Recombinant Human Glycylpeptide N-tetradecanoyltransferase 2 (NMT2)

Code CSB-YP015901HU
MSDS
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Source Yeast
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Code CSB-EP015901HU
MSDS
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Source E.coli
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Code CSB-EP015901HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015901HU
MSDS
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Source Baculovirus
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Code CSB-MP015901HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NMT2
Uniprot No.
Alternative Names
EC 2.3.1.97; Glycylpeptide N tetradecanoyltransferase 2; Glycylpeptide N-tetradecanoyltransferase 2; Myristoyl CoA:protein N myristoyltransferase 2; Myristoyl-CoA:protein N-myristoyltransferase 2; N-myristoyltransferase 2; NMT 2; NMT2; NMT2_HUMAN; Peptide N myristoyltransferase 2; Peptide N-myristoyltransferase 2; Type II N myristoyltransferase ; Type II N-myristoyltransferase
Species
Homo sapiens (Human)
Expression Region
1-498
Target Protein Sequence
MAEDSESAAS QQSLELDDQD TCGIDGDNEE ETEHAKGSPG GYLGAKKKKK KQKRKKEKPN SGGTKSDSAS DSQEIKIQQP SKNPSVPMQK LQDIQRAMEL LSACQGPARN IDEAAKHRYQ FWDTQPVPKL DEVITSHGAI EPDKDNVRQE PYSLPQGFMW DTLDLSDAEV LKELYTLLNE NYVEDDDNMF RFDYSPEFLL WALRPPGWLL QWHCGVRVSS NKKLVGFISA IPANIRIYDS VKKMVEINFL CVHKKLRSKR VAPVLIREIT RRVNLEGIFQ AVYTAGVVLP KPIATCRYWH RSLNPRKLVE VKFSHLSRNM TLQRTMKLYR LPDVTKTSGL RPMEPKDIKS VRELINTYLK QFHLAPVMDE EEVAHWFLPR EHIIDTFVVE SPNGKLTDFL SFYTLPSTVM HHPAHKSLKA AYSFYNIHTE TPLLDLMSDA LILAKSKGFD VFNALDLMEN KTFLEKLKFG IGDGNLQYYL YNWRCPGTDS EKVGLVLQ
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins.
Gene References into Functions
  1. ligand binding properties of the NMT/ACBD6 complex can explain how the NMT reaction can proceed in the presence of the very abundant competitive substrate, C(16)-CoA. PMID: 26621918
  2. induction of NMT2 may play a central role in dioxin carcinogenicity. PMID: 11731439
  3. NMT1 and NMT2 have only partially overlapping functions; NMT1 is critical for tumor cell proliferation PMID: 16123142
  4. Involved in progression and regulation of telomerase PMID: 16364039
  5. Protein-protein interaction of NMTs revealed that m-calpain interacts with NMT1 while caspase-3 interacts with NMT2. PMID: 16530191
  6. Disruption of the gene for NMT2 by a t(8;10) translocation in a male with hypogonadism. PMID: 17568424
  7. Nef is preferentially myristoylated by NMT2, suggesting that selective inhibition of NMT2 may provide a novel means of blocking HIV virulence. PMID: 18089753

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Subcellular Location
Cytoplasm. Membrane; Peripheral membrane protein.
Protein Families
NMT family
Database Links

HGNC: 7858

OMIM: 603801

KEGG: hsa:9397

STRING: 9606.ENSP00000367407

UniGene: Hs.60339

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