallothionein expression in various histological types of non-small cell lung cancer and correlated expression intensity with pathological parameters and Ki-67 and minichromosome maintaince protein 2 proliferation markers PMID: 21868526
  48. MT-I/II may play a key role in IDC proliferation, but is not a useful prognostic factor of this disease PMID: 21868554
  49. lack of MCM2, proteins expression which may explain commonly known low mitotic activity of desmoid tumour cells PMID: 21478101
  50. Study shows that despite the predominantly different localization of MCM 2 and replication signals, there is still a small but significant fraction of MCM 2 proteins that co-localize with DNA replication foci during most of S phase. PMID: 21457648
  51. Minichromosome maintenance protein 2 is more sensitive and specific than proliferating cell nuclear antigen and Ki67 in indicating esophageal dysplasia. PMID: 21237484
  52. study demonstrated a positive correlation between expressions of Ki-67 and MCM-2 in ductal breast cancers; most intense expression of these two markers was demonstrated in G3 grade cancers PMID: 21097442
  53. MCM-2 expression did not show significant associations with any clinicopathological parameters, while Ki-67 expression was merely significantly associated with tumor size. Neither MCM-2 nor Ki-67 expression had significant prognostic value. PMID: 20694513
  54. Suggest that MCM 2 protein is a reliable marker for diagnosing and grading actinic keratosis and further could be hypothesized as an important prognostic factor. PMID: 20847638
  55. deregulation of MCM2, MCM3 and MCM7 expression might be involved in medulloblastoma tumorigenesis PMID: 20661220
  56. MCM2 is a component of the minichromosome maintenance (MCM) complex, which is essential for the initiation and elongation of DNA during replication in eukaryotic cells. PMID: 20697251
  57. the ability of cytomegalovirus to delay the accumulation of the mini-chromosome maintenance (MCM) complex proteins, represented by MCM2 and MCM4, and prevent their loading onto chromatin, was compromised in the absence of pUL117 PMID: 20333247
  58. Mcm2 index showed a significant association with pituitary tumor extension (p = 0.02), but not with tumor invasion. PMID: 20174894
  59. MCM2 expression was observed in the basal third to half of the colonic crypts in normal mucosa, but throughout colonic adenocarcinoma and adenoma epithelia. However, expression of MCM2 in adenocarcinomas was significantly higher than in adenomas. PMID: 18803052
  60. MCM2 is a sensitive marker for premalignant lung cells since it is present in cells at the surface of metaplastic lung lesions, which are more likely to be exfoliated into sputum. PMID: 11472637
  61. MCM2(Minichromosome Maintenance 2) is regulated via a P53-independent pathway, and a useful biomarker of proliferating cells. PMID: 11872961
  62. MCM2 expression in fibrous histiocytoma correlates with cell proliferation.Its expression is ubiquitous in proliferating cells, regardless of the expression of P53. MCM2 might be a reliable marker of proliferating cells in human MFH. PMID: 12119552
  63. role of AKAP95 in DNA replication by providing a scaffold for MCM2 PMID: 12740381
  64. Increased expression of minichromosome maintenance protein-2 and Ki67 is associated with laryngeal squamous epithelial lesions PMID: 12966424
  65. ATM/ATR-dependent (ataxia-telangiectasia-mutated/ATM- and Rad3-related) checkpoint pathways are directly linked to three members of the MCM complex(MCM2,MCM3,MCM7). PMID: 15210935
  66. pairwise interactions of individual human MCM subunits by using the yeast two-hybrid system and in vivo protein-protein crosslinking PMID: 15236977
  67. purified hRad51 and hRad52 interact with each other as well as with Mini chromosome maintenance (MCM) proteins in HeLa cell extracts PMID: 15766559
  68. the majority of the Mcm2 isoforms phosphorylated by Cdc7 are not stably associated with chromatin PMID: 16446360
  69. The MCM2 protein expression was associated with the highest levels of proliferation, suggesting a synergistic effect. PMID: 16609045
