Recombinant Human GrpE protein homolog 1, mitochondrial (GRPEL1)

Code CSB-YP875707HU
MSDS
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Source Yeast
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Code CSB-EP875707HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP875707HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP875707HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GRPEL1
Uniprot No.
Alternative Names
FLJ25609; GREPEL 1; GREPEL1; GrpE like 1; GrpE like 1 mitochondrial; GrpE like protein cochaperone; GrpE protein homolog 1 mitochondrial; GrpE protein homolog 1; mitochondrial; GRPE1_HUMAN; GRPEL 1; Grpel1; HMGE; Mt GrpE#1; Mt-GrpE#1
Species
Homo sapiens (Human)
Expression Region
28-217
Target Protein Sequence
CTA TKQKNSGQNL EEDMGQSEQK ADPPATEKTL LEEKVKLEEQ LKETVEKYKR ALADTENLRQ RSQKLVEEAK LYGIQAFCKD LLEVADVLEK ATQCVPKEEI KDDNPHLKNL YEGLVMTEVQ IQKVFTKHGL LKLNPVGAKF DPYEHEALFH TPVEGKEPGT VALVSKVGYK LHGRTLRPAL VGVVKEA
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins.
Gene References into Functions
  1. Data suggest that two putative NEF (nucleotide exchange factors) orthologs, GRPEL1 and GRPEL2, modulate function of mitochondrial HSP70 (mtHSP70); both GRPEL1 and GRPEL2 associate with mtHSP70 as hetero-oligomeric subcomplex and regulate mtHSP70 transport. (GRPEL = mitochondrial GrpE-like protein; HSP70 = heat-shock protein 70) PMID: 28848044
  2. The low resolution structure of human GrpE presented suggests that GrpE is a symmetric dimer when not bound to DnaK PMID: 12840016
Subcellular Location
Mitochondrion matrix.
Protein Families
GrpE family
Database Links

HGNC: 19696

OMIM: 606173

KEGG: hsa:80273

STRING: 9606.ENSP00000264954

UniGene: Hs.443723

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