  70. The combination of MCM2, geminin and Ki67 could represent a valuable tool in the understanding of hepatocellular carcinoma progression in cirrhosis. PMID: 16629645
  71. MCM2 is a helpful marker for differentiating follicular carcinoma and follicular adenoma of the thyroid. PMID: 16722928
  72. Results describe the mapping of the sites in human Mcm2 protein that are phosphorylated by Cdc7. PMID: 16864800
  73. higher expression in MCM2 and gelsolin was significantly associated with poorer prognosis in patients with NSCLC, which suggests that higher tumor proliferation and motility may be important in the prognosis of NSCLC PMID: 16882345
  74. Taken together, these results indicate that Cdc7/Dbf4 phosphorylation of MCM2 is essential for the initiation of DNA replication in mammalian cells. PMID: 16899510
  75. Immunoreactivity for p16, MCM2, and TOP IIA was detected in only 76% of cases in low-grade squamous intraepitelial lesions (LSIL). PMID: 17512033
  76. MCM2 is part of the GINS complex. PMID: 17557111
  77. MCM-2 and MCM-5 proteins appear to be promising as prognostic markers in patients with ovarian adenocarcinomas. PMID: 17940502
  78. exogenous Cdt1 induces re-replication by de-repressing endogenous Cdt1 through the titration of PCNA and cyclin; Cdt1 lacking the evolutionarily conserved region that interacts with MCM2-7 is capable of inducing re-replication PMID: 18184650
  79. Results strongly suggest that the MCM2 fragments derived from the C-terminal region inhibit DNA helicase activity through their ability to bind to ssDNA. PMID: 18190532
  80. may be a sensitive proliferation marker in salivary gland tumours PMID: 18248354
  81. MCM protein 2 is present in melanocytes from benign nevi, dysplastic nevi, primary cutaneous melanomas, and cutaneous melanoma metastases. PMID: 18249467
  82. Cdc7/Dbf4 kinase activity inhibition affects specific phosphorylation sites on MCM2 in cancer cells PMID: 18286467
  83. In colon cancer, MCM2 expression was associated with the tumors' histological grade, existence of nodular metastases, malignancy on adenoma, and vascular invasion. PMID: 18465232
  84. Minichromosome maintenance protein 2 is a useful proliferative marker of breast carcinoma. PMID: 18774496
  85. MCM-2 fragment is upregulated in senescence, quiescence, and differentiation PMID: 19001846
  86. absence of multiple MCM2 transcripts implied a possible posttranslational molecular cleavage in generation of the MCM2-related fragment, and a potential functional role in the regulation of the activity of the MCM protein complex. PMID: 19001876
  87. The potential value of MCM2 in early diagnosis of colorectal cancer. PMID: 19077563
  88. MCM7 is a more reliable marker than MCM2 or Ki-67 in lung adenocarcinomas PMID: 19144445
  89. Lymphocyte Mcm-2 expression is a useful adjunct to histology in differentiating between hepatitis C virus graft infection and acute cellular rejection. PMID: 19243005
  90. the MCM2-7 proteins are co-localized with RNA Pol II on chromatins of constitutively transcribing genes, and MCM5 is required for transcription elongation of RNA Pol II. PMID: 19318354
  91. MCM2 does not appear useful in differentiating basal cell carcinomas with follicular differentiation from trichoepitheliomas PMID: 19522849
  92. Phosphorylation of Mcm2 by Cdc7 kinase promotes chromatin loading of Mcm2. PMID: 19647517
  93. Replisome factors MCM10, MCM2and MCM7 helicase, are the primary interaction partner proteins of human RECQ4. PMID: 19696745
  94. interactions between Cdc45, Mcm2-7, and the GINS complex (collectively called the CMG complex), which seem to play a key role in the formation and progression of replication forks PMID: 19805216
  95. Functional analysis of the Xenopus homolog PMID: 11864598
  96. Functional analysis of the mouse homolog PMID: 11568184

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Involvement in disease Deafness, autosomal dominant, 70 (DFNA70)
Subcellular Location Nucleus
Protein Families MCM family
Database Links

HGNC: 6944

OMIM: 116945

KEGG: hsa:4171

STRING: 9606.ENSP00000265056

UniGene: Hs.477481

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